Label-free biosensing with single-molecule force spectroscopy

2013 ◽  
Vol 49 (31) ◽  
pp. 3239 ◽  
Author(s):  
Gang Wei ◽  
Sascha Steckbeck ◽  
Susan Köppen ◽  
Lucio Colombi Ciacchi
Keyword(s):  
Single Molecule ◽  
Force Spectroscopy ◽  
Label Free ◽  
Single Molecule Force Spectroscopy

scholarly journals Molecular Recognition by Silicon Nanowire Field-Effect Transistor and Single-Molecule Force Spectroscopy

Micromachines ◽  
2022 ◽  
Vol 13 (1) ◽  
pp. 97
Author(s):  
Francisco M. Espinosa ◽  
Manuel R. Uhlig ◽  
Ricardo Garcia
Keyword(s):  
Molecular Recognition ◽  
Single Molecule ◽  
Field Effect ◽  
Field Effect Transistors ◽  
Silicon Nanowire ◽  
Force Spectroscopy ◽  
Label Free ◽  
Electrical Measurements ◽  
Single Molecule Force Spectroscopy ◽  
Adsorption Sites

Silicon nanowire (SiNW) field-effect transistors (FETs) have been developed as very sensitive and label-free biomolecular sensors. The detection principle operating in a SiNW biosensor is indirect. The biomolecules are detected by measuring the changes in the current through the transistor. Those changes are produced by the electrical field created by the biomolecule. Here, we have combined nanolithography, chemical functionalization, electrical measurements and molecular recognition methods to correlate the current measured by the SiNW transistor with the presence of specific molecular recognition events on the surface of the SiNW. Oxidation scanning probe lithography (o-SPL) was applied to fabricate sub-12 nm SiNW field-effect transistors. The devices were applied to detect very small concentrations of proteins (500 pM). Atomic force microscopy (AFM) single-molecule force spectroscopy (SMFS) experiments allowed the identification of the protein adsorption sites on the surface of the nanowire. We detected specific interactions between the biotin-functionalized AFM tip and individual avidin molecules adsorbed to the SiNW. The measurements confirmed that electrical current changes measured by the device were associated with the deposition of avidin molecules.

Force spectroscopic detection of peptide cleavage by thrombin exploiting biotin–streptavidin interactions in a bio-sensing context

2019 ◽  
Vol 11 (8) ◽  
pp. 1102-1110 ◽  
Author(s):  
Jingfeng Li ◽  
Qing Li ◽  
Sebastian Potthoff ◽  
Gang Wei ◽  
Colombi Ciacchi Lucio
Keyword(s):  
Atomic Force Microscopy ◽  
Single Molecule ◽  
Force Spectroscopy ◽  
Label Free ◽  
Single Molecule Force Spectroscopy ◽  
Peptide Cleavage ◽  
Force Microscopy ◽  
Atomic Force

Atomic-force-microscopy-based single-molecule force spectroscopy (AFM-SMFS) has become an important technique as the basis of novel, label-free biosensing strategies.

scholarly journals Single Molecule Force Spectroscopy of Siloxanes

2021 ◽  
Vol 714 (3) ◽  
pp. 032023
Author(s):  
Ling Chen ◽  
Liya Yang ◽  
Chunxia Wang ◽  
Ting Zhu
Keyword(s):  
Single Molecule ◽  
Force Spectroscopy ◽  
Single Molecule Force Spectroscopy

Pulling Genetic RNA out of Tobacco Mosaic Virus Using Single-Molecule Force Spectroscopy

2010 ◽  
Vol 132 (32) ◽  
pp. 11036-11038 ◽  
Author(s):  
Ningning Liu ◽  
Bo Peng ◽  
Yuan Lin ◽  
Zhaohui Su ◽  
Zhongwei Niu ◽  
...  
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Single Molecule ◽  
Force Spectroscopy ◽  
Single Molecule Force Spectroscopy

The Nature of the Force-Induced Conformation Transition of dsDNA Studied by Using Single Molecule Force Spectroscopy

Langmuir ◽  
2010 ◽  
Vol 26 (12) ◽  
pp. 9491-9496 ◽  
Author(s):  
Ningning Liu ◽  
Tianjia Bu ◽  
Yu Song ◽  
Wei Zhang ◽  
Jinjing Li ◽  
...  
Keyword(s):  
Single Molecule ◽  
Force Spectroscopy ◽  
Single Molecule Force Spectroscopy ◽  
Conformation Transition

Peptide Desorption Kinetics from Single Molecule Force Spectroscopy Studies

2014 ◽  
Vol 136 (2) ◽  
pp. 688-697 ◽  
Author(s):  
Stefanie Krysiak ◽  
Susanne Liese ◽  
Roland R. Netz ◽  
Thorsten Hugel
Keyword(s):  
Single Molecule ◽  
Force Spectroscopy ◽  
Desorption Kinetics ◽  
Single Molecule Force Spectroscopy ◽  
Spectroscopy Studies
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