Mussel-inspired bioactive ceramics with improved bioactivity, cell proliferation, differentiation and bone-related gene expression of MC3T3 cells

2013 ◽  
Vol 1 (9) ◽  
pp. 933 ◽  
Author(s):  
Mengchi Xu ◽  
Yufeng Zhang ◽  
Dong Zhai ◽  
Jiang Chang ◽  
Chengtie Wu
Keyword(s):  
Gene Expression ◽  
Cell Proliferation ◽  
Related Gene ◽  
Bioactive Ceramics

scholarly journals Effect of Retinoic Acid on Cell Proliferation-Related Gene Expression in Raji Cells Induced by 12-O-tetra-Decanoylphorbol-13-Acetate.

2001 ◽  
Vol 30 ◽  
pp. 21-31
Author(s):  
Eiichiro ICHIISHI ◽  
Toshikazu YOSHIKAWA ◽  
Takako TAKAI ◽  
Harukuni TOKUDA ◽  
Yasuhiko YOSHIDA ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Proliferation ◽  
Retinoic Acid ◽  
Related Gene ◽  
Raji Cells

152 PROLIFERATION- AND SENESCENCE-RELATED GENE EXPRESSION IN CANINE FIBROBLASTS DERIVED FROM DOGS OF DIFFERENT AGE

2016 ◽  
Vol 28 (2) ◽  
pp. 206
Author(s):  
Y. B. Choi ◽  
G. A. Kim ◽  
H. J. Oh ◽  
M. J. Kim ◽  
Y. K. Jo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Proliferation ◽  
Somatic Cell ◽  
Cell Size ◽  
Growth Pattern ◽  
Doubling Time ◽  
Related Gene ◽  
Cell Donor ◽  
Significant Difference ◽  
Old Donor

In pig, cattle and mouse cloning, the age of the cell donor can influence cloning efficiency. However, little is known regarding the effects of the cell donor’s age related to cell proliferation and senescence and its importance for canine cloning. Therefore, the aim of this study was to investigate the effect of cell donor age on growth pattern and doubling time of cell populations, cell size, viability, and senescence-related gene expression (p53, p16, p21, Rb1, and E2F1) in canine fibroblasts. Cells were derived from 1-year-old and 7-year-old dogs. To obtain the fibroblasts, primary culture was performed using ear biopsies from three 1-year-old dogs (1yr) and three 7-year-old dogs (7yr), and cells at passage 2 to 6 were used for somatic cell cloning. Cells were plated at 1 × 105 cells well–1 and harvested every 24 h for 6 days. Cell number was determined for measurement of growth pattern and doubling time. The harvested cells were stained with trypan blue and their size and viability were analysed using a Countess automated cell counter (ThermoFisher Scientific, Waltham, MA, USA). Gene expression was measured by RT-qPCR. Cell proliferation and gene expression experiments were technically replicated 3 and 4 times, respectively. Statistical analysis was performed using GraphPad Prism (GraphPad Software, San Diego, CA, USA) and t-test (P < 0.05) was used. Growth curves in both groups showed typical S-shape and at Day 6, 1yr samples had significantly higher cell numbers compared with 7yr samples. For doubling times, passages 2 to 5 of 1yr (29.8 ± 4.4 h, 41.0 ± 1.6 h, 49.5 ± 4.8 h, and 71.3 ± 8.4 h) were significantly shorter than those of 7yr (47.0 ± 4.5 h, 57.1 ± 2.3 h, 65.8 ± 1.8 h, and 126.4 ± 12.4 h). Cells from passage 6 of 7yr and passage 7 of 1yr did not reach confluence at Day 6. Cell size of 1yr (14.1 ± 0.2 µm) was significantly smaller than that of 7yr (15.3 ± 0.2 µm). Additionally, there was no significant difference in cell viability between 1yr (90.7 ± 0.8%) and 7yr (88.7 ± 0.7%). However, mRNA expression of p53, p21, and Rb1 was significantly lower in 1yr cells compared with 7yr cells. The expression level of E2F1 was significantly higher in 1yr cells. There was no significant difference between 1yr and 7yr cells for p16 expression. In conclusion, proliferation analysis indicated that somatic cells derived from old donor dogs reach a state of senescence more rapidly compared with those of young donor dogs. In addition, analysis of gene expression demonstrated that cells from old donor dogs have significantly increased p53, p21, and Rb1 expression levels, which indicate senescence. Further studies are needed to produce dogs using 1yr and 7yr cells to evaluate the effect of donor aging in canine somatic cell nuclear transfer efficiency. This study was supported by RAD (#PJ0109282015), IPET (#311062-04-3-SB010), Nature Cell, Research Institute for Veterinary Science, (Natural Balance Korea or Nestle Purina Korea) and the BK21 plus program. We thank Barry D. Bavister for his valuable editing of the manuscript.

The effect of iloprost on cell proliferation and angiogenesis-related gene expression in human periodontal ligament cells

Odontology ◽  
2017 ◽  
Vol 106 (1) ◽  
pp. 11-18 ◽  
Author(s):  
Thanomsuk Jearanaiphaisarn ◽  
Teeranuch Sanharati ◽  
Prasit Pavasant ◽  
Chalida Nakalekha Limjeerajarus
Keyword(s):  
Gene Expression ◽  
Cell Proliferation ◽  
Periodontal Ligament ◽  
Periodontal Ligament Cells ◽  
Related Gene ◽  
Human Periodontal Ligament Cells ◽  
Angiogenesis Related Gene

scholarly journals Age-related islet inflammation marks the proliferative decline of pancreatic beta-cells in zebrafish

eLife ◽  
2018 ◽  
Vol 7 ◽  
Author(s):  
Sharan Janjuha ◽  
Sumeet Pal Singh ◽  
Anastasia Tsakmaki ◽  
S Neda Mousavy Gharavy ◽  
Priyanka Murawala ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Proliferation ◽  
Beta Cell ◽  
Beta Cells ◽  
Pancreatic Beta Cells ◽  
Beta Cell Proliferation ◽  
Related Gene ◽  
Age Related ◽  
Islet Inflammation ◽  
Low Activity

The pancreatic islet, a cellular community harboring the insulin-producing beta-cells, is known to undergo age-related alterations. However, only a handful of signals associated with aging have been identified. By comparing beta-cells from younger and older zebrafish, here we show that the aging islets exhibit signs of chronic inflammation. These include recruitment of tnfα-expressing macrophages and the activation of NF-kB signaling in beta-cells. Using a transgenic reporter, we show that NF-kB activity is undetectable in juvenile beta-cells, whereas cells from older fish exhibit heterogeneous NF-kB activity. We link this heterogeneity to differences in gene expression and proliferation. Beta-cells with high NF-kB signaling proliferate significantly less compared to their neighbors with low activity. The NF-kB signalinghi cells also exhibit premature upregulation of socs2, an age-related gene that inhibits beta-cell proliferation. Together, our results show that NF-kB activity marks the asynchronous decline in beta-cell proliferation with advancing age.

ACE INHIBITION ATTENUATES RENAL PDGF GENE EXPRESSION AND CELL PROLIFERATION IN SUBTOTAL NEPHRECTOMY

Nephrology ◽  
2000 ◽  
Vol 5 (3) ◽  
pp. A104-A104
Author(s):  
Jandeleit‐Dahm K ◽  
Wu Ll ◽  
Johnson Rj ◽  
Cox Aj ◽  
Kelly Dj ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Proliferation ◽  
Ace Inhibition ◽  
Subtotal Nephrectomy
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