Analysis of dialkyl urine metabolites of organophosphate pesticides by a liquid chromatography mass spectrometry technique

2014 ◽  
Vol 6 (6) ◽  
pp. 1825-1834 ◽  
Author(s):  
Sukesh Narayan Sinha ◽  
B. Venkat Reddy ◽  
Kasturi Vasudev ◽  
M. Vishnu Vardhana Rao ◽  
M. Noor Ahmed ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Organophosphate Pesticides ◽  
Liquid Chromatography Mass Spectrometry ◽  
Urine Metabolites ◽  
Chromatography Mass Spectrometry ◽  
Mass Spectrometry Technique ◽  
Spectrometry Technique

LC-MS chromatogram of metabolites and their respective structures.

scholarly journals Correction: Analysis of dialkyl urine metabolites of organophosphate pesticides by a liquid chromatography mass spectrometry technique

2018 ◽  
Vol 10 (36) ◽  
pp. 4471-4471
Author(s):  
Sukesh Narayan Sinha ◽  
B. Venkat Reddy ◽  
Kasturi Vasudev ◽  
M. Vishnu Vardhana Rao ◽  
M. Noor Ahmed ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Organophosphate Pesticides ◽  
Liquid Chromatography Mass Spectrometry ◽  
Urine Metabolites ◽  
Chromatography Mass Spectrometry ◽  
Mass Spectrometry Technique ◽  
Spectrometry Technique

Correction for ‘Analysis of dialkyl urine metabolites of organophosphate pesticides by a liquid chromatography mass spectrometry technique’ by Sukesh Narayan Sinha et al., Anal. Methods, 2014, 6, 1825–1834.

scholarly journals Compensate for or Minimize Matrix Effects? Strategies for Overcoming Matrix Effects in Liquid Chromatography-Mass Spectrometry Technique: A Tutorial Review

Molecules ◽  
2020 ◽  
Vol 25 (13) ◽  
pp. 3047 ◽  
Author(s):  
Manuela Cortese ◽  
Maria Rosa Gigliobianco ◽  
Federico Magnoni ◽  
Roberta Censi ◽  
Piera Di Martino
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
High Performance ◽  
Matrix Effects ◽  
Food Science ◽  
Liquid Chromatography Mass Spectrometry ◽  
Internal Standards ◽  
Mass Spectrometry Technique ◽  
The Matrix ◽  
Spectrometry Technique

In recent decades, mass spectrometry techniques, particularly when combined with separation methods such as high-performance liquid chromatography, have become increasingly important in pharmaceutical, bio-analytical, environmental, and food science applications because they afford high selectivity and sensitivity. However, mass spectrometry has limitations due to the matrix effects (ME), which can be particularly marked in complex mixes, when the analyte co-elutes together with other molecules, altering analysis results quantitatively. This may be detrimental during method validation, negatively affecting reproducibility, linearity, selectivity, accuracy, and sensitivity. Starting from literature and own experience, this review intends to provide a simple guideline for selecting the best operative conditions to overcome matrix effects in LC-MS techniques, to obtain the best result in the shortest time. The proposed methodology can be of benefit in different sectors, such as pharmaceutical, bio-analytical, environmental, and food sciences. Depending on the required sensitivity, analysts may minimize or compensate for ME. When sensitivity is crucial, analysis must try to minimize ME by adjusting MS parameters, chromatographic conditions, or optimizing clean-up. On the contrary, to compensate for ME analysts should have recourse to calibration approaches depending on the availability of blank matrix. When blank matrices are available, calibration can occur through isotope labeled internal standards and matrix matched calibration standards; conversely, when blank matrices are not available, calibration can be performed through isotope labeled internal standards, background subtraction, or surrogate matrices. In any case, an adjusting of MS parameters, chromatographic conditions, or a clean-up are necessary.

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