Simple and highly sensitive molecular beacon probe based on target-induced structure-switching DNA for mercury(ii) detection

2013 ◽  
Vol 5 (23) ◽  
pp. 6762 ◽  
Author(s):  
Mei Li ◽  
Yong Huang ◽  
Shulin Zhao
Keyword(s):  
Molecular Beacon ◽  
Highly Sensitive ◽  
Structure Switching ◽  
Induced Structure

Target-induced structure switching of DNA for label-free and ultrasensitive electrochemiluminescent detection of proteins

2014 ◽  
Vol 50 (24) ◽  
pp. 3211-3213 ◽  
Author(s):  
Mengli Yang ◽  
Ying Chen ◽  
Yun Xiang ◽  
Ruo Yuan ◽  
Yaqin Chai
Keyword(s):  
Dna Structure ◽  
Label Free ◽  
Switching Strategy ◽  
Highly Sensitive ◽  
Label Free Detection ◽  
Exo Iii ◽  
Recycling Amplification ◽  
Structure Switching ◽  
Induced Structure

Highly sensitive and label-free detection of thrombin is achieved via a target-induced DNA structure switching strategy and Exo III-assisted recycling amplification.

Target-induced structure switching of aptamers facilitates strand displacement for DNAzyme recycling amplification detection of thrombin in human serum

The Analyst ◽  
2019 ◽  
Vol 144 (7) ◽  
pp. 2430-2435 ◽  
Author(s):  
Jin Li ◽  
Sujing Wang ◽  
Bingying Jiang ◽  
Yun Xiang ◽  
Ruo Yuan
Keyword(s):  
Human Serum ◽  
Strand Displacement ◽  
Recycling Amplification ◽  
Structure Switching ◽  
Induced Structure

Target-induced sequence proximity leads to strand displacement formation of DNAzymes for amplified detection of thrombin in human serum.

Molecular Beacon Enables Combination of Highly Processive and Highly Sensitive Rolling Circle Amplification Readouts for Detection of DNA-Modifying Enzymes

Nano LIFE ◽  
2015 ◽  
Vol 05 (02) ◽  
pp. 1541002 ◽  
Author(s):  
Emil L. Kristoffersen ◽  
Maria Gonzalez ◽  
Magnus Stougaard ◽  
Cinzia Tesauro
Keyword(s):  
Real Time ◽  
Dna Cleavage ◽  
Topoisomerase I ◽  
Drug Response ◽  
Molecular Beacon ◽  
Rolling Circle Amplification ◽  
Dna Topoisomerase ◽  
Rolling Circle ◽  
Topoisomerase Ib ◽  
Highly Sensitive

Here we present an optimized readout format for detection of the circularized products from a DNA-based sensor for measurement of DNA-modifying enzymes including DNA Topoisomerase I. The basic design of the DNA-sensor relies on the use of a substrate that can be circularized by the activity of DNA-modifying enzymes like type IB Topoisomerases and subsequently amplified by a rolling circle amplification (RCA) mechanism. The RCA process can be followed in real-time by the addition of a molecular beacon with a fluorophore/quencher pair. Upon hybridization to the amplified product, the fluorophore/quencher pair is separated, giving rise to a fluorescent signal, measurable in pseudo real-time using a qPCR machine or in a fluorimeter. The RCA products in complex with the molecular beacon can subsequently be moved to microscopic slides and analyzed in a fluorescence microscope. We describe the proof of the principle of this molecular beacon-based method combining the qPCR readout format with the standard Rolling circle Enhanced Enzymatic Assay previously reported. Although the qPCR setup is less sensitive, it allows easy, fast, and high-throughput measurement of enzyme activities. Human Topoisomerase IB (TopIB) is a well-known target for the clinically used anticancer drugs of the camptothecin family. The cytotoxic effect of camptothecins correlates directly with the intracellular TopIB activity affecting reversibly the Topoisomerase/DNA cleavage complexes. Therefore, we envisioned that the presented method may find use for the prediction of cellular drug response and for drug screening to discover novel molecules that specifically inhibit TopIB or other DNA-modifying enzymes.

A novel label-free fluorescent molecular beacon for the detection of 3′–5′ exonuclease enzymatic activity using DNA-templated copper nanoclusters

2017 ◽  
Vol 41 (18) ◽  
pp. 9718-9723 ◽  
Author(s):  
Jia Ge ◽  
Zhen-Zhen Dong ◽  
Dong-Mei Bai ◽  
Lin Zhang ◽  
Ya-Lei Hu ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Molecular Beacon ◽  
Label Free ◽  
Copper Nanoclusters ◽  
Selective Determination ◽  
Highly Sensitive ◽  
Exo Iii

A label-free biosensor was developed for highly sensitive and selective determination of Exo III based on poly(T) molecular beacon-templated CuNPs.

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