Development of supercritical fluid (carbon dioxide) based ultra performance convergence chromatographic stability indicating assay method for the determination of clofarabine in injection

2013 ◽  
Vol 5 (24) ◽  
pp. 7008 ◽  
Author(s):  
Venkata Narasimha Rao Ganipisetty ◽  
Jalandhar D ◽  
Gnana dev G ◽  
Rajendar Bandari ◽  
Manoj P ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Supercritical Fluid ◽  
Assay Method ◽  
Stability Indicating ◽  
Supercritical Fluid Carbon Dioxide

Supercritical fluid (carbon dioxide) based ultra performance convergence chromatography for the separation and determination of fulvestrant diastereomers

2013 ◽  
Vol 5 (18) ◽  
pp. 4832 ◽  
Author(s):  
Ganipisetty Venkata Narasimha Rao ◽  
G. Gnanadev ◽  
Bellam Ravi ◽  
D. Dhananjaya ◽  
P. Manoj ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Supercritical Fluid ◽  
Supercritical Fluid Carbon Dioxide

Development of Validated Stability Indicating HPTLC Method for Assay of Ozagrel and its Pharmaceutical Formulations

2018 ◽  
pp. 36-48 ◽  
Author(s):  
Vishal N Kushare ◽  
Sachin S Kushare
Keyword(s):  
High Performance ◽  
Linear Regression Analysis ◽  
Pharmaceutical Formulations ◽  
Base Hydrolysis ◽  
Assay Method ◽  
Related Substance ◽  
Stability Indicating ◽  
Regular Production ◽  
Hptlc Method

The present paper describes stability indicating high-performance thin-layer chromatography (HPTLC) assay method for Ozagrel in bulk drugs. The method employed TLC aluminium plates precoated with silica gel 60F-254 as the stationary phase. The solvent system consisted of toluene: methanol: triethylamine (6.5: 4.0: 0.1 v/v/v). The system was found to give compact spot for Ozagrel (Rf value of 0.40 ± 0.010). Densitometric analysis of Ozagrel was carried out in the absorbance mode at 280 nm. The linear regression analysis data for the calibration plots showed good linear relationship with r2 = 0.999 with respect to peak area in the concentration range 30 - 120 ng/spot. The developed HPTLC method was validated with respect to accuracy, precision, recovery and robustness. Also to determine related substance and assay determination of Ozagrel that can be used to evaluate the quality of regular production samples. The developed method can also be conveniently used for the assay determination of Ozagrel in pharmaceutical formulations. The limits of detection and quantitation were 4.069 and 12.332 ng/spot, respectively by height. Ozagrel was subjected to acid and alkali hydrolysis, oxidation, photochemical and thermal degradation. The drug undergoes degradation under acidic, basic, oxidation and heat conditions. This indicates that the drug is susceptible to acid, base hydrolysis, oxidation and heat. Statistical analysis proves that the method is repeatable, selective and accurate for the estimation of said drug. The proposed developed HPTLC method can be applied for identification and quantitative determination of Ozagrel in bulk drug and tablet formulation.

Stability Indicating Liquid Chromatographic Method for the Determination of Fenofibrate and its Application to Kinetic Studies

2013 ◽  
Vol 5 (4) ◽  
pp. 1866-1874
Author(s):  
K. Srinivasa Rao ◽  
Keshar N K ◽  
N Jena ◽  
M.E.B Rao ◽  
A K Patnaik
Keyword(s):  
Degradation Products ◽  
Kinetic Studies ◽  
Pharmaceutical Formulations ◽  
Assay Method ◽  
Pharmaceutical Dosage Form ◽  
Stability Indicating ◽  
Zero Order Kinetics ◽  
Relative Standard ◽  
Liquid Chromatographic

A stability-indicating LC assay method was developed for the quantitative determination of fenofibrate (FFB) in pharmaceutical dosage form in the presence of its degradation products and kinetic determinations were evaluated in acidic, alkaline and peroxide degradation conditions. Chromatographic separation was achieved by use of Zorbax C18 column (250 × 4.0 mm, 5 μm). The mobile phase was established by mixing phosphate buffer (pH adjusted 3 with phosphoric acid) and acetonitrile (30:70 v/v). FFB degraded in acidic, alkaline and hydrogen peroxide conditions, while it was more stable in thermal and photolytic conditions. The described method was linear over a range of 1.0-500 μg/ml for determination of FFB (r= 0.9999). The precision was demonstrated by relative standard deviation (RSD) of intra-day (RSD= 0.56– 0.91) and inter-day studies (RSD= 1.47). The mean recovery was found to be 100.01%. The acid and alkaline degradations of FFB in 1M HCl and 1M NaOH solutions showed an apparent zero-order kinetics with rate constants 0.0736 and 0.0698  min−1 respectively and the peroxide degradation with 5% H2O2 demonstrated an apparent first-order kinetics with rate constant k = 0.0202 per min. The t1/2, t90   values are also determined for all the kinetic studies. The developed method was found to be simple, specific, robust, linear, precise, and accurate for the determination of FFB in pharmaceutical formulations.  

Cu(ii) extraction by supercritical fluid carbon dioxide from a room temperature ionic liquid using fluorinated β-diketones

2005 ◽  
Vol 7 (6) ◽  
pp. 421 ◽  
Author(s):  
Soufiane Mekki ◽  
Chien M. Wai ◽  
Isabelle Billard ◽  
Gilles Moutiers ◽  
Clive H. Yen ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Ionic Liquid ◽  
Supercritical Fluid ◽  
Room Temperature ◽  
Room Temperature Ionic Liquid ◽  
Supercritical Fluid Carbon Dioxide

Transesterification of palm pressed-fibers (Elaeis guineensis Jacq.) oil by supercritical fluid carbon dioxide with entrainer ethanol

2018 ◽  
Vol 136 ◽  
pp. 136-143 ◽  
Author(s):  
Fernanda Wariss Figueiredo Bezerra ◽  
Wanessa Almeida da Costa ◽  
Mozaniel Santana de Oliveira ◽  
Eloisa Helena de Aguiar Andrade ◽  
Raul Nunes de Carvalho
Keyword(s):  
Carbon Dioxide ◽  
Supercritical Fluid ◽  
Elaeis Guineensis ◽  
Elaeis Guineensis Jacq ◽  
Supercritical Fluid Carbon Dioxide
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