Simple density-based particle separation in a microfluidic chip

2014 ◽  
Vol 6 (1) ◽  
pp. 308-311 ◽  
Author(s):  
Daisuke Sugiyama ◽  
Yuki Teshima ◽  
Kenichi Yamanaka ◽  
Maria Portia Briones-Nagata ◽  
Masatoshi Maeki ◽  
...  
Keyword(s):  
Microfluidic Chip ◽  
Particle Separation

T1501-2-4 Continuous Particle Separation Using Magnetically Driven Microtool in Microfluidic Chip

2009 ◽  
Vol 2009.8 (0) ◽  
pp. 227-228
Author(s):  
Hisataka MARUYAMA ◽  
Shinya SAKUMA ◽  
Benoit CHAPURLAT ◽  
Yoko YAMANISHI ◽  
Fumihito ARAI
Keyword(s):  
Microfluidic Chip ◽  
Particle Separation ◽  
Continuous Particle

scholarly journals Simultaneous and continuous particle separation and counting via localized DC-dielectrophoresis in a microfluidic chip

RSC Advances ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 3827-3833
Author(s):  
Yongxin Song ◽  
Xiaoshi Han ◽  
Deyu Li ◽  
Qinxin Liu ◽  
Dongqing Li
Keyword(s):  
Microfluidic Chip ◽  
Particle Separation ◽  
First Report ◽  
Particle Counting ◽  
Continuous Particle ◽  
Dc Dielectrophoresis

The first report that particle counting and separation can be achieved simultaneously. Separation and counting of polystyrene particles of two and three different sizes with 1 μm resolution were demonstrated experimentally.

Modular Microfluidic Filters Based on Transparent Membranes

2016 ◽  
Vol 138 (4) ◽  
Author(s):  
E. Archibong ◽  
H. Tuazon ◽  
H. Wang ◽  
J. Winskas ◽  
A. L. Pyayt
Keyword(s):  
Microfluidic Chip ◽  
Membrane Damage ◽  
Particle Separation ◽  
Live Cells ◽  
New Approach ◽  
Silicon Nitride Membrane ◽  
Particle Imaging ◽  
Silicon Based ◽  
Functional Components ◽  
Low Pressures

We propose a new approach to the modular packaging of microfluidic components, in which different functional components are not only fabricated separately but are also designed to be individually removable for the purposes of replacement or subsequent analysis. In this paper, we demonstrate one such component: a stand-alone microfluidic filter that can be custom-fabricated and then connected, disconnected, and replaced on a microfluidic chip as needed. This filter is also designed such that particles captured on the filter can be further analyzed or processed directly on the filter itself—for example, for microscopic examination or cell culturing. The filter is a thin (1 μm) transparent silicon nitride membrane that can be designed and fabricated according to specifications for different applications. This material is suitable for microscale fabrication; filtration of a variety of solutions, including biological samples; and subsequent particle imaging and processing. The porous nature of the thin filter allows for particle separation under relatively low pressures, thus protecting the particles from rupture or membrane damage. We describe two methods for integrating the filter apparatus onto a microfluidic chip such that it can be inserted, removed, and replaced. To demonstrate the utility of this approach, we fabricated custom-designed silicon-based filters, incorporated them onto microfluidic systems then filtered microparticles and live cells from test solutions, and finally removed the filters to image the microparticles and culture the cells directly on the filter membranes.

Drop driving on digital microfluidic chip

2020 ◽  
Vol 28 (11) ◽  
pp. 2488-2496
Author(s):  
Hong WANG ◽  
◽  
Jie ZHENG ◽  
Yan-peng YAN ◽  
Song WANG ◽  
...  
Keyword(s):  
Microfluidic Chip ◽  
Digital Microfluidic

An Enrichment Method for Bio-samples Based on Circular Injection on a Microfluidic Chip

2013 ◽  
Vol 40 (11) ◽  
pp. 1668-1673
Author(s):  
Min DU ◽  
Xiong-Ying YE ◽  
Jin-Yang FENG ◽  
Zeng-Shuai MA ◽  
Zhao-Ying ZHOU
Keyword(s):  
Microfluidic Chip ◽  
Enrichment Method

An enzyme reactor based on aptamer modified microfluidic chip for protein analysis

2013 ◽  
Vol 30 (11) ◽  
pp. 1127-1132 ◽  
Author(s):  
Peng XIAO ◽  
Dalei LI ◽  
Yan MAN ◽  
Lina GENG ◽  
Xuefei LU ◽  
...  
Keyword(s):  
Microfluidic Chip ◽  
Protein Analysis ◽  
Enzyme Reactor

Prefiltration/Clarification via Dynamic Particle Separation

2015 ◽  
Vol 2 (0) ◽  
pp. 9781843396703-9781843396703
Author(s):  
S. R. Wright ◽  
S. Crouch ◽  
D. Wesson ◽  
S. Grady
Keyword(s):  
Particle Separation

scholarly journals Microfluidic Chip for Detection of Fungal Infections

ACS Omega ◽  
2019 ◽  
Vol 4 (4) ◽  
pp. 7474-7481 ◽  
Author(s):  
Waseem Asghar ◽  
Mazhar Sher ◽  
Nida S. Khan ◽  
Jatin M. Vyas ◽  
Utkan Demirci
Keyword(s):  
Microfluidic Chip ◽  
Fungal Infections

scholarly journals An LED-Driven AuNPs-PDMS Microfluidic Chip and Integrated Device for the Detection of Digital Loop-Mediated Isothermal DNA Amplification

Micromachines ◽  
2020 ◽  
Vol 11 (2) ◽  
pp. 177 ◽  
Author(s):  
Zengming Zhang ◽  
Shuhao Zhao ◽  
Fei Hu ◽  
Guangpu Yang ◽  
Juan Li ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Microfluidic Chip ◽  
Cross Flow ◽  
Flow Channel ◽  
Isothermal Amplification ◽  
Fluorescent Detection ◽  
Lamp Method ◽  
Biological Studies ◽  
Micro Cavity ◽  
Integrated Device

The sensitive quantification of low-abundance nucleic acids holds importance for a range of clinical applications and biological studies. In this study, we describe a facile microfluidic chip for absolute DNA quantifications based on the digital loop-mediated isothermal amplification (digital LAMP) method. This microfluidic chip integrates a cross-flow channel for droplet generation with a micro-cavity for droplet tiling. DNA templates in the LAMP reagent were divided into ~20,000 water-in-oil droplets at the cross-flow channel. The droplets were then tiled in the micro-cavity for isothermal amplification and fluorescent detection. Different from the existing polydimethylsiloxane (PDMS) microfluidic chips, this study incorporates gold nanoparticles (AuNPs) into PDMS substrate through silica coating and dodecanol modification. The digital LAMP chip prepared by AuNPs-PDMS combines the benefits of the microstructure manufacturing performance of PDMS with the light-to-heat conversion advantages of AuNPs. Upon illumination with a near infrared (NIR) LED, the droplets were stably and efficiently heated by the AuNPs in PDMS. We further introduce an integrated device with a NIR heating unit and a fluorescent detection unit. The system could detect HBV (hepatitis B virus)-DNA at a concentration of 1 × 101 to 1 × 104 copies/μL. The LED-driven digital LAMP chip and the integrated device; therefore, demonstrate high accuracy and excellent performance for the absolute quantification of low-abundance nucleic acids, showing the advantages of integration, miniaturization, cost, and power consumption.

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