An indirect competitive enzyme-linked immunosorbent assay for bisphenol-A based on the synthesis of a poly-l-lysine–hapten conjugate as a coating antigen

2013 ◽  
Vol 5 (6) ◽  
pp. 1570 ◽  
Author(s):  
Jianqing Zhou ◽  
Suqing Zhao ◽  
Jun Zhang ◽  
Lei Zhang ◽  
Yanfei Cai ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Coating Antigen

Development of a Direct Competitive Enzyme-linked Immunosorbent Assay Using a Sensitive Monoclonal Antibody for Bisphenol A

Hybridoma ◽  
2011 ◽  
Vol 30 (1) ◽  
pp. 95-100 ◽  
Author(s):  
Chunmei Ju ◽  
Youhua Xiong ◽  
Aizhong Gao ◽  
Tangbin Yang ◽  
Lei Wang
Keyword(s):  
Monoclonal Antibody ◽  
Bisphenol A ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay

The Development of a Quantitative ELISA for Aflatoxin B1 in Ground Peanuts Using Antibodies Isolated From the Yolk of a Laying Hen

1994 ◽  
Vol 57 (11) ◽  
pp. 1022-1024 ◽  
Author(s):  
SUZHEN LI ◽  
RONALD R. MARQUARDT ◽  
ANDREW A. FROHLICH ◽  
HAO XIAO ◽  
JAMES R. CLARKE
Keyword(s):  
Immunosorbent Assay ◽  
Aflatoxin B1 ◽  
Laying Hens ◽  
Egg Yolk ◽  
Laying Hen ◽  
Competitive Elisa ◽  
Enzyme Linked Immunosorbent Assay ◽  
Coating Antigen ◽  
Extraction Protocol

Antibodies directed against Aflatoxin B1 (AFB1) were produced in laying hens, isolated from egg yolk and applied in an enzyme-linked immunosorbent assay (ELISA) for AFB1 in ground peanuts. The ELISA sensitivity was improved by reduction of the amount of the coating antigen absorbed onto the microplate well surfaces. The main aflatoxins B1, B2, G1, G2, M1, aflatoxicol, sterigmatocystin were found to cross-react in the competitive ELISA, 100, 25, 23, 4, 0, 0, 0%, respectively. Aflatoxin B1 could be reproducibly detected and quantified in spiked ground peanuts at levels greater than 5 ppb using a hexane and methanol solvent based peanut extraction protocol.

Determination of Bisphenol A by a Gold Nanoflower Enhanced Enzyme-Linked Immunosorbent Assay

2016 ◽  
Vol 49 (10) ◽  
pp. 1492-1501 ◽  
Author(s):  
Chifang Peng ◽  
Na Pan ◽  
Zhengjun Xie ◽  
Liqiang Liu ◽  
Jie Xiang ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay

Development of an Enzyme-Linked Immunosorbent Assay for Bisphenol A Using Chicken Immunoglobulins

2002 ◽  
Vol 50 (19) ◽  
pp. 5273-5282 ◽  
Author(s):  
Bruno De Meulenaer ◽  
Katleen Baert ◽  
Heikki Lanckriet ◽  
Vera Van Hoed ◽  
Andre Huyghebaert
Keyword(s):  
Bisphenol A ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay

scholarly journals Quantitative Determination of Acetamiprid in Pollen Based on a Sensitive Enzyme-Linked Immunosorbent Assay

Molecules ◽  
2019 ◽  
Vol 24 (7) ◽  
pp. 1265 ◽  
Author(s):  
Qingkui Fang ◽  
Quan Zu ◽  
Xiude Hua ◽  
Pei Lv ◽  
Wanwen Lin ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Detection Limit ◽  
Quantitative Determination ◽  
Immunosorbent Assay ◽  
Linear Range ◽  
Enzyme Linked Immunosorbent Assay ◽  
Experimental Conditions ◽  
Coating Antigen ◽  
Relative Standard

A sensitive biotinylated indirect competitive enzyme-linked immunosorbent assay (Bic-ELISA) was developed to detect acetamiprid pesticides in pollen, based on the heterogeneous coating antigen and biotinylated anti-acetamiprid monoclonal antibody. Under optimized experimental conditions, the detection limit for the Bic-ELISA was 0.17 ng/mL and the linear range was 0.25–25 ng/mL. The cross-reactivities could be regarded as negligible for the biotinylated antibodies with their analogues except for thiacloprid (1.66%). Analyte recoveries for extracts of spiked pollen (camellia pollen, lotus pollen, rape pollen) ranged from 81.1% to 108.0%, with intra-day relative standard deviations (RSDs) of 4.8% to 10.9%, and the average reproducibility was 85.4% to 110.9% with inter-assay and inter-assay RSDs of 6.1% to 11.7%. The results of Bic-ELISA methods for the Taobao’s website samples were largely consistent with HPLC-MS/MS. Therefore, the established Bic-ELISA methods would be conducive to the monitoring of acetamiprid in pollen.

Enzyme-linked immunosorbent assay for bisphenol A: Assay optimization and its application for surface water analysis

2007 ◽  
Vol 89 (1) ◽  
pp. 161-172 ◽  
Author(s):  
Alexander S. Krapivin ◽  
Jeanne V. Samsonova ◽  
Nataliya A. Uskova ◽  
Nina L. Ivanova ◽  
Alexey M. Egorov
Keyword(s):  
Surface Water ◽  
Bisphenol A ◽  
Immunosorbent Assay ◽  
Water Analysis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Assay Optimization ◽  
Surface Water Analysis

Development of a highly sensitive enzyme-linked immunosorbent assay for bisphenol A in serum

The Analyst ◽  
2001 ◽  
Vol 127 (1) ◽  
pp. 93-97 ◽  
Author(s):  
H. Ohkuma ◽  
K. Abe ◽  
M. Ito ◽  
A. Kokado ◽  
A. Kambegawa ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Highly Sensitive
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