Preparation and electrochromatographic characterization of methacrylate-based weak cation exchange columns for capillary electrochromatography

The Analyst ◽  
2013 ◽  
Vol 138 (7) ◽  
pp. 2118 ◽  
Author(s):  
Cemil Aydoğan ◽  
Adil Denizli
Keyword(s):  
Cation Exchange ◽  
Capillary Electrochromatography ◽  
Weak Cation Exchange

Characterization of the Acidic Species of a Monoclonal Antibody Using Weak Cation Exchange Chromatography and LC-MS

2015 ◽  
Vol 87 (17) ◽  
pp. 9084-9092 ◽  
Author(s):  
Gomathinayagam Ponniah ◽  
Adriana Kita ◽  
Christine Nowak ◽  
Alyssa Neill ◽  
Yekaterina Kori ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Cation Exchange ◽  
Exchange Chromatography ◽  
Cation Exchange Chromatography ◽  
Weak Cation Exchange

scholarly journals Diagnostic model of saliva peptide finger print analysis of oral squamous cell carcinoma patients using weak cation exchange magnetic beads

2015 ◽  
Vol 35 (3) ◽  
Author(s):  
Wei-Peng Jiang ◽  
Zhen Wang ◽  
Li-Xin Xu ◽  
Xin Peng ◽  
Feng Chen
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cation Exchange ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Magnetic Beads ◽  
Diagnostic Model ◽  
Finger Print ◽  
Whole Saliva ◽  
Weak Cation Exchange

Whole saliva (WS) was used for diagnosis of oral squamous cell carcinoma (OSCC); two polypeptides may be used for OSCC diagnosis.

Capillary electrochromatography/nanoelectrospray mass spectrometry for attomole characterization of peptides

2000 ◽  
Vol 14 (16) ◽  
pp. 1448-1454 ◽  
Author(s):  
Marjan Gu??ek ◽  
Marco Gaspari ◽  
Karin Walhagen ◽  
Rob J. Vreeken ◽  
Elwin R. Verheij ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Capillary Electrochromatography

Weak cation-exchange restricted-access material for on-line purification of basic drugs in plasma

2008 ◽  
Vol 1190 (1-2) ◽  
pp. 8-13 ◽  
Author(s):  
Yoshiaki Sato ◽  
Eiichi Yamamoto ◽  
Susumu Takakuwa ◽  
Takashi Kato ◽  
Naoki Asakawa
Keyword(s):  
Cation Exchange ◽  
Basic Drugs ◽  
Restricted Access ◽  
Restricted Access Material ◽  
Weak Cation Exchange ◽  
On Line

scholarly journals Purification and characterization of prolixin S (nitrophorin 2), the salivary anticoagulant of the blood-sucking bug Rhodnius prolixus

1995 ◽  
Vol 308 (1) ◽  
pp. 243-249 ◽  
Author(s):  
J M C Ribeiro ◽  
M Schneider ◽  
J A Guimarães
Keyword(s):  
Cation Exchange ◽  
Salivary Glands ◽  
Coagulation Factor ◽  
Rhodnius Prolixus ◽  
Exchange Chromatography ◽  
Factor X ◽  
Blood Sucking ◽  
Weak Cation Exchange ◽  
Haem Protein ◽  
Strong Cation Exchange Chromatography

The salivary anticoagulant of the blood-sucking bug Rhodnius prolixus was purified to homogeneity using a protocol consisting of weak cation-exchange, DEAE, hydrophobic-interaction and octadecyl reverse-phase chromatography, yielding a protein with the same N-terminal sequence as nitrophorin 2, one of the four NO haem protein carriers present in the salivary glands of Rhodnius with a molecular mass of 19689 Da [D. Champagne, R.H. Nussenzveig and J.M.C. Ribeiro, (1995) J. Biol. Chem. 270, in the press]. To exclude the possibility of the nitrophorin being a contaminant, another chromatographic protocol was performed, consisting of chromatofocusing followed by strong-cation-exchange chromatography. Again the anticoagulant was eluted with nitrophorin 2. Nitrophorin 2 inhibits coagulation Factor VIII-mediated activation of Factor X and accounts for all the anti-clotting activity observed in Rhodnius salivary glands.

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