Single nanoparticle detection using photonic crystal enhanced microscopy

The Analyst ◽  
2014 ◽  
Vol 139 (5) ◽  
pp. 1007-1015 ◽  
Author(s):  
Yue Zhuo ◽  
Huan Hu ◽  
Weili Chen ◽  
Meng Lu ◽  
Limei Tian ◽  
...  
Keyword(s):  
Photonic Crystal ◽  
Metal Nanoparticles ◽  
Crystal Surface ◽  
Label Free ◽  
Surface Attachment ◽  
Nanoparticle Detection ◽  
Single Nanoparticle ◽  
Imaging Approach

We demonstrate a label-free biosensor imaging approach that utilizes a photonic crystal surface to detect surface attachment of individual dielectric and metal nanoparticles.

Photonic crystal surface mode imaging for multiplexed and high-throughput label-free biosensing

2020 ◽  
Vol 168 ◽  
pp. 112575 ◽  
Author(s):  
Valery Konopsky ◽  
Tatiana Mitko ◽  
Konstantin Aldarov ◽  
Elena Alieva ◽  
Dmitry Basmanov ◽  
...  
Keyword(s):  
Photonic Crystal ◽  
High Throughput ◽  
Crystal Surface ◽  
Surface Mode ◽  
Label Free

scholarly journals Label-Free Flow Multiplex Biosensing via Photonic Crystal Surface Mode Detection

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Irina Petrova ◽  
Valery Konopsky ◽  
Igor Nabiev ◽  
Alyona Sukhanova
Keyword(s):  
Photonic Crystal ◽  
Crystal Surface ◽  
Free Flow ◽  
Surface Mode ◽  
Label Free ◽  
Mode Detection

Combined enhanced fluorescence and label-free biomolecular detection with a photonic crystal surface

2007 ◽  
Vol 46 (12) ◽  
pp. 2351 ◽  
Author(s):  
Patrick C. Mathias ◽  
Nikhil Ganesh ◽  
Leo L. Chan ◽  
Brian T. Cunningham
Keyword(s):  
Photonic Crystal ◽  
Crystal Surface ◽  
Label Free ◽  
Enhanced Fluorescence ◽  
Biomolecular Detection

Single-nanoparticle detection with slot-mode photonic crystal cavities

2015 ◽  
Vol 106 (26) ◽  
pp. 261105 ◽  
Author(s):  
Cheng Wang ◽  
Qimin Quan ◽  
Shota Kita ◽  
Yihang Li ◽  
Marko Lončar
Keyword(s):  
Photonic Crystal ◽  
Photonic Crystal Cavities ◽  
Nanoparticle Detection ◽  
Single Nanoparticle

scholarly journals Development of a microfluidic biosensor for the diagnostics and typing of Mycobacterium Tuberculosis

2021 ◽  
Vol 12 (2) ◽  
pp. 14-20
Author(s):  
Tatiana V. Mitko ◽  
Ruslan I. Shakurov ◽  
Fedor V. Shirshikov ◽  
Sizova V. Svetlana ◽  
Elena V. Alieva ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Photonic Crystal ◽  
Crystal Surface ◽  
Specific Binding ◽  
Oligonucleotide Probes ◽  
Label Free ◽  
Single Stranded Dna ◽  
Simultaneous Registration ◽  
Microfluidic Biosensor ◽  
Resistance Markers

Background. Despite on the general trend towards decreasing the incidence of newly diagnosed active forms of tuberculosis, the situation with spreading of this disease in Russian Federation remains extremely tense. At the same time, the diagnosis is carried out according to the standard scheme, which takes about a month; another month takes test formulation for drug sensitivity. Thus, the development of new methods for diagnostics and typing of mycobacteria, as well as practice implementation of these developments is an urgent direction. Modern developments in the field of microfluidic technologies open up great opportunities in this direction. Aim. Development of a method for identification and typing of Mycobacterium tuberculosis using a label-free biosensor on surface waves in a one-dimensional photonic crystal (PC SM biosensor). Methods. Oligonucleotide probes were selected and synthesized as DNA targets for M. tuberculosis typing. The photonic crystal surface was modified with aqueous solutions of (3-aminopropyl)triethoxysilane, Leuconostoc mesenteroides dextrans and bovine serum albumin. Experiments were carried out using a PC SM biosensor. Results. Sequences of detecting oligonucleotide probes were selected for spoligotyping of M. tuberculosis on the PC SM biosensor. Modification of their 3'-ends was carried out in order to create extended single-stranded regions that are not subject to the formation of secondary structures and facilitate hybridization with a single-stranded DNA target. Several series of experimental modifications of the PC surface were carried out by using L. mesenteroides dextrans with different functional groups (including detection of the modification results real time) with simultaneous registration of the increment layer size and volume refractive index of the mixture, which excludes the use of a reference cell. Other experiments were carried out to detect the specific binding of biotinylated oligonucleotide probes to the modified PC surface. Conclusions. A technique for the design of probes was developed and a model system of oligonucleotides for the detection of single-stranded DNA using a PC biosensor was proposed. The developed technique of modification of the PC surface with dextrans from L. mesenteroides, which allows to increase the sensitivity of detection of oligonucleotides using the PC SM biosensor. This approach will further expand the panel of diagnostic probes, including identification of resistance markers.

scholarly journals Multiparametric detection of bacterial contamination based on the photonic crystal surface mode detection

2018 ◽  
pp. 19-24
Author(s):  
I. O. Petrova ◽  
V. N. Konopsky ◽  
A. V. Sukhanova ◽  
I. R. Nabiev
Keyword(s):  
Photonic Crystal ◽  
Crystal Surface ◽  
Surface Mode ◽  
Label Free ◽  
Water Quality Control ◽  
Analytical Technique ◽  
Mode Detection ◽  
Highly Sensitive ◽  
Cell Affinity ◽  
Detection Of Biomarkers

Conventional techniques for food and water quality control and environmental monitoring in general have a number of drawbacks. Below we propose a label-free highly accurate analytical technique for multiplex detection of biomarkers based on the analysis of propagation of Bloch waves on the surface of a photonic crystal. The technique can be used to measure molecular and cell affinity interactions in real time by recording critical and excitation angles of the surface wave on the surface of a photonic crystal. Based on the analysis of photonic crystal surface modes, we elaborated a protocol for the detection of the exotoxin A of Pseudomonas aeruginosa and the heat-labile toxin LT of Escherichia coli. The protocol exploits detection of affinity interactions between antigens pumped through a microfluidic cell and detector antibodies conjugated to the chemically activated silica chip. The proposed technique is highly sensitive, cheap and less time-consuming in comparison with surface plasmon resonance.

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