A mesofluidic platform integrating on-chip probe ultrasonication for multiple sample pretreatment involving denaturation, reduction, and digestion in protein identification assays by mass spectrometry

The Analyst ◽  
2014 ◽  
Vol 139 (5) ◽  
pp. 992-995 ◽  
Author(s):  
J. D. Nunes-Miranda ◽  
Cristina Núñez ◽  
Hugo M. Santos ◽  
G. Vale ◽  
Miguel Reboiro-Jato ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Throughput ◽  
Protein Identification ◽  
Sample Pretreatment ◽  
Multiple Sample ◽  
On Chip

A novel mesofluidic platform integrating on-chip probe ultrasonication for automated high-throughput shotgun proteomic assays.

scholarly journals Obesity Proteomics: An Update on the Strategies and Tools Employed in the Study of Human Obesity

2018 ◽  
Vol 7 (3) ◽  
pp. 27 ◽  
Author(s):  
Afshan Masood ◽  
Hicham Benabdelkamel ◽  
Assim Alfadda
Keyword(s):  
Mass Spectrometry ◽  
High Throughput ◽  
Large Scale ◽  
Protein Identification ◽  
Biochemical Characterization ◽  
Biological Fluids ◽  
Protein Composition ◽  
Cellular Protein ◽  
Human Obesity ◽  
Two Dimensional Gel Electrophoresis

Proteomics has become one of the most important disciplines for characterizing cellular protein composition, building functional linkages between protein molecules, and providing insight into the mechanisms of biological processes in a high-throughput manner. Mass spectrometry-based proteomic advances have made it possible to study human diseases, including obesity, through the identification and biochemical characterization of alterations in proteins that are associated with it and its comorbidities. A sizeable number of proteomic studies have used the combination of large-scale separation techniques, such as high-resolution two-dimensional gel electrophoresis or liquid chromatography in combination with mass spectrometry, for high-throughput protein identification. These studies have applied proteomics to comprehensive biochemical profiling and comparison studies while using different tissues and biological fluids from patients to demonstrate the physiological or pathological adaptations within their proteomes. Further investigations into these proteome-wide alterations will enable us to not only understand the disease pathophysiology, but also to determine signature proteins that can serve as biomarkers for obesity and related diseases. This review examines the different proteomic techniques used to study human obesity and discusses its successful applications along with its technical limitations.

On-chip integration of droplet microfluidics and nanostructure-initiator mass spectrometry for enzyme screening

Lab on a Chip ◽  
2017 ◽  
Vol 17 (2) ◽  
pp. 323-331 ◽  
Author(s):  
Joshua Heinemann ◽  
Kai Deng ◽  
Steve C. C. Shih ◽  
Jian Gao ◽  
Paul D. Adams ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Throughput ◽  
Droplet Microfluidics ◽  
Enzymatic Assay ◽  
Enzyme Screening ◽  
Highly Sensitive ◽  
On Chip

μNIMS, a highly sensitive and high throughput technique for enzymatic assay that integrates droplet microfluidics with nanostructure-initiator mass spectrometry (NIMS).

154: Integration of TPSA and High-Throughput Mass Spectrometry Data Improves Prostate Cancer Prediction

2007 ◽  
Vol 177 (4S) ◽  
pp. 52-53
Author(s):  
Stefano Ongarello ◽  
Eberhard Steiner ◽  
Regina Achleitner ◽  
Isabel Feuerstein ◽  
Birgit Stenzel ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Mass Spectrometry ◽  
High Throughput ◽  
Mass Spectrometry Data ◽  
Cancer Prediction
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