A lateral flow assay for identification of Escherichia coli by ribosomal RNA hybridisation

The Analyst ◽  
2014 ◽  
Vol 139 (5) ◽  
pp. 1063 ◽  
Author(s):  
Christopher Pöhlmann ◽  
Irina Dieser ◽  
Mathias Sprinzl
Keyword(s):  
Escherichia Coli ◽  
Ribosomal Rna ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Rna Hybridisation

Potential Rapid and Simple Lateral Flow Assay for Escherichia coli O111

2013 ◽  
Vol 76 (5) ◽  
pp. 755-761 ◽  
Author(s):  
YOSHITAKA TERAO ◽  
TARO YONEKITA ◽  
NAOKI MORISHITA ◽  
TATSUYA FUJIMURA ◽  
TAKASHI MATSUMOTO ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Direct Detection ◽  
Culture Method ◽  
Test Strip ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
E Coli ◽  
Negative Results ◽  
Meat Samples ◽  
Positive Results

We developed and evaluated a lateral flow assay (LFA) as a simple and rapid method for direct detection of Escherichia coli O111 in food after enrichment. When cell suspensions of 8 E. coli O111 strains and 77 non–E. coli O111 strains were tested with the LFA, the former all yielded positive results and the latter all yielded negative results. The minimum detection limits for the E. coli O111 strains were 1.8 × 103 to 5.6 × 105 CFU/ml of cell suspension, and the LFA was able to detect live cultures or those killed by autoclaving at nearly the same level of sensitivity. To evaluate the ability of LFA to detect its target in food, enrichment cultures of meat samples inoculated with 10-fold serial dilutions of E. coli O111 were tested with the LFA and PCR. Even when there were very few E. coli O111 cells in the meat samples (1.6 × 100 to 1.6 × 101 CFU/25 g of food), when they were cultured in modified E. coli broth with novobiocin for 22 h at 42°C, the LFA yielded positive results that corresponded to the PCR results. Although the LFA requires further evaluation and field study, these results suggest that this assay has sufficient sensitivity and specificity. This procedure can be completed with a one-step incubation after the test strip has been inserted into the sample after 22 h of culture, whereas the standard culture method requires multiple cultures, skilled personnel, a well-equipped laboratory, and 4 or 5 days. The speed and simplicity of this LFA make it suitable for use as part of routine screening assays in the food industry.

scholarly journals Nanozyme-based lateral flow assay for the sensitive detection of Escherichia coli O157:H7 in milk

2018 ◽  
Vol 101 (7) ◽  
pp. 5770-5779 ◽  
Author(s):  
Jiaojiao Han ◽  
Lei Zhang ◽  
Liming Hu ◽  
Keyu Xing ◽  
Xuefei Lu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Sensitive Detection ◽  
Escherichia Coli O157

Development of a novel multiplex lateral flow assay using an antimicrobial peptide for the detection of Shiga toxin-producing Escherichia coli

2013 ◽  
Vol 93 (3) ◽  
pp. 251-256 ◽  
Author(s):  
Taro Yonekita ◽  
Ryuji Ohtsuki ◽  
Eri Hojo ◽  
Naoki Morishita ◽  
Takashi Matsumoto ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Peptide ◽  
Shiga Toxin ◽  
Lateral Flow ◽  
Lateral Flow Assay

Development of a combined immunochromatographic lateral flow assay for accurate and rapid Escherichia coli O157:H7 detection

2020 ◽  
Vol 71 (3) ◽  
pp. 311-319 ◽  
Author(s):  
Y. Ye ◽  
W. Su ◽  
J. Zhang ◽  
Y. Huang ◽  
W. Chen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Escherichia Coli O157
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