scholarly journals Whole organ cross-section chemical imaging using label-free mega-mosaic FTIR microscopy

The Analyst ◽  
2013 ◽  
Vol 138 (23) ◽  
pp. 7066 ◽  
Author(s):  
Paul Bassan ◽  
Ashwin Sachdeva ◽  
Jonathan H. Shanks ◽  
Mick D. Brown ◽  
Noel W. Clarke ◽  
...  
Keyword(s):  
Cross Section ◽  
Chemical Imaging ◽  
Label Free ◽  
Ftir Microscopy

Erratum to: Chemical Heterogeneity of Deposits Formed in the Flocculant Flow From Crude Oil, According to FTIR Microscopy and Chemical Imaging

2021 ◽  
Vol 61 (11) ◽  
pp. 1327-1327
Author(s):  
A. S. Shalygin ◽  
E. S. Milovanov ◽  
S. S. Yakushkin ◽  
O. N. Martyanov
Keyword(s):  
Crude Oil ◽  
Chemical Imaging ◽  
Chemical Heterogeneity ◽  
Ftir Microscopy

scholarly journals Viewing Cancer in a New Light: Multimodal Label-Free Specific Chemical Imaging to Distinguish Healthy Cell Tissue from Invasive Carcinoma

2017 ◽  
Vol 112 (3) ◽  
pp. 187a
Author(s):  
Bjarne Thorsted ◽  
Stine R. Larsen ◽  
Christian Godballe ◽  
Jonathan R. Brewer
Keyword(s):  
Invasive Carcinoma ◽  
Chemical Imaging ◽  
Label Free ◽  
Specific Chemical ◽  
Healthy Cell ◽  
Cell Tissue

Monitoring the effects of chemical stimuli on live cells with metasurface-enhanced infrared reflection spectroscopy

2021 ◽  
Author(s):  
Steven H. Huang ◽  
Jiaruo Li ◽  
Zhiyuan Fan ◽  
Robert Delgado ◽  
Gennady Shvets
Keyword(s):  
Intracellular Signaling ◽  
Strong Field ◽  
Detection System ◽  
Field Enhancement ◽  
Chemical Imaging ◽  
Live Cells ◽  
Label Free ◽  
Reflection Spectroscopy ◽  
Infrared Reflection ◽  
Infrared Reflection Spectroscopy

Infrared spectroscopy has found wide applications in the analysis of biological materials. A more recent development is the use of engineered nanostructures, or plasmonic metasurfaces, as substrates for metasurface-enhanced infrared reflection spectroscopy (MEIRS). Here, we demonstrate that strong field enhancement from plasmonic metasurfaces enables the use of MEIRS as a highly informative analytic technique for real-time monitoring of cells. By exposing live cells cultured on a plasmonic metasurface to chemical compounds, we show that MEIRS can be used as a label-free phenotypic assay for detecting multiple cellular responses to external stimuli: changes in cell morphology, adhesion, lipid composition of the cellular membrane, as well as intracellular signaling. Using a focal plane array detection system, we show that MEIRS also enables spectro-chemical imaging at the single-cell level. The described metasurface-based all-optical sensor opens the way to a scalable, high-throughput spectroscopic assay for live cells.

Tracking the Formation and Degradation of Fatty-Acid-Accumulated Mitochondria Using Label-Free Chemical Imaging

2020 ◽  
Author(s):  
Chi Zhang ◽  
Stephen Boppart
Keyword(s):  
Fatty Acid ◽  
Structural Features ◽  
Chemical Imaging ◽  
Live Cells ◽  
Label Free ◽  
Dynamic Changes ◽  
Regulatory Processes ◽  
Transport Chain ◽  
Anti Stokes ◽  
First Time

Abstract The mitochondrion is one of the key organelles for maintaining cellular homeostasis. External environmental stimuli and internal regulatory processes alter the metabolism and functions of mitochondria. To understand these activities of mitochondria, it is critical to probe the key metabolic molecules inside these organelles. In this study, we used label-free chemical imaging modalities including coherent anti-Stokes Raman scattering and multiphoton-excited autofluorescence to study the mitochondrial activities in living cancer cells. We found that hypothermia exposure tends to induce fatty-acid (FA) accumulation in some mitochondria of MIAPaCa-2 cells. Autofluorescence images show that the FA-accumulated mitochondria also have abnormal NADH and FAD metabolism, likely induced by the dysfunction of the electron transport chain. We also found that when the cells were re-warmed to physiological temperature after a period of hypothermia, the FA-accumulated mitochondria changed their structural features, likely caused by the mitophagy process. To the best of our knowledge, this is the first time that FA accumulation in mitochondria was observed in live cells. Our research also demonstrates that multimodal label-free chemical imaging is an attractive tool to discover abnormal functions of mitochondria at the single-organelle level and can be used to quantify the dynamic changes of this organelle under perturbative conditions.

scholarly journals Label-free chemical imaging flow cytometry by high-speed multicolor stimulated Raman scattering

2019 ◽  
Vol 116 (32) ◽  
pp. 15842-15848 ◽  
Author(s):  
Yuta Suzuki ◽  
Koya Kobayashi ◽  
Yoshifumi Wakisaka ◽  
Dinghuan Deng ◽  
Shunji Tanaka ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Raman Scattering ◽  
Cancer Biology ◽  
High Speed ◽  
Stimulated Raman Scattering ◽  
Chemical Imaging ◽  
Fluorescent Labeling ◽  
Label Free ◽  
Imaging Flow Cytometry ◽  
Stimulated Raman

Combining the strength of flow cytometry with fluorescence imaging and digital image analysis, imaging flow cytometry is a powerful tool in diverse fields including cancer biology, immunology, drug discovery, microbiology, and metabolic engineering. It enables measurements and statistical analyses of chemical, structural, and morphological phenotypes of numerous living cells to provide systematic insights into biological processes. However, its utility is constrained by its requirement of fluorescent labeling for phenotyping. Here we present label-free chemical imaging flow cytometry to overcome the issue. It builds on a pulse pair-resolved wavelength-switchable Stokes laser for the fastest-to-date multicolor stimulated Raman scattering (SRS) microscopy of fast-flowing cells on a 3D acoustic focusing microfluidic chip, enabling an unprecedented throughput of up to ∼140 cells/s. To show its broad utility, we use the SRS imaging flow cytometry with the aid of deep learning to study the metabolic heterogeneity of microalgal cells and perform marker-free cancer detection in blood.

scholarly journals Chemical imaging of live fibroblasts by SERS effective nanofilm

2014 ◽  
Vol 16 (44) ◽  
pp. 24621-24634 ◽  
Author(s):  
D. Radziuk ◽  
R. Schuetz ◽  
A. Masic ◽  
H. Moehwald
Keyword(s):  
Real Time ◽  
Chemical Imaging ◽  
Raman Imaging ◽  
Label Free

Label-free Raman imaging of live single NIH3T3 fibroblast produced by SERS effective SiO2@Ag–PAA nanoshells in real time.

scholarly journals High resolution mass spectrometry imaging of plant tissues: towards a plant metabolite atlas

The Analyst ◽  
2015 ◽  
Vol 140 (22) ◽  
pp. 7696-7709 ◽  
Author(s):  
Dhaka Ram Bhandari ◽  
Qing Wang ◽  
Wolfgang Friedt ◽  
Bernhard Spengler ◽  
Sven Gottwald ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
High Spatial Resolution ◽  
High Resolution Mass Spectrometry ◽  
Chemical Imaging ◽  
Plant Tissues ◽  
Label Free ◽  
Wide Range ◽  
Plant Metabolite ◽  
Resolution Mass

Label-free chemical imaging of a wide range of metabolites in all major plant organs acquired at high spatial resolution.

scholarly journals Inside Cover: Label-Free Chemical Imaging of Catalytic Solids by Coherent Anti-Stokes Raman Scattering and Synchrotron-Based Infrared Microscopy (Angew. Chem. Int. Ed. 47/2009)

2009 ◽  
Vol 48 (47) ◽  
pp. 8798-8798
Author(s):  
Marianne���H.���F. Kox ◽  
Katrin���F. Domke ◽  
James���P.���R. Day ◽  
Gianluca Rago ◽  
Eli Stavitski ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Chemical Imaging ◽  
Label Free ◽  
Infrared Microscopy ◽  
Anti Stokes

Label-Free Chemical Imaging of Latent Fingerprints with Stimulated Raman Scattering Microscopy

2017 ◽  
Vol 89 (8) ◽  
pp. 4468-4473 ◽  
Author(s):  
Benjamin Figueroa ◽  
Yikai Chen ◽  
Kyla Berry ◽  
Andrew Francis ◽  
Dan Fu
Keyword(s):  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Chemical Imaging ◽  
Label Free ◽  
Latent Fingerprints ◽  
Stimulated Raman
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