Extraction and separation of proteins by ionic liquid aqueous two-phase system

The Analyst ◽  
2013 ◽  
Vol 138 (21) ◽  
pp. 6445 ◽  
Author(s):  
Xiao Lin ◽  
Yuzhi Wang ◽  
Qun Zeng ◽  
Xueqin Ding ◽  
Jing Chen
Keyword(s):  
Ionic Liquid ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Extraction And Separation ◽  
Separation Of Proteins

scholarly journals Partitioning of Cephalexin in Ionic Liquid Aqueous Two-Phase System Composed of 1-Butyl-3-Methylimidazolium Tetrafluoroborate andZnSO4

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Yan Fang Li ◽  
Juan Han ◽  
Yun Wang ◽  
Jing Jing Ma ◽  
Yong Sheng Yan
Keyword(s):  
Ionic Liquid ◽  
Extraction Efficiency ◽  
Phase System ◽  
Two Phase ◽  
Rich Phase ◽  
Aqueous Two Phase System ◽  
Extraction And Separation ◽  
Partitioning Behavior

Ionic liquid aqueous two-phase system (ILATPS) was applied in the extraction and separation of hydrosoluble antibiotics. The partitioning behavior of cephalexin (CEX) in 1-butyl-3-methylimidazolium tetrafluoroborate ([Bmim]BF4)-ZnSO4aqueous two-phase system was studied by the partitioning parameter of the extraction efficiency. The effect of the volume of [Bmim]BF4, the concentration of ZnSO4, temperature, pH, and the volume of ZnSO4solution was discussed concretely. When the volume of [Bmim]BF4was 2 mL and the concentration of ZnSO4was 35%, the extraction efficiency of CEX could reach 92.64% with pH unadjusted. The effect of the volume of [Bmim]BF4on the extraction efficiency was higher than that of the concentration of ZnSO4. The temperature influenced not only the formation of aqueous two-phase system but also the extraction efficiency of CEX. The target was found to be preferentially extracted to the [Bmim]BF4-rich phase at the pH below 4.3. The partition of CEX to the top phase was enhanced by increasing the volume of [Bmim]BF4, the concentration of ZnSO4, and temperature; however, the partition of CEX to the top phase increased by decreasing the pH.

scholarly journals Extraction of Lysozyme by Hydroxyl-Functionalized Ionic Liquid Aqueous Two-Phase System

2015 ◽  
Vol 27 (8) ◽  
pp. 2759-2762 ◽  
Author(s):  
Bo Zhou ◽  
Gaozhang Gou ◽  
Shijuan Xu ◽  
Pei Zhang ◽  
Wei Liu
Keyword(s):  
Ionic Liquid ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Functionalized Ionic Liquid

scholarly journals Quantification of amino acids and peptides in an ionic liquid based aqueous two-phase system by LC–MS analysis

AMB Express ◽  
2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Sebastian Oppermann ◽  
Christina Oppermann ◽  
Miriam Böhm ◽  
Toni Kühl ◽  
Diana Imhof ◽  
...  
Keyword(s):  
Amino Acids ◽  
Ionic Liquid ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Ms Analysis

Aqueous two-phase system (polyethylene glycol + ionic liquid) for extraction of α-amylase: phase diagrams, systems characterization and partition study

2020 ◽  
Vol 37 (3) ◽  
pp. 595-606
Author(s):  
O. R. R. Gandolfi ◽  
G. R. F. Gonçalves ◽  
I. C. Batista ◽  
R. C. I. Fontan ◽  
C. M. Veloso ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Polyethylene Glycol ◽  
Phase Diagrams ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System
Sign in / Sign up

Export Citation Format

Share Document