Capture and separation of biomolecules using magnetic beads in a simple microfluidic channel without an external flow device

The Analyst ◽  
2013 ◽  
Vol 138 (21) ◽  
pp. 6573 ◽  
Author(s):  
Jingjing Wang ◽  
Kenneth Morabito ◽  
Tom Erkers ◽  
Anubhav Tripathi
Keyword(s):  
Magnetic Beads ◽  
Microfluidic Channel ◽  
External Flow ◽  
Flow Device

Magnetophoresis ‘meets’ viscoelasticity: deterministic separation of magnetic particles in a modular microfluidic device

Lab on a Chip ◽  
2015 ◽  
Vol 15 (8) ◽  
pp. 1912-1922 ◽  
Author(s):  
Francesco Del Giudice ◽  
Hojjat Madadi ◽  
Massimiliano M. Villone ◽  
Gaetano D'Avino ◽  
Angela M. Cusano ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Magnetic Particles ◽  
Magnetic Beads ◽  
Microfluidic Channel

Deflection of magnetic beads in a microfluidic channel can be improved through viscoelastic focusing.

scholarly journals Effective Isolation for Lung Carcinoma Cells Based on Immunomagnetic Separation in a Microfluidic Channel

Biosensors ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 23
Author(s):  
Hien Vu-Dinh ◽  
Hui Feng ◽  
Chun-Ping Jen
Keyword(s):  
Lung Cancer ◽  
Lung Carcinoma ◽  
Magnetic Beads ◽  
A549 Cells ◽  
Microfluidic Channel ◽  
Reference Method ◽  
Immunomagnetic Separation ◽  
Carcinoma Cells ◽  
Isolation System ◽  
Lung Carcinoma Cells

In this paper, we developed an isolation system for A549 human lung carcinoma cells as an effective factor for the early diagnosis of lung cancer. A microfluidic immunomagnetic method was used, in which the combination of immunomagnetic separation and a microfluidic system allowed for increased isolation efficiency with uncomplicated manipulation. In the microfluidic immunomagnetic strategy, A549 cells were combined with aptamer-conjugated carboxylated magnetic beads and then collected in a specified region by applying a magnetic field. The results were recorded using a fluorescence microscope, and the captured targets were then quantified. The isolation efficiency of A549 cells is up to 77.8%. This paper developed a simple working procedure, which is less time consuming, high-throughput, and trustworthy for the isolation of A549 cells. This procedure can be a useful reference method for the development of an effective diagnosis and treatment method for lung cancer in the future.

Magnetic and Electric Manipulation of a Single Cell in Fluid

2004 ◽  
Vol 820 ◽  
Author(s):  
Hakho Lee ◽  
Tom P. Hunt ◽  
Robert M. Westervelt
Keyword(s):  
Yeast Cell ◽  
Single Cell ◽  
Electric Fields ◽  
Magnetic Beads ◽  
Microfluidic Channel ◽  
Yeast Cells ◽  
Length Scales

AbstractMagnetic and electric manipulation of a single cell in a microfluidic channel was demonstrated using a microelectromagnet matrix and a micropost matrix. The microelectromagnet matrix is two perpendicular arrays of straight wires that are separated and topped by insulating layers. The micropost matrix is an array of post-shaped electrodes embedded in an insulting layer. By controlling the current in each wire of the microelectromagnet matrix or the voltage on each electrode of the micropost matrix, versatile magnetic or electric fields were created on micrometer length scales, controlling the motion of individual cells in fluid. Single or multiple yeast cells attached to magnetic beads were trapped and moved by the microelectromagnet matrix; a single yeast cell was directly trapped and moved by the micropost matrix.

scholarly journals Microfluidic magnetic bead conveyor belt

Lab on a Chip ◽  
2017 ◽  
Vol 17 (22) ◽  
pp. 3826-3840 ◽  
Author(s):  
Stijn van Pelt ◽  
Arjan Frijns ◽  
Jaap den Toonder
Keyword(s):  
Magnetic Beads ◽  
Microfluidic Channel ◽  
Magnetic Bead ◽  
Conveyor Belt ◽  
Soft Magnetic ◽  
Magnetic Structures ◽  
On Chip ◽  
Rotating Field

Magnetic beads can be manipulated in a microfluidic channel using on-chip soft-magnetic structures and an external rotating field.

Improved micro-impedance spectroscopy to determine cell barrier properties

2020 ◽  
Vol 4 (3) ◽  
pp. 150-155 ◽  
Author(s):  
Md. Mehadi Hasan Sohag ◽  
Olivier Nicoud ◽  
Racha Amine ◽  
Abir Khalil-Mgharbel ◽  
Jean-Pierre Alcaraz ◽  
...  
Keyword(s):  
Impedance Spectroscopy ◽  
Epithelial Cells ◽  
Lipid Bilayers ◽  
Cultured Cells ◽  
Cell Model ◽  
Measurement Techniques ◽  
Cell Monolayer ◽  
Microfluidic Channel ◽  
Small Surface ◽  
Resistance Pathway

AbstractThe goal of this study was to determine whether the Tethapod system, which was designed to determine the impedance properties of lipid bilayers, could be used for cell culture in order to utilise micro-impedance spectroscopy to examine further biological applications. To that purpose we have used normal epithelial cells from kidney (RPTEC) and a kidney cancer cell model (786-O). We demonstrate that the Tethapod system is compatible with the culture of 10,000 cells seeded to grow on a small area gold measurement electrode for several days without affecting the cell viability. Furthermore, the range of frequencies for EIS measurements were tuned to examine easily the characteristics of the cell monolayer. We demonstrate significant differences in the paracellular resistance pathway between normal and cancer kidney epithelial cells. Thus, we conclude that this device has advantages for the study of cultured cells that include (i) the configuration of measurement and reference electrodes across a microfluidic channel, and (ii) the small surface area of 6 parallel measurement electrodes (2.1 mm2) integrated in a microfluidic system. These characteristics might improve micro-impedance spectroscopy measurement techniques to provide a simple tool for further studies in the field of the patho-physiology of biological barriers.

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