A novel aptasensor for the ultra-sensitive detection of adenosine triphosphate via aptamer/quantum dot based resonance energy transfer

The Analyst ◽  
2013 ◽  
Vol 138 (17) ◽  
pp. 4732 ◽  
Author(s):  
Zheng Li ◽  
Yijing Wang ◽  
Ying Liu ◽  
Yongyi Zeng ◽  
Aimin Huang ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Quantum Dot ◽  
Adenosine Triphosphate ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Sensitive Detection

Terbium-to-quantum dot Förster resonance energy transfer for homogeneous and sensitive detection of histone methyltransferase activity

Nanoscale ◽  
2020 ◽  
Vol 12 (25) ◽  
pp. 13719-13730
Author(s):  
Tooba Hallaj ◽  
Mohammad Amjadi ◽  
Xue Qiu ◽  
Kimihiro Susumu ◽  
Igor L. Medintz ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Quantum Dot ◽  
Enzyme Kinetics ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Histone Methyltransferase ◽  
Sensitive Detection ◽  
Förster Resonance Energy Transfer ◽  
Methyltransferase Activity ◽  
Inhibition Constants

Terbium-to-quantum dot FRET assays for quantifying SET7/9 methyltransferase at low picomolar concentrations, inhibition constants, and enzyme kinetics.

Single quantum dot-based nanosensor for rapid and sensitive detection of terminal deoxynucleotidyl transferase

2017 ◽  
Vol 53 (80) ◽  
pp. 11016-11019 ◽  
Author(s):  
Li-Juan Wang ◽  
Ming-Li Luo ◽  
Qianyi Zhang ◽  
Bo Tang ◽  
Chun-Yang Zhang
Keyword(s):  
Energy Transfer ◽  
Quantum Dot ◽  
Fluorescence Resonance Energy Transfer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Sensitive Detection ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Single Quantum ◽  
Single Quantum Dot ◽  
Fluorescence Resonance

We developed a single quantum dot-based fluorescence resonance energy transfer nanosensor for rapid and sensitive detection of terminal deoxynucleotidyl transferase.

Quantum Dot Bioconjugates as Energy Donors in Fluorescence Resonance Energy Transfer Assays

2003 ◽  
Vol 773 ◽  
Author(s):  
Aaron R. Clapp ◽  
Igor L. Medintz ◽  
J. Matthew Mauro ◽  
Hedi Mattoussi
Keyword(s):  
Energy Transfer ◽  
Quantum Dot ◽  
Organic Dyes ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Genetically Engineered ◽  
Molar Ratio ◽  
Binding Assays ◽  
Specific Sequence ◽  
Donor Acceptor

AbstractLuminescent CdSe-ZnS core-shell quantum dot (QD) bioconjugates were used as energy donors in fluorescent resonance energy transfer (FRET) binding assays. The QDs were coated with saturating amounts of genetically engineered maltose binding protein (MBP) using a noncovalent immobilization process, and Cy3 organic dyes covalently attached at a specific sequence to MBP were used as energy acceptor molecules. Energy transfer efficiency was measured as a function of the MBP-Cy3/QD molar ratio for two different donor fluorescence emissions (different QD core sizes). Apparent donor-acceptor distances were determined from these FRET studies, and the measured distances are consistent with QD-protein conjugate dimensions previously determined from structural studies.

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