Polymethylmethacrylate end-group analysis by matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF-MS)

1995 ◽  
pp. 561 ◽  
Author(s):  
Darren R. Maloney ◽  
Kirsty H. Hunt ◽  
Paul M. Lloyd ◽  
Andrew V. G. Muir ◽  
Stuart N. Richards ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Group Analysis ◽  
Time Of Flight ◽  
Laser Desorption ◽  
Maldi Tof Ms ◽  
Flight Mass Spectrometry ◽  
End Group ◽  
Tof Ms ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

scholarly journals Matrix-assisted laser desorption/ionisation-time of flight mass spectrometry (MALDI-TOF MS) in clinical mircobiological routine diagnostics1)

2011 ◽  
Vol 35 (4) ◽  
pp. ---
Author(s):  
Sören Schubert ◽  
Andreas Wieser
Keyword(s):  
Mass Spectrometry ◽  
Time Of Flight ◽  
Laser Desorption ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Tract Infections ◽  
Tof Ms ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

Abstract Very recently a novel method for differentiation of bacteria and fungi was developed, that is, identification by means of matrix-assisted laser desorption/ionisation-time of flight mass spectrometry (MALDI-TOF MS). This differentiation relies on the exact measurement of species-specific protein spectra of ribosomal proteins. It is at least as accurate as conventional biochemical differentiation methods, but provides results within minutes. In addition to differentiation of bacteria and yeasts grown on agar plates, direct identification is feasible from positive blood cultures as well as from urine samples of patients suffering from urinary tract infections. Future developments of MALDI-TOF MS for clinical microbiological purposes include the detection of β-lactamase and carbapenemase activity as well as genotyping of bacteria below the species level.

scholarly journals Molecular and Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry-Based Characterization of Clinically Significant Melanized Fungi in India

2017 ◽  
Vol 55 (4) ◽  
pp. 1090-1103 ◽  
Author(s):  
Ashutosh Singh ◽  
Pradeep Kumar Singh ◽  
Anil Kumar ◽  
Jagdish Chander ◽  
Geetika Khanna ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Time Of Flight ◽  
Laser Desorption ◽  
Content Type ◽  
Maldi Tof Ms ◽  
Flight Mass Spectrometry ◽  
Melanized Fungi ◽  
Tof Ms ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

ABSTRACTMelanized or black fungi are a heterogeneous group of fungi causing cutaneous to systemic diseases with high mortality. These fungi are rarely reported as agents of human infections, primarily due to difficulties in their classical identification. In this study, we examined, using molecular methods and matrix-assisted laser desorption ioniazation–time of flight mass spectrometry (MALDI-TOF MS), the diversity of melanized fungi (MF) isolated from patients in 19 medical centers in India. Overall, during a 4-year period, 718 (5.3%) clinical specimens yielded MF. Of these, 72 (10%) isolates had clinical significance and were identified primarily by sequencing the internal transcribed spacer and large subunit (LSU) regions. MF represented 21 genera comprising 29 species, the majority of them belonging to the orders Pleosporales (50%) and Chaetothyriales (22%). Among the 29 fungal species identified in this study, only 6 (20%) species were identified by the MALDI-TOF MS due to the limited commercial database of Bruker Daltonics for MF. However, a 100% identification rate of 20 additional species identified in this study was obtained by constructing an in-house database using 24- to 96-h-old liquid cultures. Further, the CLSI broth microdilution method revealed low MICs for posaconazole (≤1 μg/ml) and voriconazole (≤2 μg/ml) in 96% and 95% of isolates, respectively. Skin/subcutaneous and sino-nasal and pulmonary phaeohyphomycosis due to MF were diagnosed in 21.4% (n= 15) and 28.5% (n= 20) of cases. Also, 10% of patients had central nervous system involvement (n= 7), and 3 cases of fungal osteomyelitis due toCladophialophora bantianaandCorynesporaspp. were observed.

Sign in / Sign up

Export Citation Format

Share Document