3,4-Dimethylindole-2-carboxylate and 4-(1-hydroxyethyl)quinoline-2-carboxylate activating enzymes from the nosiheptide and thiostrepton producers, Streptomyces actuosus and Streptomyces laurentii

1993 ◽  
pp. 1612 ◽  
Author(s):  
Todd M. Smith ◽  
Nigel D. Priestley ◽  
Andrew R. Knaggs ◽  
Tom Nguyen ◽  
Heinz G. Floss
Keyword(s):  
Streptomyces Actuosus

Genome Shuffling and Ribosome Engineering of Streptomyces actuosus for High-Yield Nosiheptide Production

2014 ◽  
Vol 173 (6) ◽  
pp. 1553-1563 ◽  
Author(s):  
Qingling Wang ◽  
Dong Zhang ◽  
Yudong Li ◽  
Fuming Zhang ◽  
Cao Wang ◽  
...  
Keyword(s):  
Genome Shuffling ◽  
High Yield ◽  
Ribosome Engineering ◽  
Streptomyces Actuosus

Nucleotide sequence and transcriptional analysis of the nosiheptide-resistance gene from Streptomyces actuosus

Gene ◽  
1990 ◽  
Vol 91 (1) ◽  
pp. 9-17 ◽  
Author(s):  
Yum Li ◽  
Donald C. Dosch ◽  
William R. Strohl ◽  
Heinz G. Floss
Keyword(s):  
Nucleotide Sequence ◽  
Resistance Gene ◽  
Transcriptional Analysis ◽  
Streptomyces Actuosus

scholarly journals Genome Mining-Mediated Discovery of a New Avermipeptin Analogue in Streptomyces actuosus ATCC 25421

ChemistryOpen ◽  
2018 ◽  
Vol 7 (7) ◽  
pp. 558-561 ◽  
Author(s):  
Weiying Liu ◽  
Fengxian Sun ◽  
Yang Hu
Keyword(s):  
Genome Mining ◽  
Streptomyces Actuosus

Identification of Nosiheptide in Feeds and Detection of Residues in Animal Tissues

1979 ◽  
Vol 62 (5) ◽  
pp. 976-981 ◽  
Author(s):  
Claude Pascal ◽  
Claude Gaillard ◽  
Marie-O Moreau
Keyword(s):  
Staphylococcus Aureus ◽  
Standard Deviation ◽  
Colorimetric Method ◽  
Feed Additive ◽  
Animal Tissues ◽  
Microbiological Method ◽  
Fermentation Broths ◽  
Layer Chromatography ◽  
Streptomyces Actuosus

Abstract Nosiheptide is determined in fermentation broths of Streptomyces actuosus either by a microbiological method using Staphylococcus aureus or, more easily, by an automated colorimetric method. The results obtained with both methods correspond well for concentrations greater than 100 μg/mL with a standard deviation of 1-3%. For determination of nosiheptide as a feed additive, the microbiological assay is made more specific by pretreatment with petroleum ether and IN HCL Standard deviation is <4%, and the assay is sensitive to 1 ppm. Nosiheptide is identified in feed containing other frequently used antibiotics by thin layer chromatography with bioautography; sensitivity is 1 ppm. The absence of traces of nosiheptide in tissues of treated swine and broiler is confirmed by microbiological diffusion, sensitive to 0.025 ppm.

Production of xylanases from rice bran by Streptomyces actuosus A-151

2003 ◽  
Vol 33 (7) ◽  
pp. 917-925 ◽  
Author(s):  
San-Lang Wang ◽  
Yue-Horng Yen ◽  
Ing-Lung Shih ◽  
Audrey Chingzu Chang ◽  
Wen-Teish Chang ◽  
...  
Keyword(s):  
Rice Bran ◽  
Streptomyces Actuosus

Biosynthesis of the modified peptide antibiotic nosiheptide in Streptomyces actuosus

1988 ◽  
Vol 110 (17) ◽  
pp. 5800-5806 ◽  
Author(s):  
David R. Houck ◽  
Li Chun. Chen ◽  
Paul J. Keller ◽  
John M. Beale ◽  
Heinz G. Floss
Keyword(s):  
Peptide Antibiotic ◽  
Streptomyces Actuosus

scholarly journals Crystallographic analysis of NosA, which catalyzes terminal amide formation in the biosynthesis of nosiheptide

2015 ◽  
Vol 71 (8) ◽  
pp. 1033-1037 ◽  
Author(s):  
Yanting Wang ◽  
Shanshan Liu ◽  
Pengfei Yao ◽  
Yi Yu ◽  
Yan Zhang ◽  
...  
Keyword(s):  
Synchrotron Radiation ◽  
Diffraction Data ◽  
Crystallographic Analysis ◽  
Diffusion Method ◽  
Asymmetric Unit ◽  
Unit Cell Parameters ◽  
Solvent Content ◽  
Cell Parameters ◽  
Vapour Diffusion ◽  
Streptomyces Actuosus

Nosiheptide is a member of the thiopeptide family of antibiotics which demonstrates potent activities against various bacterial pathogens. The formation of its C-terminal amide is catalysed by NosA in an unusual strategy for maturating certain thiopeptides by processing precursor peptides featuring a serine extension. Here, a recombinant C-terminally truncated selenomethionine-derivatized NosA1–111variant fromStreptomyces actuosusconsisting of residues 1–111, named SeMet NosA1–111, was crystallized using the sitting-drop vapour-diffusion method. Diffraction data were collected to 2.40 Å resolution using synchrotron radiation. The crystals belonged to the primitive cubic space groupP4132, with unit-cell parametersa=b=c= 143.3 Å. Assuming the presence of three molecules in the asymmetric unit, the calculated Matthews coefficient was 3.94 Å3 Da−1and the corresponding solvent content was 40.3%.

Sign in / Sign up

Export Citation Format

Share Document