The determination of the handedness of cholesteric superhelices formed by DNA fragments

1988 ◽  
pp. 953 ◽  
Author(s):  
Gian Piero Spada ◽  
Patrizia Brigidi ◽  
Giovanni Gottarelli
Keyword(s):  
Dna Fragments

scholarly journals Computer programs for accurate determination of size of DNA fragments in agarose gels.

1987 ◽  
Vol 40 (6) ◽  
pp. 692-695 ◽  
Author(s):  
J Ling ◽  
K L Ling ◽  
K W Chan ◽  
G L French
Keyword(s):  
Accurate Determination ◽  
Computer Programs ◽  
Dna Fragments ◽  
Agarose Gels

LC-EC determination of nucleotides and nucleic acids: Application to enzyme assays and the analysis of DNA fragments

1988 ◽  
Vol 169 (1) ◽  
pp. 121-131 ◽  
Author(s):  
David P. Malliaros ◽  
Mark J. DeBenedetto ◽  
Pamela M. Guy ◽  
Terrence P. Tougas ◽  
Edwin G.E. Jahngen
Keyword(s):  
Nucleic Acids ◽  
Enzyme Assays ◽  
Dna Fragments

A fast method for the determination of 5′ terminal dinucleotides of DNA fragments

1975 ◽  
Vol 64 (2) ◽  
pp. 321-328 ◽  
Author(s):  
Alberto Bernardi ◽  
Claire Gaillard
Keyword(s):  
Fast Method ◽  
Dna Fragments

scholarly journals Micro-determination of proteins and DNA-fragments by use of light scanning photoacoustic densitometry.

1988 ◽  
Vol 37 (3) ◽  
pp. 147-151
Author(s):  
Keiko OHSAWA ◽  
Katsumi UCHIYAMA ◽  
Shinichi SUZUMURA ◽  
Kazuo IMAEDA ◽  
Kohei TAMURA
Keyword(s):  
Dna Fragments

scholarly journals Construction of DNA ladder for determination of small size DNA fragments

2021 ◽  
Vol 19 (3) ◽  
pp. 539-545
Author(s):  
Vo Thi Thuong Lan ◽  
Le Thi Thanh
Keyword(s):  
Molecular Biology ◽  
Restriction Enzyme ◽  
Dna Markers ◽  
Dna Marker ◽  
Dna Fragments ◽  
Dna Ladder ◽  
Time Saving ◽  
Pcr Product ◽  
Dna Fragment

DNA marker is commonly used to determine the size of DNA fragments by electrophoresis in routine molecular biology laboratories. In this study, we report a new procedure to prepare recombinant plasmids pSY-60 which was partially digested by one restriction enzyme for generating DNA markers of 7 fragments from 60 to 420 bp. The procedure included a synthesis of 60 bp DNA fragment with EcoRI sites at both ends using PCR extension, self-ligation of the 60 bp fragments and subcloning the ligated product into plasmid, generating recombinant pSY-60. Once being cloned, 500 ng of 420 bp fragment purified from 100 µL PCR product was incompletely digested by EcoRI, sufficiently producing to 50 acrylamide gels. Our procedure for production of DNA markers could be simple, time saving and inexpensive in comparison with current ones widely used in most laboratories.

scholarly journals Fast size-determination of intact bacterial plasmids using nanofluidic channels

Lab on a Chip ◽  
2015 ◽  
Vol 15 (13) ◽  
pp. 2739-2743 ◽  
Author(s):  
K. Frykholm ◽  
L. K. Nyberg ◽  
E. Lagerstedt ◽  
C. Noble ◽  
J. Fritzsche ◽  
...  
Keyword(s):  
Dna Fragments ◽  
Size Determination ◽  
Linear Dna ◽  
Nanofluidic Channels ◽  
Bacterial Plasmids

We demonstrate how circular bacterial plasmids can be automatically sized and discriminated from linear DNA fragmentsviastretching in nanochannels.

Sign in / Sign up

Export Citation Format

Share Document