The role of leucine in isoprenoid metabolism. Incorporation of [3-13C]leucine and of [2-3H,4-14C]-β,β-dimethylacrylic acid into phytosterols by tissue cultures of Andrographis paniculata

1985 ◽  
pp. 148-149 ◽  
Author(s):  
Panayiotis Anastasis ◽  
Isabel Freer ◽  
Karl Overton ◽  
David Rycroft ◽  
Sheo Bux Singh
Keyword(s):  
Andrographis Paniculata ◽  
Tissue Cultures ◽  
Isoprenoid Metabolism

scholarly journals Comparison of different extraction kits to isolate microRNA fromGalleria mellonella(wax moth) larvae infected withMetarhizium brunneum(ARSEF4556)

2019 ◽  
Author(s):  
Muneefah A. Alenezi ◽  
Tariq M. Butt ◽  
Daniel C. Eastwood
Keyword(s):  
Fungal Pathogens ◽  
High Throughput Sequencing ◽  
Galleria Mellonella ◽  
Host Tissue ◽  
Tissue Cultures ◽  
Complex Environments ◽  
Infected Insect ◽  
Sequencing And Expression ◽  
Wax Moth

ABSTRACTMicroRNAs (miRNAs) play an important role in regulating gene expression and are involved in developmental processes in animals, plants and fungi. To understand the role of miRNAs in a biological system, it is important to optimise the extraction procedures to obtain high quality and quantity nucleic acid that enable high throughput sequencing and expression analysis. Numerous kit-based miRNA extraction protocols have been optimised generally to single cell or tissue cultures. Fungi, however, often occupy physically and chemically complex environments which miRNA make extraction challenging, such as fungal pathogens interacting within plant or animal host tissue. We used aGalleria mellonella(wax moth) larvae and entomopathogenic fungusMetarhizium brunneum ARSEF 4556host/pathogen model to compare commercially available miRNA extraction kits (Invitrogen PureLink™ miRNA Isolation Kit, Ambion mirVana™miRNA Isolation Kit and Norgen microRNA purification Kit). Our results showed reproducible and significant differences in miRNAs extraction between the kits, with the Invitrogen PureLink™ miRNA Isolation protocol demonstrating the best performance in terms of miRNA quantity, quality and integrity isolated from fungus-infected insect tissue.

Plant Cell and Tissue Cultures: The Role of Haberlandt

2003 ◽  
pp. 25-53 ◽  
Author(s):  
A. D. Krikorian ◽  
David L. Berquam
Keyword(s):  
Plant Cell ◽  
Tissue Cultures

scholarly journals Role of semi-purified andrographolide from Andrographis paniculata extract as nano-phytovesicular carrier for enhancing oral absorption and hypoglycemic activity

2020 ◽  
Vol 12 (2) ◽  
pp. 142-155
Author(s):  
Vinod Kumar Verma ◽  
Md. Kamaruz Zaman ◽  
Shekhar Verma ◽  
Santosh Kumar Verma ◽  
Khomendra Kumar Sarwa
Keyword(s):  
Oral Absorption ◽  
Hypoglycemic Activity ◽  
Andrographis Paniculata

scholarly journals THE LOCALIZATION OF SUCCINIC DEHYDROGENASE, CYTOCHROME OXIDASE AND ADENOSINE TRIPHOSPHATASE IN CILIATED RESPIRATORY EPITHELIUM

1965 ◽  
Vol 13 (8) ◽  
pp. 677-683 ◽  
Author(s):  
HERTHA R. CRESS ◽  
ALEXANDER SPOCK ◽  
DUNCAN C. HEATHERINGTON
Keyword(s):  
Cytochrome Oxidase ◽  
Adenosine Triphosphatase ◽  
Succinic Dehydrogenase ◽  
Respiratory Epithelium ◽  
Ciliary Activity ◽  
Basal Bodies ◽  
Tissue Cultures ◽  
Respiratory Epithelial Cells ◽  
Respiratory Epithelial

Succinic dehydrogenase and cytochrome oxidase activities were found to be scattered throughout the cytoplasm of ciliated and nonciliated respiratory epithelial cells while ATPase activity was restricted to cilia and areas under the cilia in the regions of the ciliary basal bodies. In order to elucidate the role of ATPase further, tissue cultures of rabbit tracheal epithelium with beating cilia were incubated in a medium perfused with cigarette smoke which resulted in cessation of ciliary motility. Epithelium with beating cilia was positive for ATPase while the epithelium with nonbeating cilia was negative or only weakly positive in a few small scattered areas. The presence of ATPase in beating cilia and its absence in nonbeating cilia agree with biochemical and physiological studies suggesting an association between ATP and ciliary activity.

Altering potato isoprenoid metabolism increases biomass and induces early flowering

2020 ◽  
Vol 71 (14) ◽  
pp. 4109-4124
Author(s):  
Moehninsi ◽  
Iris Lange ◽  
B Markus Lange ◽  
Duroy A Navarre
Keyword(s):  
Metabolic Regulation ◽  
Transcriptional Control ◽  
Mevalonate Pathway ◽  
Early Flowering ◽  
Bioenergy Crops ◽  
Isoprenoid Metabolism ◽  
Transgenic Lines ◽  
Coa Reductase ◽  
Rate Limiting

Abstract Isoprenoids constitute the largest class of plant natural products and have diverse biological functions including in plant growth and development. In potato (Solanum tuberosum), the regulatory mechanism underlying the biosynthesis of isoprenoids through the mevalonate pathway is unclear. We assessed the role of 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) homologs in potato development and in the metabolic regulation of isoprenoid biosynthesis by generating transgenic lines with down-regulated expression (RNAi-hmgr) or overexpression (OE) of one (StHMGR1 or StHMGR3) or two genes, HMGR and farnesyl diphosphate synthase (FPS; StHMGR1/StFPS1 or StHMGR3/StFPS1). Levels of sterols, steroidal glycoalkaloids (SGAs), and plastidial isoprenoids were elevated in the OE-HMGR1, OE-HMGR1/FPS1, and OE-HMGR3/FPS1 lines, and these plants exhibited early flowering, increased stem height, increased biomass, and increased total tuber weight. However, OE-HMGR3 lines showed dwarfism and had the highest sterol amounts, but without an increase in SGA levels, supporting a rate-limiting role for HMGR3 in the accumulation of sterols. Potato RNAi-hmgr lines showed inhibited growth and reduced cytosolic isoprenoid levels. We also determined the relative importance of transcriptional control at regulatory points of isoprenoid precursor biosynthesis by assessing gene–metabolite correlations. These findings provide novel insights into specific end-products of the sterol pathway and could be important for crop yield and bioenergy crops.

scholarly journals THE ROLE OF METHIONINE DEFICIENCY IN POLIOVIRUS REPLICATION IN TISSUE CULTURES

1966 ◽  
Vol 123 (1) ◽  
pp. 17-24 ◽  
Author(s):  
Soussan Mohajer ◽  
Janis Gabliks
Keyword(s):  
Amino Acids ◽  
Hela Cells ◽  
Inhibitory Effect ◽  
Monkey Kidney ◽  
Kidney Cells ◽  
Tissue Cultures ◽  
Experimental Conditions ◽  
Methionine Deficiency ◽  
Intracellular Amino Acids

The role of methionine in poliovirus infection in HeLa and monkey kidney cells was investigated by using the methionine analogue l-ethionine. In the presence of 2.0 x 10–3 and 4.0 x 10–3 moles ethionine, the growth of HeLa and monkey kidney cells was significantly inhibited. Under the same experimental conditions, ethionine had no significant effect on the biosynthesis of two strains of poliovirus (Mahoney and Lansing) in HeLa cells, whereas in primary monkey kidney cells, it markedly inhibited the biosynthesis of the Lansing strain of poliovirus. HeLa cells partly depleted of their intracellular amino acids did not change the rate of viral biosynthesis. The inhibitory effect of ethionine on cell growth and viral biosynthesis was reversed by addition of an excess of l-methionine.

Sign in / Sign up

Export Citation Format

Share Document