Mechanism of pyridoxal phosphate-dependent enzymatic amino-acid racemization

1983 ◽  
pp. 82 ◽  
Author(s):  
Shu-jane Shen ◽  
Heinz G. Floss ◽  
Hidehiko Kumagai ◽  
Hideaki Yamada ◽  
Nobuyoshi Esaki Kenji Soda ◽  
...  
Keyword(s):  
Amino Acid ◽  
Pyridoxal Phosphate ◽  
Amino Acid Racemization

scholarly journals A new method for enamel amino acid racemization dating: A closed system approach

2019 ◽  
Vol 50 ◽  
pp. 29-46 ◽  
Author(s):  
Marc R. Dickinson ◽  
Adrian M. Lister ◽  
Kirsty E.H. Penkman
Keyword(s):  
Amino Acid ◽  
Closed System ◽  
System Approach ◽  
New Method ◽  
Amino Acid Racemization

scholarly journals Effect of acid hydrolysis on the racemization method using amino acid racemization in teeth.

1988 ◽  
Vol 37 (9) ◽  
pp. 476-480 ◽  
Author(s):  
Tsuneko OGINO ◽  
Hideki OGIUCHI ◽  
Toshiyuki HOBO ◽  
Hiroshi OGINO
Keyword(s):  
Amino Acid ◽  
Acid Hydrolysis ◽  
Amino Acid Racemization

scholarly journals Specificity and properties of the nucleotide carrier in chromaffin granules from bovine adrenal medulla

1983 ◽  
Vol 210 (3) ◽  
pp. 789-794 ◽  
Author(s):  
A Weber ◽  
E W Westhead ◽  
H Winkler
Keyword(s):  
Amino Acid ◽  
Carboxylic Acid ◽  
Pyridoxal Phosphate ◽  
Chromaffin Granules ◽  
Inhibitory Effects ◽  
Temperature Dependent ◽  
Arginine Residues ◽  
Disulphonic Acid ◽  
Important Position ◽  
Broad Specificity

1. The influence of various substances on the uptake of [3H]ATP and [14C]-noradrenaline into isolated bovine chromaffin granules was investigated. The carrier-mediated [3H]ATP uptake is specifically inhibited by SO42-, PO43- and phosphoenolpyruvate. Compounds with carboxylic acid or sulphonic acid groups had no significant inhibitory effects on either uptake. 2. 35SO42-, 32PO43- and phosphoenol[14C]pyruvate are taken up into chromaffin granules by a temperature-dependent process that is inhibited by atractyloside, uncouplers of oxidative phosphorylation and lipid-permeant anions. The apparent Km of 35SO42- uptake is 0.4 mM. 3. These results indicate that the nucleotide carrier in chromaffin granules has a broad specificity, transporting compounds with two strong negative charges. 4. Amino acid probes influence the uptake of ATP and catecholamines differently. Pyridoxal phosphate inhibits both uptake processes, 4,4′-di-isothiocyanostilbene-2,2′-disulphonic acid preferentially blocks ATP uptake, whereas phenylglyoxal blocks only ATP transport. It is suggested that the nucleotide carrier possesses arginine residues in a functionally important position. 5. The significance of these results obtained on isolated granules for the function of chromaffin granules within the cell is discussed.

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