Folic acid-modified clay: targeted surface design for cell culture applications

2013 ◽  
Vol 1 (4) ◽  
pp. 522-528 ◽  
Author(s):  
Rebecca Bongartz ◽  
Didem Ag ◽  
Muharrem Seleci ◽  
Johanna-G. Walter ◽  
Esra E. Yalcinkaya ◽  
...  
Keyword(s):  
Cell Culture ◽  
Folic Acid ◽  
Surface Design ◽  
Modified Clay

scholarly journals Folic acid modified clay/polymer nanocomposites for selective cell adhesion

2014 ◽  
Vol 2 (37) ◽  
pp. 6412-6421 ◽  
Author(s):  
F. B. Barlas ◽  
D. Ag Seleci ◽  
M. Ozkan ◽  
B. Demir ◽  
M. Seleci ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Adhesion ◽  
Folic Acid ◽  
Polymer Nanocomposites ◽  
Promising Material ◽  
Modified Clay ◽  
A Cell ◽  
Cell Adhesion And Proliferation

A promising material, a folic acid modified poly(epsilon-caprolactone)/clay nanocomposite that allows selective cell adhesion and proliferation, was synthesized and characterized as a cell culture and biosensing platform.

scholarly journals The increase in receptor-mediated endocytosis of drugs in the composition of nanoparticles with the address fragment

2016 ◽  
Vol 62 (3) ◽  
pp. 306-310 ◽  
Author(s):  
L.V. Kostryukova ◽  
M.A. Sanzhakov ◽  
D.V. Ignatov ◽  
V.N. Prozorovskyi ◽  
O.S. Druzhilovskaya ◽  
...  
Keyword(s):  
Cell Culture ◽  
Folic Acid ◽  
Colloidal Nanoparticles ◽  
High Toxicity ◽  
Hep G2 ◽  
Modern Approach ◽  
Affected Area ◽  
Chlorine E6 ◽  
The Impact ◽  
Pharmaceutical Composition

It is known that disorders in the cell functioning of the organs/tissues is accompanied by increased expression of certain receptors. A modern approach to improve the specificity of the drug accumulation in the affected area is to construct the delivery nanosystems with the address fragments. Active tagged transport may help to reduce the dose of the drug, minimizing the impact on healthy cells and organs (reduced adverse events). This approach is particularly important in oncology because of the high toxicity of the drugs used. In this work we have obtained and characterized the pharmaceutical composition of doxorubicin and chlorine e6 into colloidal nanoparticles with synthesized previously targeted conjugates based on folic acid and biotin. On the cell culture Hep G2 it was shown an increase in the internalization of drugs when they were introduced in the incubation medium in the form of drug compositions with transport nanosystems and targeted fragments.

Dietary folic acid, uterine function and early embryonic development in sows

1996 ◽  
Vol 76 (3) ◽  
pp. 427-433 ◽  
Author(s):  
J. J. Matte ◽  
C. Farmer ◽  
C. L. Girard ◽  
J.-P. Laforest
Keyword(s):  
Cell Culture ◽  
Folic Acid ◽  
Embryonic Development ◽  
Total Protein Content ◽  
Experimental Diet ◽  
Early Gestation ◽  
Key Words Folic Acid ◽  
Uterine Environment ◽  
Estradiol 17Β

The present study was designed to determine the role of folic acid on uterine environment and embryonic development during early gestation in the pig. Thirty-two, third parity, crossbred sows received a diet supplemented with 0 or 15 mg kg−1 of folic acid. The treatments started 2 wk before expected estrus and lasted until slaughter on either day 12 or 15 after mating. One uterine horn was used to collect conceptuses and uterine "flushings" for hormonal and metabolite determinations; conceptuses from the other horn were enzymatically dispersed and placed in cell culture with and without dehydroepiandrosterone (DHEA). The decrease in serum folates was attenuated (P ≤ 0.06) and the total and saturated folate binding capacities in early gestation were increased (P < 0.01) in sows receiving additional dietary folic acid. The volume of uterine flushings recovered was greater (P ≤ 0.02) on day 15 than on day 12, as was its content of protein (P ≤ 0.06). In sows receiving the dietary supplement of folic acid, total uterine prostaglandin (PG)E2 was three times higher on day 12 and two times higher on day 15 (P < 0.04) than for sows fed the experimental diet without supplement; although numerically substantial (60% higher), the effect was not significant for PGF2α (P ≥ 0.16). Conceptus homogenates contained more folic acid (P ≤ 0.02) and DNA (P ≤ 0.0001) on day 15 than on day 12. Their total protein content, in sows slaughtered on day 12 of gestation, tended (P ≤ 0.07) to be higher in supplemented than in unsupplemented animals. The synthesis of estradiol-17β by the conceptus cells, used as an index of embryonic maturity, tended (P ≤ 0.07) to be lower for treated than untreated sows, especially in conceptus cell culture without DHEA. Therefore, the improvement in embryonic survival attributed to dietary supplements of folic acid might be linked to changes on the secretion of uterine prostaglandins and possibly on embryonic development. Key words: Folic acid, uterus, embryo, sow

Surface design of antibody-immobilized thermoresponsive cell culture dishes for recovering intact cells by low-temperature treatment

2013 ◽  
Vol 102 (11) ◽  
pp. 3883-3893 ◽  
Author(s):  
Jun Kobayashi ◽  
Masaki Hayashi ◽  
Takahiro Ohno ◽  
Masanori Nishi ◽  
Yoshinori Arisaka ◽  
...  
Keyword(s):  
Cell Culture ◽  
Low Temperature ◽  
Temperature Treatment ◽  
Intact Cells ◽  
Surface Design ◽  
Low Temperature Treatment

The Morphology of the Rat Kidney Following Folic Acid Administration

1970 ◽  
Vol 28 ◽  
pp. 200-201
Author(s):  
Aline Byrnes ◽  
Elsa E. Ramos ◽  
Minoru Suzuki ◽  
E.D. Mayfield
Keyword(s):  
Folic Acid ◽  
De Novo ◽  
Specific Activity ◽  
Rat Kidney ◽  
Present Report ◽  
Intracellular Localization ◽  
Male Rats ◽  
Renal Hypertrophy ◽  
Electron Microscopic ◽  
Biochemical Alterations

Renal hypertrophy was induced in 100 g male rats by the injection of 250 mg folic acid (FA) dissolved in 0.3 M NaHCO3/kg body weight (i.v.). Preliminary studies of the biochemical alterations in ribonucleic acid (RNA) metabolism of the renal tissue have been reported recently (1). They are: RNA content and concentration, orotic acid-c14 incorporation into RNA and acid soluble nucleotide pool, intracellular localization of the newly synthesized RNA, and the specific activity of enzymes of the de novo pyrimidine biosynthesis pathway. The present report describes the light and electron microscopic observations in these animals. For light microscopy, kidney slices were fixed in formalin, embedded, sectioned, and stained with H & E and PAS.

A T.E.M. study of mouse mammary epithelial cell secretory differentiation cultured on collagen and Matrigel

1991 ◽  
Vol 49 ◽  
pp. 188-189
Author(s):  
W.N. Bentham ◽  
V. Rocha
Keyword(s):  
Cell Culture ◽  
Mammary Epithelial ◽  
Secretory Process ◽  
Cell Culture System ◽  
Protein Maturation ◽  
Cell Culture Techniques ◽  
Culture Techniques ◽  
Mouse Mammary Epithelial Cell ◽  
Secretory Differentiation

It has been an interest of our lab to develop a mammary epethelial cell culture system that faithfully duplicates the in vivo condition of the lactating gland. Since the introduction of collagen as a matrix on which cells are cultivated other E.C.M. type matrices have been made available and are used in many cell culture techniques. We have previously demonstrated that cells cultured on collagen and Matrigel do not differentiate as they do in vivo. It seems that these cultures often produce cells that show a disruption in the secretory process. The appearance of large ribosomal studded vesicles, that specifically label with antibody to casein, suggest an interruption of both protein maturation and secretion at the E.R. to golgi transition. In this report we have examined cultures on collagen and Matrigel at relative high and low seeding densities and compared them to cells from the in vivo condition.

Solid-phase immunoelectron microscopy for rapid diagnosis of enteroviruses

1984 ◽  
Vol 42 ◽  
pp. 226-227 ◽  
Author(s):  
K. Pegg-Feige ◽  
F. W. Doane
Keyword(s):  
Electron Microscopy ◽  
Cell Culture ◽  
Immunoelectron Microscopy ◽  
Detection Method ◽  
Solid Phase ◽  
Protein A ◽  
Plant Viruses ◽  
Rapid Diagnosis ◽  
Virus Diagnosis ◽  
Antiviral Antibody

Immunoelectron microscopy (IEM) applied to rapid virus diagnosis offers a more sensitive detection method than direct electron microscopy (DEM), and can also be used to serotype viruses. One of several IEM techniques is that introduced by Derrick in 1972, in which antiviral antibody is attached to the support film of an EM specimen grid. Originally developed for plant viruses, it has recently been applied to several animal viruses, especially rotaviruses. We have investigated the use of this solid phase IEM technique (SPIEM) in detecting and identifying enteroviruses (in the form of crude cell culture isolates), and have compared it with a modified “SPIEM-SPA” method in which grids are coated with protein A from Staphylococcus aureus prior to exposure to antiserum.

Automated cell counting of astrocytes on patterned substrates containing aliphatic and charged properties

1995 ◽  
Vol 53 ◽  
pp. 974-975
Author(s):  
W. Shain ◽  
H. Ancin ◽  
H.C. Craighead ◽  
M. Isaacson ◽  
L. Kam ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Attachment ◽  
Culture Method ◽  
Cell Counting ◽  
Data Sets ◽  
Nuclear Staining ◽  
Double Positive ◽  
A Cell ◽  
Wafer Test ◽  
Cell Densities

Neural protheses have potential to restore nervous system functions lost by trauma or disease. Nanofabrication extends this approach to implants for stimulating and recording from single or small groups of neurons in the spinal cord and brain; however, tissue compatibility is a major limitation to their practical application. We are using a cell culture method for quantitatively measuring cell attachment to surfaces designed for nanofabricated neural prostheses.Silicon wafer test surfaces composed of 50-μm bars separated by aliphatic regions were fabricated using methods similar to a procedure described by Kleinfeld et al. Test surfaces contained either a single or double positive charge/residue. Cyanine dyes (diIC18(3)) stained the background and cell membranes (Fig 1); however, identification of individual cells at higher densities was difficult (Fig 2). Nuclear staining with acriflavine allowed discrimination of individual cells and permitted automated counting of nuclei using 3-D data sets from the confocal microscope (Fig 3). For cell attachment assays, LRM5 5 astroglial cells and astrocytes in primary cell culture were plated at increasing cell densities on test substrates, incubated for 24 hr, fixed, stained, mounted on coverslips, and imaged with a 10x objective.

Sign in / Sign up

Export Citation Format

Share Document