Highly stable quantum dots with silica–poly(EGDMA-co-MAA) synergistic protection and the preliminary application in immunoassay

2013 ◽  
Vol 1 (8) ◽  
pp. 1156 ◽  
Author(s):  
Junqing Liu ◽  
Tao Song ◽  
Qiuhua Yang ◽  
Jian Tan ◽  
Dinghai Huang ◽  
...  
Molecules ◽  
2021 ◽  
Vol 26 (16) ◽  
pp. 4994
Author(s):  
Weitao Li ◽  
Ningjia Jiang ◽  
Bin Wu ◽  
Yuan Liu ◽  
Luoman Zhang ◽  
...  

Biological imaging is an essential means of disease diagnosis. However, semiconductor quantum dots that are used in bioimaging applications comprise toxic metal elements that are nonbiodegradable, causing serious environmental problems. Herein, we developed a novel ecofriendly solvothermal method that uses ethanol as a solvent and doping with chlorine atoms to prepare highly fluorescent graphene quantum dots (GQDs) from seaweed. The GQDs doped with chlorine atoms exhibit high-intensity white fluorescence. Thus, their preliminary application in bioimaging has been confirmed. In addition, clear cell imaging could be performed at an excitation wavelength of 633 nm.


Author(s):  
Ruda Xu ◽  
Qiao Chen ◽  
Min Xia ◽  
Bing Bai ◽  
Yuemei Li ◽  
...  

Efficient utilization of quantum dots (QDs) in bulk-scale matrix with good enough performance but small quantity is important in real industrial applications. Including the concern of traditional problems of QDs,...


Blood ◽  
2008 ◽  
Vol 112 (11) ◽  
pp. 4908-4908
Author(s):  
Veena Kapoor ◽  
Fran Hakim ◽  
Najibah Rehman ◽  
Ronald E Gress ◽  
William G Telford

Abstract The Flow-FISH technique used to measure telomere length by incorporation of fluorescein-labeled oligonucleotide probes complementary to telomeres repeat sequences has been extended to permit simultaneous multiple phenotypic analysis within a single sample. The necessary thermal stability of the fluorescent labeling needed for phenotyping cell populations can be achieved using quantum dots instead of conventional organic fluorophores, which are usually damaged during the high-temperature hybridization treatment and lose their fluorescence, limiting their usefulness for phenotypic analysis. In this study, quantum dots and conventional fluorophores were compared for their ability to survive the 82oC hybridization step necessary for PNA probe annealing. Quantum dots preserved their fluorescence following heat treatment and gave good signal-to-noise ratios when used to detect lymphocyte and monocyte markers (CD3, CD4 and CD14). This was in contrast to phycobiliproteins and low molecular weight fluorochromes such as fluorescein and the Alexa Fluor dyes, which decreased considerably in fluorescence following hybridization. Since addition of fluorescent immunophenotyping complicated an assay already heavily dependent on uniform technique, internal reference standards (previously reported by Lansdorp) were particularly critical in this technique. We used aliquoted frozen calf thymocytes (CT) as an internal standard, determining their telomere length and incorporating them into each sample to monitor tube to tube and day to day assay variability. The telomere length of the CTs and of CD3+CD4+, CD3+CD4− and CD14+, were determined and then corrected by normalization to the known CT telomere length. To validate the overall method, we then measured age dependence on telomere length for monocyte and lymphocyte subpopulations in young, middle-aged and older donors. Results were consistent with results previously reported using traditional Southern blotting and Flow-FISH with no immunphenotyping, suggesting that incorporation of immunolabeling did not adversely affect this technique. This method allows for the rapid, accurate and simultaneous determination of the telomere lengths of different cell populations within a single sample when little amount of sample is available.


2020 ◽  
Vol 12 (1) ◽  
Author(s):  
Jaewan Ko ◽  
Byeong Guk Jeong ◽  
Jun Hyuk Chang ◽  
Joonyoung F. Joung ◽  
Suk-Young Yoon ◽  
...  

2007 ◽  
Vol 18 (20) ◽  
pp. 205603 ◽  
Author(s):  
Haibing Li ◽  
Xiaoqiong Wang ◽  
Zhinong Gao ◽  
Zhike He

2017 ◽  
Vol 5 (19) ◽  
pp. 4629-4635 ◽  
Author(s):  
Ting Han ◽  
Ye Yuan ◽  
Xiao Liang ◽  
Yang Zhang ◽  
Chuanxi Xiong ◽  
...  

A new ligand was reported to effectively prepare water-dispersible QDs with remarkable colloidal stability for inkjet printing.


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