A three dimensional soft matter cell model for mechanotransduction

Soft Matter ◽  
2012 ◽  
Vol 8 (21) ◽  
pp. 5765 ◽  
Author(s):  
Xiaowei Zeng ◽  
Shaofan Li
Keyword(s):  
Soft Matter ◽  
Cell Model ◽  
Three Dimensional

scholarly journals Programming the shape-shifting of flat soft matter: from self-rolling/self-twisting materials to self-folding origami

2016 ◽  
Vol 3 (6) ◽  
pp. 536-547 ◽  
Author(s):  
S. Janbaz ◽  
R. Hedayati ◽  
A. A. Zadpoor
Keyword(s):  
Soft Matter ◽  
Three Dimensional ◽  
Complex Surface ◽  
Surface Features

Rational programming of the shape-shifting behavior of flat materials (possibly decorated with complex surface features) to create complex three-dimensional structures.

scholarly journals An optimised GAS-pharyngeal cell biofilm model

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Heema K. N. Vyas ◽  
Jason D. McArthur ◽  
Martina L. Sanderson-Smith
Keyword(s):  
Crystal Violet ◽  
Biofilm Formation ◽  
Cell Model ◽  
Matrix Material ◽  
Three Dimensional ◽  
Growth Period ◽  
Optimal Growth ◽  
Abiotic Surfaces

AbstractGroup A Streptococcus (GAS) causes 700 million infections and accounts for half a million deaths per year. Biofilm formation has been implicated in both pharyngeal and dermal GAS infections. In vitro, plate-based assays have shown that several GAS M-types form biofilms, and multiple GAS virulence factors have been linked to biofilm formation. Although the contributions of these plate-based studies have been valuable, most have failed to mimic the host environment, with many studies utilising abiotic surfaces. GAS is a human specific pathogen, and colonisation and subsequent biofilm formation is likely facilitated by distinct interactions with host tissue surfaces. As such, a host cell-GAS model has been optimised to support and grow GAS biofilms of a variety of GAS M-types. Improvements and adjustments to the crystal violet biofilm biomass assay have also been tailored to reproducibly detect delicate GAS biofilms. We propose 72 h as an optimal growth period for yielding detectable biofilm biomass. GAS biofilms formed are robust and durable, and can be reproducibly assessed via staining/washing intensive assays such as crystal violet with the aid of methanol fixation prior to staining. Lastly, SEM imaging of GAS biofilms formed by this model revealed GAS cocci chains arranged into three-dimensional aggregated structures with EPS matrix material. Taken together, we outline an efficacious GAS biofilm pharyngeal cell model that can support long-term GAS biofilm formation, with biofilms formed closely resembling those seen in vivo.

scholarly journals Using 3D epigenomic maps of primary olfactory neuronal cells from living individuals to understand gene regulation

2018 ◽  
Vol 4 (12) ◽  
pp. eaav8550 ◽  
Author(s):  
Suhn K. Rhie ◽  
Shannon Schreiner ◽  
Heather Witt ◽  
Chris Armoskus ◽  
Fides D. Lay ◽  
...  
Keyword(s):  
Cell Model ◽  
Expression Profiles ◽  
Neuronal Cells ◽  
Three Dimensional ◽  
Gene Expression Profiles ◽  
Regulatory Elements ◽  
A Genome ◽  
Wide Scale ◽  
Cell Model System ◽  
Distal Regulatory Elements

As part of PsychENCODE, we developed a three-dimensional (3D) epigenomic map of primary cultured neuronal cells derived from olfactory neuroepithelium (CNON). We mapped topologically associating domains and high-resolution chromatin interactions using Hi-C and identified regulatory elements using chromatin immunoprecipitation and nucleosome positioning assays. Using epigenomic datasets from biopsies of 63 living individuals, we found that epigenetic marks at distal regulatory elements are more variable than marks at proximal regulatory elements. By integrating genotype and metadata, we identified enhancers that have different levels corresponding to differences in genetic variation, gender, smoking, and schizophrenia. Motif searches revealed that many CNON enhancers are bound by neuronal-related transcription factors. Last, we combined 3D epigenomic maps and gene expression profiles to predict enhancer-target gene interactions on a genome-wide scale. This study not only provides a framework for understanding individual epigenetic variation using a primary cell model system but also contributes valuable data resources for epigenomic studies of neuronal epithelium.

Three-dimensional printing with sacrificial materials for soft matter manufacturing

MRS Bulletin ◽  
2017 ◽  
Vol 42 (08) ◽  
pp. 571-577 ◽  
Author(s):  
Christopher S. O’Bryan ◽  
Tapomoy Bhattacharjee ◽  
Sean R. Niemi ◽  
Sidhika Balachandar ◽  
Nicholas Baldwin ◽  
...  
Keyword(s):  
Soft Matter ◽  
Three Dimensional ◽  
Three Dimensional Printing ◽  
Image Position ◽  
Sacrificial Materials ◽  
Dimensional Printing

Abstract

A Three-Dimensional Lung Cell Model to Leptospira Virulence Investigations

2022 ◽  
Vol 79 (2) ◽  
Author(s):  
Camila L. Campos ◽  
Luciana R. Gomes ◽  
Ambart E. Covarrubias ◽  
Ellen E. Kato ◽  
Gisele G. Souza ◽  
...  
Keyword(s):  
Cell Model ◽  
Three Dimensional

scholarly journals Three-Dimensional Generalized Dynamics of Soft-Matter Quasicrystals

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Tian-You Fan ◽  
Zhi-Yi Tang
Keyword(s):  
Boundary Value Problems ◽  
Soft Matter ◽  
Boundary Value ◽  
Three Dimensional ◽  
Governing Equations

The three-dimensional generalized dynamics of soft-matter quasicrystals was investigated, in which the governing equations of the dynamics are derived for observed 12-fold symmetry quasicrystals and possibly observed 8- and 10-fold symmetry ones in soft matter. The solving methods, possible solutions for some initial- and boundary-value problems of the equations, and possible applications are discussed as well.

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