Size dependent cellular uptake, in vivo fate and light–heat conversion efficiency of gold nanoshells on silica nanorattles

Nanoscale ◽  
2012 ◽  
Vol 4 (11) ◽  
pp. 3523 ◽  
Author(s):  
Huiyu Liu ◽  
Tianlong Liu ◽  
Linlin Li ◽  
Nanjing Hao ◽  
Longfei Tan ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Conversion Efficiency ◽  
Gold Nanoshells ◽  
Size Dependent

scholarly journals Engineered ionizable lipid nanoparticles for targeted delivery of RNA therapeutics into different types of cells in the liver

2021 ◽  
Vol 7 (9) ◽  
pp. eabf4398
Author(s):  
M. Kim ◽  
M. Jeong ◽  
S. Hur ◽  
Y. Cho ◽  
J. Park ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Targeted Delivery ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Lipid Nanoparticles ◽  
Liver Sinusoidal Endothelial Cells ◽  
Main Challenge ◽  
Cell Type Specific ◽  
Selective Delivery

Ionizable lipid nanoparticles (LNPs) have been widely used for in vivo delivery of RNA therapeutics into the liver. However, a main challenge remains to develop LNP formulations for selective delivery of RNA into certain types of liver cells, such as hepatocytes and liver sinusoidal endothelial cells (LSECs). Here, we report the engineered LNPs for the targeted delivery of RNA into hepatocytes and LSECs. The effects of particle size and polyethylene glycol–lipid content in the LNPs were evaluated for the hepatocyte-specific delivery of mRNA by ApoE-mediated cellular uptake through low-density lipoprotein receptors. Targeted delivery of RNA to LSECs was further investigated using active ligands. Incorporation of mannose allowed the selective delivery of RNA to LSECs, while minimizing the unwanted cellular uptake by hepatocytes. These results demonstrate that engineered LNPs have great potential for the cell type–specific delivery of RNA into the liver and other tissues.

scholarly journals EFFECTS OF POLYMERIC NANOPARTICLE SURFACE PROPERTIES ON INTERACTION WITH BRAIN TUMOR ENVIRONMENT

Nano LIFE ◽  
2013 ◽  
Vol 03 (04) ◽  
pp. 1343003 ◽  
Author(s):  
BRANDON MATTIX ◽  
THOMAS MOORE ◽  
OLGA UVAROV ◽  
SAMUEL POLLARD ◽  
LAUREN O'DONNELL ◽  
...  
Keyword(s):  
Human Brain ◽  
Surface Charge ◽  
Cellular Uptake ◽  
Drug Clearance ◽  
Cellular Interaction ◽  
Normal Tissues ◽  
Poly Ethylene ◽  
Uptake Studies ◽  
Effect Of Surface

Current chemotherapy treatments are limited by poor drug solubility, rapid drug clearance and systemic side effects. Additionally, drug penetration into solid tumors is limited by physical diffusion barriers [e.g., extracellular matrix (ECM)]. Nanoparticle (NP) blood circulation half-life, biodistribution and ability to cross extracellular and cellular barriers will be dictated by NP composition, size, shape and surface functionality. Here, we investigated the effect of surface charge of poly(lactide)-poly(ethylene glycol) NPs on mediating cellular interaction. Polymeric NPs of equal sizes were used that had two different surface functionalities: negatively charged carboxyl ( COOH ) and neutral charged methoxy ( OCH 3). Cellular uptake studies showed significantly higher uptake in human brain cancer cells compared to noncancerous human brain cells, and negatively charged COOH NPs were uptaken more than neutral OCH 3 NPs in 2D culture. NPs were also able to load and control the release of paclitaxel (PTX) over 19 days. Toxicity studies in U-87 glioblastoma cells showed that PTX-loaded NPs were effective drug delivery vehicles. Effect of surface charge on NP interaction with the ECM was investigated using collagen in a 3D cellular uptake model, as collagen content varies with the type of cancer and the stage of the disease compared to normal tissues. Results demonstrated that NPs can effectively diffuse across an ECM barrier and into cells, but NP mobility is dictated by surface charge. In vivo biodistribution of OCH 3 NPs in intracranial tumor xenografts showed that NPs more easily accumulated in tumors with less collagen. These results indicate that a robust understanding of NP interaction with various tumor environments can lead to more effective patient-tailored therapies.

scholarly journals Spontaneous cellular uptake of exogenous messenger RNA in vivo is nucleic acid-specific, saturable and ion dependent

Gene Therapy ◽  
2007 ◽  
Vol 14 (15) ◽  
pp. 1175-1180 ◽  
Author(s):  
J Probst ◽  
B Weide ◽  
B Scheel ◽  
B J Pichler ◽  
I Hoerr ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Cellular Uptake ◽  
Messenger Rna

scholarly journals Cerium oxide nanoparticles at the nano-bio interface: size-dependent cellular uptake

2018 ◽  
Vol 46 (sup3) ◽  
pp. S956-S963 ◽  
Author(s):  
Sushant Singh ◽  
Anh Ly ◽  
Soumen Das ◽  
Tamil S. Sakthivel ◽  
Swetha Barkam ◽  
...  
Keyword(s):  
Cerium Oxide ◽  
Cellular Uptake ◽  
Cerium Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Size Dependent

scholarly journals Radiosynthesis of A Novel Antisense Imaging Probe Targeting LncRNA HOTAIR in Malignant Glioma.

2021 ◽  
Author(s):  
Jiongyu Ren ◽  
Xiyuan Zhang ◽  
Jiang Cao ◽  
Jiali Tian ◽  
Jin Luo ◽  
...  
Keyword(s):  
Malignant Glioma ◽  
Cellular Uptake ◽  
Gel Electrophoresis ◽  
Radiochemical Purity ◽  
Malignant Tumors ◽  
Biodistribution Study ◽  
Blocking Group ◽  
Uptake Test ◽  
Lncrna Hotair

Abstract Background: Long non-coding RNA (lncRNA) HOTAIR was manifested overexpressing and amplifying in many human carcinomas, which could serve as a useful target for cancer therapy. The 99mTc radiolabeled antisense oligonucleotides (ASON) could visualize the expression of HOTAIR and provide a diagnostic and therapeutic value for malignant tumors. The aim of this study was to radiosynthesis 99mTc with HOTAIR ASON and investigate the in vivo imaging in malignant glioma xenografts. Methods: The ASON targeting lncRNA HOTAIR as well as mismatched ASON probe (ASONM) were designed and modified. The radiolabeling of 99mTc with two probes was via the conjugation of bifunctional chelator HYNIC. Then the probes were purified by Sephadex G25 and tested for its radiolabeling efficiency and purity, as well as stability by iTLC and gel electrophoresis. Then the radiolabeled probes were transfected with lipofectamine 2000 for cellular uptake test and the next experimental use. Furthermore, biodistribution study and SPECT imaging was performed at different time after probes were intravenously injected in U87 tumor bearing nude mice models. All data were analyzed by statistical software. Results: The labeling efficiencies of 99mTc-HYNIC-ASON and 99mTc-HYNIC-ASONM measured by iTLC were (91 ±1.5) % and (90 ±0.6) %, respectively, and the radiochemical purity were more than 89%. The probes showed good stability within 12 hours for high radiochemical purity. Gel electrophoresis confirmed that the oligomers were successfully radiolabeled no significant degradation were found. Cellular uptake experiment showed that liposomes had ability to carry probes into cells. Biodistribution study demonstrated that liposome coated 99mTc-HYNIC-ASON had significantly higher uptake in the tumor and higher tumor to muscle ratio than mismatched group. Meanwhile tumor was clearly shown at 1 hours post probe injection of Liposome coated 99mTc-HYNIC-ASON on SPECT/CT imaging, compared with mismatched and blocking group. Conclusion: The Liposome encapsulated 99mTc-HYNIC-ASON probe can be radiosynthesized and used in the in vivo, real-time imaging of lncRNA HOTAIR expression in malignant glioma.

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