Activation of a silent phenazine biosynthetic gene cluster reveals a novel natural product and a new resistance mechanism against phenazines

MedChemComm ◽  
2012 ◽  
Vol 3 (8) ◽  
pp. 1009 ◽  
Author(s):  
Orwah Saleh ◽  
Tobias Bonitz ◽  
Katrin Flinspach ◽  
Andreas Kulik ◽  
Nadja Burkard ◽  
...  
Keyword(s):  
Natural Product ◽  
Gene Cluster ◽  
Resistance Mechanism ◽  
Biosynthetic Gene Cluster ◽  
Biosynthetic Gene

scholarly journals New Insights into the Genetic Organization of the FK228 Biosynthetic Gene Cluster inChromobacterium violaceumNo. 968

2010 ◽  
Vol 77 (4) ◽  
pp. 1508-1511 ◽  
Author(s):  
Vishwakanth Y. Potharla ◽  
Shane R. Wesener ◽  
Yi-Qiang Cheng
Keyword(s):  
Natural Product ◽  
Gene Cluster ◽  
Gene Deletion ◽  
Regulatory Gene ◽  
Biosynthetic Gene Cluster ◽  
Transcriptional Analysis ◽  
Biosynthetic Gene ◽  
Genetic Organization

ABSTRACTThe biosynthetic gene cluster of FK228, an FDA-approved anticancer natural product, was identified and sequenced previously. The genetic organization of this gene cluster has now been delineated through systematic gene deletion and transcriptional analysis. As a result, the gene cluster is redefined to contain 12 genes:depAthroughdepJ,depM, and a newly identified pathway regulatory gene,depR.

scholarly journals mSphere of Influence: Synthetic Biology of Natural Product Biosynthesis

mSphere ◽  
2020 ◽  
Vol 5 (1) ◽  
Author(s):  
Mark C. Walker
Keyword(s):  
Natural Products ◽  
Synthetic Biology ◽  
Natural Product ◽  
Gene Cluster ◽  
Biosynthetic Gene Cluster ◽  
Biosynthetic Gene ◽  
Natural Product Biosynthesis ◽  
Antibiotic Compounds ◽  
Approaches To Study

ABSTRACT Mark Walker studies the biosynthesis and engineering of bacterial natural products with the long-term goal of identifying new antibiotic compounds. In this mSphere of Influence, he reflects on how “Direct cloning and refactoring of a silent lipopeptide biosynthetic gene cluster yields the antibiotic taromycin A” by K. Yamanaka, K. A. Reynolds, R. D. Kersten, K. S. Ryan, et al. (Proc Natl Acad Sci USA 111:1957–1962, 2014, https://doi.org/10.1073/pnas.1319584111) impacted his thinking on using synthetic biology approaches to study natural product biosynthesis.

Cloning and Heterologous Expression of a Natural Product Biosynthetic Gene Cluster from eDNA

2001 ◽  
Vol 3 (13) ◽  
pp. 1981-1984 ◽  
Author(s):  
Sean F. Brady ◽  
Carol J. Chao ◽  
Jo Handelsman ◽  
Jon Clardy
Keyword(s):  
Heterologous Expression ◽  
Natural Product ◽  
Gene Cluster ◽  
Biosynthetic Gene Cluster ◽  
Biosynthetic Gene

scholarly journals Genome Mining and Metabolomics Uncover a Rare d-Capreomycidine Containing Natural Product and Its Biosynthetic Gene Cluster

2020 ◽  
Vol 15 (11) ◽  
pp. 3013-3020
Author(s):  
James H. Tryon ◽  
Jennifer C. Rote ◽  
Li Chen ◽  
Matthew T. Robey ◽  
Marvin M. Vega ◽  
...  
Keyword(s):  
Natural Product ◽  
Gene Cluster ◽  
Genome Mining ◽  
Biosynthetic Gene Cluster ◽  
Biosynthetic Gene
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