Single channel layer, single sheath-flow inlet microfluidic flow cytometer with three-dimensional hydrodynamic focusing

Lab on a Chip ◽  
2012 ◽  
Vol 12 (17) ◽  
pp. 3135 ◽  
Author(s):  
Shiang-Chi Lin ◽  
Pei-Wen Yen ◽  
Chien-Chung Peng ◽  
Yi-Chung Tung
Keyword(s):  
Single Channel ◽  
Three Dimensional ◽  
Flow Cytometer ◽  
Hydrodynamic Focusing ◽  
Sheath Flow ◽  
Channel Layer ◽  
Microfluidic Flow

Single-layer planar on-chip flow cytometer using microfluidic drifting based three-dimensional (3D) hydrodynamic focusing

Lab on a Chip ◽  
2009 ◽  
Vol 9 (11) ◽  
pp. 1583 ◽  
Author(s):  
Xiaole Mao ◽  
Sz-Chin Steven Lin ◽  
Cheng Dong ◽  
Tony Jun Huang
Keyword(s):  
Three Dimensional ◽  
Single Layer ◽  
Flow Cytometer ◽  
Hydrodynamic Focusing ◽  
Chip Flow ◽  
On Chip

Three-dimensional unsteady calculations for a flow cytometer

2008 ◽  
Vol 222 (4) ◽  
pp. 679-687
Author(s):  
A-S Yang ◽  
W-H Hsieh ◽  
L-Y Tseng
Keyword(s):  
Three Dimensional ◽  
Flow Cytometer ◽  
Hydrodynamic Focusing ◽  
Momentum Exchange ◽  
Two Fluids ◽  
Flow Transport ◽  
Fluid Properties ◽  
Transport Behaviour ◽  
Time Required ◽  
Flow Variables

The phenomenon of hydrodynamic focusing in a flow cytometer is investigated using a computational approach. In this work, a three-dimensional two-fluid theoretical model was established to describe the flow transport behaviour and the interaction of two fluids coflowing at different velocities. Treating both sample and sheath fluids as laminar, incompressible, and isothermal flows, the analysis encompasses two sets of three-dimensional unsteady equations for conservation of mass and momentum, with consideration of interfacial momentum exchange. Governing equations are solved numerically through an iterative semi-implicit method for pressure-linked equations consistent algorithm to determine the flow variables. For code validation, both focused width and length in the two-dimensional configuration are predicted at a broad range of ush/ us ratios and are compared with Lee et al.'s measured data. Subsequently, the work extends to examine the three-dimensional hydrodynamic focusing process and the time required for completion of one focusing event. To explore the feasibility of the proposed flow cytometer in applications, the focused properties are determined by varying the ratio of sheath velocity to sample velocity from 10 to 80. Ten numerical experiments were also conducted to examine the effects of the fluid properties on the length and width of the focused sample stream.

scholarly journals A 3D hydrodynamic flow-focusing device for cell sorting

2021 ◽  
Vol 25 (3) ◽  
Author(s):  
Xiaofei Yuan ◽  
Andrew Glidle ◽  
Hitoshi Furusho ◽  
Huabing Yin
Keyword(s):  
Cell Sorting ◽  
Control Strategies ◽  
Three Dimensional ◽  
Hydrodynamic Flow ◽  
Proof Of Concept ◽  
Flow Focusing ◽  
Hydrodynamic Focusing ◽  
Cell Handling ◽  
Fluid Flow Control ◽  
High Degree

AbstractOptical-based microfluidic cell sorting has become increasingly attractive for applications in life and environmental sciences due to its ability of sophisticated cell handling in flow. The majority of these microfluidic cell sorting devices employ two-dimensional fluid flow control strategies, which lack the ability to manipulate the position of cells arbitrarily for precise optical detection, therefore resulting in reduced sorting accuracy and purity. Although three-dimensional (3D) hydrodynamic devices have better flow-focusing characteristics, most lack the flexibility to arbitrarily position the sample flow in each direction. Thus, there have been very few studies using 3D hydrodynamic flow focusing for sorting. Herein, we designed a 3D hydrodynamic focusing sorting platform based on independent sheath flow-focusing and pressure-actuated switching. This design offers many advantages in terms of reliable acquisition of weak Raman signals due to the ability to precisely control the speed and position of samples in 3D. With a proof-of-concept demonstration, we show this 3D hydrodynamic focusing-based sorting device has the potential to reach a high degree of accuracy for Raman activated sorting.

A New Technology for Three-Dimensional Cell Culture: The Hydrodynamic Focusing Bioreactor

1999 ◽  
Author(s):  
Yow-Min D. Tsao ◽  
Steve R. Gonda
Keyword(s):  
Cell Culture ◽  
New Technology ◽  
Three Dimensional ◽  
Culture Vessel ◽  
Air Bubbles ◽  
Hydrodynamic Focusing ◽  
Shaped Cell ◽  
Fluid Environment ◽  
Dimensional Cell ◽  
Low Shear

Abstract The Hydrodynamic Focusing Bioreactor (HDFB) developed by NASA at the Johnson Space Center provides a unique hydrofocusing capability that simultaneously enables a low-shear culture environment and a unique hydrofocusing-based “herding” of suspended cells, cell aggregates, and air bubbles. The HDFB is a rotating dome-shaped cell culture vessel with a centrally located sampling port and an internal rotating viscous spinner attached to a rotating base. The vessel and viscous spinner can rotate at different speeds and in either the same or different directions. Adjusting the differential rotation rate between the vessel and spinner results in a controllable hydrodynamic focusing force. The resultant hydrodynamic force suspends the cells in a low-shear fluid environment that supports the formation of delicate three-dimensional tissue assemblies. Both suspension and anchorage-dependent cells have been successfully cultured.

scholarly journals 3D Hydrodynamic Focusing in Microscale Optofluidic Channels Formed with a Single Sacrificial Layer

Micromachines ◽  
2020 ◽  
Vol 11 (4) ◽  
pp. 349 ◽  
Author(s):  
Erik S. Hamilton ◽  
Vahid Ganjalizadeh ◽  
Joel G. Wright ◽  
Holger Schmidt ◽  
Aaron R. Hawkins
Keyword(s):  
Cross Sections ◽  
Optical Waveguides ◽  
Sacrificial Layer ◽  
Three Dimensional ◽  
Single Layer ◽  
Detection Sensitivity ◽  
Low Flow ◽  
Hydrodynamic Focusing ◽  
Induced Focusing ◽  
Pressure Driven Flow

Optofluidic devices are capable of detecting single molecules, but greater sensitivity and specificity is desired through hydrodynamic focusing (HDF). Three-dimensional (3D) hydrodynamic focusing was implemented in 10-μm scale microchannel cross-sections made with a single sacrificial layer. HDF is achieved using buffer fluid to sheath the sample fluid, requiring four fluid ports to operate by pressure driven flow. A low-pressure chamber, or pit, formed by etching into a substrate, enables volumetric flow ratio-induced focusing at a low flow velocity. The single layer design simplifies surface micromachining and improves device yield by 1.56 times over previous work. The focusing design was integrated with optical waveguides and used in order to analyze fluorescent signals from beads in fluid flow. The implementation of the focusing scheme was found to narrow the distribution of bead velocity and fluorescent signal, giving rise to 33% more consistent signal. Reservoir effects were observed at low operational vacuum pressures and a balance between optofluidic signal variance and intensity was achieved. The implementation of the design in optofluidic sensors will enable higher detection sensitivity and sample specificity.

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