Iterative operations on microdroplets and continuous monitoring of processes within them; determination of solubility diagrams of proteins

Lab on a Chip ◽  
2012 ◽  
Vol 12 (20) ◽  
pp. 4022 ◽  
Author(s):  
Monika E. Dolega ◽  
Slawomir Jakiela ◽  
Michal Razew ◽  
Agata Rakszewska ◽  
Olgierd Cybulski ◽  
...  
Keyword(s):  
Continuous Monitoring ◽  
Solubility Diagrams

scholarly journals Identification of the Trichinella species by PCR method

2012 ◽  
Vol 66 (1-2) ◽  
pp. 41-47
Author(s):  
Sasa Vasilev ◽  
Jelena Cvetkovic ◽  
Ivana Radovic ◽  
Ljiljana Sofronic-Milosavljevic
Keyword(s):  
Human Health ◽  
Health Problem ◽  
Continuous Monitoring ◽  
Human Consumption ◽  
Sylvatic Cycle ◽  
Trichinella Infection ◽  
High Prevalence ◽  
Pcr Method ◽  
Pig Meat

Serbia is country with a high prevalence of Trichinella infection in pigs, which continues to be a serious human health problem. In Serbia, only a few isolates of Trichinella found in pork have been genetically specified to date, and all were proven as T. spiralis. New data shows that in the sylvatic cycle in Serbia, at least in the Belgrade district, more than one Trichinella species co-exist (T. spiralis and T. britovi). Increased awareness of the possible overlap among sylvatic and domestic Trichinella cycles indicates the need for continuous monitoring of Trichinella species circulation and strongly points to the need that all isolates of Trichinella found in meat for human consumption should be subject to a determination of the Trichinella species (due to the risk of transmission of infection with T. britovi to domestic pigs and humans). This is why we examined using PCR the Trichinella larvae found in pig meat that caused a human outbreak (Trichinella infection) in Grocka (Belgrade district) during February 2011. The isolated larvae belonged to T. spiralis.

Near-IR Spectroscopic Determination of NaCl in Aqueous Solution

1992 ◽  
Vol 46 (12) ◽  
pp. 1809-1815 ◽  
Author(s):  
Jie Lin ◽  
Chris W. Brown
Keyword(s):  
Aqueous Solution ◽  
Temperature Effects ◽  
Continuous Monitoring ◽  
Principal Component Regression ◽  
Principal Component ◽  
Standard Errors ◽  
Near Ir ◽  
Linear And Nonlinear ◽  
Ir Spectroscopic

The concentrations of NaCl in aqueous solutions have been determined with the use of near-IR spectra between 1100 and 1900 nm. Models expressing the concentration of NaCl are developed with linear and nonlinear regression with the use of the absorbances at selected wavelengths and with principal component regression (PCR) using entire spectra. Temperature perturbations on water bands interfere with the measurement of NaCl but can be removed by linear or nonlinear regressions using the absorbances at the wavelengths where the temperature effects are zero, or they can be accounted for by PCR. Standard errors of 5 mM and a detection limit of IS mM are obtained for NaCl. This technique can be applied for quantitative analysis of NaCl in the laboratory or can be readily adapted for continuous monitoring in process control.

scholarly journals The Relative Merits and Applicability of Kinetic and Fixed-Incubation Methods of Enzyme Assay in Clinical Enzymology

1972 ◽  
Vol 18 (12) ◽  
pp. 1449-1454 ◽  
Author(s):  
D W Moss
Keyword(s):  
Enzyme Activity ◽  
Continuous Monitoring ◽  
Enzyme Assay ◽  
Kinetic Method ◽  
Reaction Progress ◽  
Kinetic Principle ◽  
Clinical Enzymology

Abstract The considerations are discussed that make continuous monitoring of reaction-progress curves superior to fixed-incubation methods in the determination of enzyme activity. Provided that they are used with caution and their limitations are appreciated, fixed-incubation methods continue to fulfill a useful, though diminishing, role in clinical enzymology because of their adaptability to existing patterns of automation. The introduction of suitable mechanized equipment will favor the eventual complete adoption of the kinetic method. However, the use of such equipment should not be at the expense of th important characteristics of the kinetic principle.

Determination of creatine kinase B subunit activity by continuous monitoring of ATP — a new bioluminescent technique applied to clinical chemistry

1979 ◽  
Vol 12 (6) ◽  
pp. 214-215 ◽  
Author(s):  
A. Lundin ◽  
W. Gerhardt ◽  
K. Lindberg ◽  
T. Lövgren ◽  
R. Nordlander ◽  
...  
Keyword(s):  
Creatine Kinase ◽  
Continuous Monitoring ◽  
Clinical Chemistry ◽  
B Subunit ◽  
Creatine Kinase B

scholarly journals Unsegmented flow approach for on-line monitoring of pH, conductivity, dissolved oxygen and determination of nitrite and ammonia in aquaculture

1994 ◽  
Vol 16 (2) ◽  
pp. 59-62 ◽  
Author(s):  
A. C. Ariza ◽  
P. Linares ◽  
M. D. Luque de Castro ◽  
M. Valcárcel
Keyword(s):  
Dissolved Oxygen ◽  
Continuous Monitoring ◽  
Sea Water ◽  
Flow System ◽  
Photometric Determination ◽  
Fish Feed ◽  
Fish Breeding ◽  
On Line ◽  
High Concentrations

A fully automated flow system for on-line monitoring of analytes/parameters of interest in aquaculture is described. The approach has been optimized for the photometric determination of nitrite and ammonia and the continuous monitoring of pH, conductivity and dissolved oxygen, but these analytes/parameters are readily changeable as required. The system has been tested by monitoring these species in the input and output sea water streams of tanks at a fish breeding farm and also by monitoring water containing high concentrations of fish feed.

Factors influencing left ventricular shortening in isolated canine heart

1976 ◽  
Vol 230 (2) ◽  
pp. 419-426 ◽  
Author(s):  
KT Weber ◽  
JS Janicki ◽  
RC Reeves ◽  
LL Hefner
Keyword(s):  
Continuous Monitoring ◽  
Ventricular Volume ◽  
Left Ventricular ◽  
Canine Heart ◽  
Cross Sectional ◽  
Contractile State ◽  
Positive Inotropic Agents ◽  
And Control ◽  
Initial Fiber

To assess in the intact ventricle the steady-state influence of several mechanical variables on the extent of left ventricular midwall circumferential shortening, a pressure servo system was utilized in isolated canine hearts. The system permits continuous monitoring of ventricular volume and control of diastolic and systolic pressures. After determination of the diastolic volume at zero filling pressure (V0), a series of variably preloaded or afterloaded contractions were generated including the isovolumic state. Contractile state was manipulated in a positive (calcium, 12-18 mg/100 ml; norepinephrine, 0.4-1.4 mug/min) or negative (propranolol, 0.12-0.50 mg/min) direction. Force and length terms derived for a thick-walled sphere were expressed per cross-sectional area of muscle and length, respectively, calculated at V0. For any preload, an inverse linear (r greater than 0.96) force-shortening relation was obtained, and each line was identified by its slope and isovolumic load (sigma0). Both slope and sigma0 increased with positive inotropic agents (vis-a-vis propranolol) or increments in preload. Thus, in the intact ventricle an inverse linear relation characterizes the force-shortening relation with the amount of shortening determined by initial fiber length, afterload, and the contractile state of the myocardium.

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