Rational design of metallophosphors with tunable aggregation-induced phosphorescent emission and their promising applications in time-resolved luminescence assay and targeted luminescence imaging of cancer cells

2012 ◽  
Vol 22 (41) ◽  
pp. 22167 ◽  
Author(s):  
Shujuan Liu ◽  
Huibin Sun ◽  
Yun Ma ◽  
Shanghui Ye ◽  
Xiangmei Liu ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Rational Design ◽  
Time Resolved ◽  
Luminescence Imaging ◽  
Luminescence Assay

scholarly journals Real-time observation of tetrapyrrole binding to an engineered bacterial phytochrome

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Yusaku Hontani ◽  
Mikhail Baloban ◽  
Francisco Velazquez Escobar ◽  
Swetta A. Jansen ◽  
Daria M. Shcherbakova ◽  
...  
Keyword(s):  
Real Time ◽  
Rational Design ◽  
Near Infrared ◽  
Fluorescent Proteins ◽  
Covalent Bond ◽  
Binding Pocket ◽  
Tissue Imaging ◽  
Covalent Attachment ◽  
Time Resolved

AbstractNear-infrared fluorescent proteins (NIR FPs) engineered from bacterial phytochromes are widely used for structural and functional deep-tissue imaging in vivo. To fluoresce, NIR FPs covalently bind a chromophore, such as biliverdin IXa tetrapyrrole. The efficiency of biliverdin binding directly affects the fluorescence properties, rendering understanding of its molecular mechanism of major importance. miRFP proteins constitute a family of bright monomeric NIR FPs that comprise a Per-ARNT-Sim (PAS) and cGMP-specific phosphodiesterases - Adenylyl cyclases - FhlA (GAF) domain. Here, we structurally analyze biliverdin binding to miRFPs in real time using time-resolved stimulated Raman spectroscopy and quantum mechanics/molecular mechanics (QM/MM) calculations. Biliverdin undergoes isomerization, localization to its binding pocket, and pyrrolenine nitrogen protonation in <1 min, followed by hydrogen bond rearrangement in ~2 min. The covalent attachment to a cysteine in the GAF domain was detected in 4.3 min and 19 min in miRFP670 and its C20A mutant, respectively. In miRFP670, a second C–S covalent bond formation to a cysteine in the PAS domain occurred in 14 min, providing a rigid tetrapyrrole structure with high brightness. Our findings provide insights for the rational design of NIR FPs and a novel method to assess cofactor binding to light-sensitive proteins.

scholarly journals Picosecond time-resolved photon antibunching measures nanoscale exciton motion and the true number of chromophores

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Gordon J. Hedley ◽  
Tim Schröder ◽  
Florian Steiner ◽  
Theresa Eder ◽  
Felix J. Hofmann ◽  
...  
Keyword(s):  
Rational Design ◽  
Three Dimensional ◽  
Dna Origami ◽  
Fluorescent Nanoparticles ◽  
Time Resolved ◽  
Exciton Diffusion ◽  
Polymer Aggregates ◽  
True Number ◽  
Particle Level ◽  
Photon Antibunching

AbstractThe particle-like nature of light becomes evident in the photon statistics of fluorescence from single quantum systems as photon antibunching. In multichromophoric systems, exciton diffusion and subsequent annihilation occurs. These processes also yield photon antibunching but cannot be interpreted reliably. Here we develop picosecond time-resolved antibunching to identify and decode such processes. We use this method to measure the true number of chromophores on well-defined multichromophoric DNA-origami structures, and precisely determine the distance-dependent rates of annihilation between excitons. Further, this allows us to measure exciton diffusion in mesoscopic H- and J-type conjugated-polymer aggregates. We distinguish between one-dimensional intra-chain and three-dimensional inter-chain exciton diffusion at different times after excitation and determine the disorder-dependent diffusion lengths. Our method provides a powerful lens through which excitons can be studied at the single-particle level, enabling the rational design of improved excitonic probes such as ultra-bright fluorescent nanoparticles and materials for optoelectronic devices.

Ratiometric Iridium(III) Complex-Based Phosphorescent Chemodosimeter for Hg2+ Applicable in Time-Resolved Luminescence Assay and Live Cell Imaging

2015 ◽  
Vol 87 (6) ◽  
pp. 3255-3262 ◽  
Author(s):  
Jiaxi Ru ◽  
Xu Chen ◽  
Liping Guan ◽  
Xiaoliang Tang ◽  
Chunming Wang ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Time Resolved ◽  
Luminescence Assay

An integrin-targeting nanosystem as a carrier of the selenadiazole derivative to induce ROS-mediated apoptosis in bladder cancer cells, from rational design to action mechanisms

2015 ◽  
Vol 3 (48) ◽  
pp. 9374-9382 ◽  
Author(s):  
Yifan Wang ◽  
Wenying Li ◽  
Yahui Yang ◽  
Qinsong Zeng ◽  
Ka-Hing Wong ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Cancer Cells ◽  
Rational Design ◽  
Action Mechanisms ◽  
Bladder Cancer Cells

Herein an integrin-targeting nanosystem is rationally designed and used as a carrier of a selenadiazole derivative to induce ROS-mediated apoptosis in bladder cancer cells.

scholarly journals Targeting cancer cell metabolism with mitochondria-immobilized phosphorescent cyclometalated iridium(iii) complexes

2017 ◽  
Vol 8 (1) ◽  
pp. 631-640 ◽  
Author(s):  
Jian-Jun Cao ◽  
Cai-Ping Tan ◽  
Mu-He Chen ◽  
Na Wu ◽  
De-Yang Yao ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Cancer Cell ◽  
Rational Design ◽  
Cell Metabolism ◽  
Mitochondrial Metabolism ◽  
Cancer Cell Metabolism

We report a rational design and mechanism studies of mitochondria-immobilized iridium(iii) complexes that can kill cancer cells by targeting mitochondrial metabolism.

Rational design of water-dispersible and biocompatible nanoprobes with H2S-triggered NIR emission for cancer cell imaging

2020 ◽  
Vol 8 (28) ◽  
pp. 6013-6016
Author(s):  
Hengyan Liu ◽  
Ge Xu ◽  
Tianli Zhu ◽  
Rongchen Wang ◽  
Jiahui Tan ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Cancer Cell ◽  
Small Molecule ◽  
Rational Design ◽  
Cell Imaging ◽  
Imaging Modality ◽  
Aqueous Solubility ◽  
Water Dispersible ◽  
Nir Emission ◽  
Small Molecule Probe

A nanoprobe with good aqueous solubility and biocompatibility by trapping an H2S-activatable small molecule probe in the interior of surface cross-linked micelles was fabricated for imaging of H2S-rich cancer cells in a dual-color imaging modality.

Solvent exchange in a metal–organic framework single crystal monitored by dynamicin situX-ray diffraction

2017 ◽  
Vol 73 (4) ◽  
pp. 669-674 ◽  
Author(s):  
Jordan M. Cox ◽  
Ian M. Walton ◽  
Gage Bateman ◽  
Cassidy A. Benson ◽  
Travis Mitchell ◽  
...  
Keyword(s):  
Rational Design ◽  
Metal Organic Framework ◽  
X Ray Diffraction ◽  
Guest Molecules ◽  
Time Resolved ◽  
Ethanol Solvate ◽  
Significant Step ◽  
Metal Organic ◽  
Flexible Metal ◽  
Organic Framework

Understanding the processes by which porous solid-state materials adsorb and release guest molecules would represent a significant step towards developing rational design principles for functional porous materials. To elucidate the process of liquid exchange in these materials, dynamicin situX-ray diffraction techniques have been developed which utilize liquid-phase chemical stimuli. Using these time-resolved diffraction techniques, the ethanol solvation process in a flexible metal–organic framework [Co(AIP)(bpy)0.5(H2O)]·2H2O was examined. The measurements provide important insight into the nature of the chemical transformation in this system including the presence of a previously unreported neat ethanol solvate structure.

scholarly journals Probing biotin receptors in cancer cells with rationally designed fluorogenic squaraine dimers

2020 ◽  
Vol 11 (31) ◽  
pp. 8240-8248 ◽  
Author(s):  
Kyong T. Fam ◽  
Mayeul Collot ◽  
Andrey S. Klymchenko
Keyword(s):  
Cancer Cells ◽  
Rational Design ◽  
Fluorogenic Probe ◽  
Cancerous Cells

Rational design of self-quenched squaraine dimers bearing biotin yielded a bright fluorogenic probe that can distinguish cancerous from non-cancerous cells.

scholarly journals A rational design of a cancer-specific and lysosome-targeted fluorescence nanoprobe for glutathione imaging in living cells

2020 ◽  
Vol 1 (6) ◽  
pp. 1739-1744
Author(s):  
Hong Wang ◽  
Peisheng Zhang ◽  
Chonghua Zhang ◽  
Shu Chen ◽  
Rongjin Zeng ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Cancer Cell ◽  
Rational Design ◽  
Living Cells ◽  
Dual Targeting ◽  
Fluorescence Nanoprobe

A dual-targeting (both cancer cell- and lysosome-targeting) fluorescence nanoprobe was rational designed and synthesized for the efficient imaging of lysosomal GSH in cancer cells.

Sign in / Sign up

Export Citation Format

Share Document