Tuning the surface chemistry of iPDMS for improved protein microarray performance

2012 ◽  
Vol 22 (13) ◽  
pp. 6327 ◽  
Author(s):  
Xing Liu ◽  
Yuanzi Wu ◽  
Ya Gao ◽  
Jie Wang ◽  
Zhong Li ◽  
...  
Keyword(s):  
Surface Chemistry ◽  
Protein Microarray

Protein microarray spots are modulated by patterning method, surface chemistry and processing conditions

2019 ◽  
Vol 130 ◽  
pp. 397-407 ◽  
Author(s):  
Kathryn F.A. Clancy ◽  
Sebastien Dery ◽  
Veronique Laforte ◽  
Prasad Shetty ◽  
David Juncker ◽  
...  
Keyword(s):  
Surface Chemistry ◽  
Protein Microarray ◽  
Processing Conditions

SURFACE CHEMISTRY FOR PROTEIN MICROARRAYS

2007 ◽  
Vol 06 (02) ◽  
pp. 109-116 ◽  
Author(s):  
ATHENA GUO ◽  
XIAOYANG ZHU
Keyword(s):  
Surface Chemistry ◽  
Protein Microarray ◽  
Three Dimensional ◽  
Protein Molecule ◽  
Protein Microarrays ◽  
Protein Chip ◽  
Dimensional Structure ◽  
Ideal Situation ◽  
Protein Molecules ◽  
Microarray Fabrication

Protein microarray or protein chip is an important tool in proteomics. However, duplicating the success of the DNA chip for the protein chip has been difficult. This account discusses a key issue in protein microarray development, i.e., surface chemistry. Ideally, the surface chemistry for protein microarray fabrication should satisfy the following criteria: the surface resists nonspecific adsorption; functional groups for the facile immobilization of protein molecules of interest are readily available; bonding between a protein molecule and a solid surface is balanced to provide sufficient stability but minimal disturbance on the delicate three-dimensional structure of the protein; linking chemistry allows the control of protein orientation; the local chemical environment favors the immobilized protein molecules to retain their native conformation; and finally, the specificity of linking chemistry is so high that no pre-purification of proteins is required. Strategies to achieve such an ideal situation are discussed, with successful examples from our laboratories illustrated. Finally, the need of surface technology for membrane protein microarray fabrication is addressed.

Photoluminescence behavior of silicon nanocrystals: Role of surface chemistry and size

2008 ◽  
Author(s):  
Anoop Gupta ◽  
Folarin Erogbogbo ◽  
Mark T. Swihart ◽  
Hartmut Wiggers
Keyword(s):  
Surface Chemistry ◽  
Silicon Nanocrystals ◽  
Photoluminescence Behavior

Improved Autoadhesion Measurement Method for Micromachined Polysilicon Beams

1996 ◽  
Vol 444 ◽  
Author(s):  
Maarten P. de Boer ◽  
Terry A. Michalske
Keyword(s):  
Surface Chemistry ◽  
Optical Microscopy ◽  
Measurement Method ◽  
Current Practice ◽  
Cantilever Beams ◽  
Beam Length ◽  
Substrate Adhesion

AbstractWe have measured autoadhesion (e.g. stiction) of individual polysilicon beams by interferometric optical microscopy. Untreated cantilever beams were dried from water in air, while treated beams were coated with a hydrophobic molecular coating of octadecyltrichlorosilane (ODTS). Adhesion values obtained for beams adhered to the substrate over a long length (large d) are independent of beam length with values of 16.7 and 4.4 mJ/m2 for untreated and treated samples respectively. These values can be understood in terms of differences in surface chemistry and polysilicon roughness. Using the shortest length beam which remains attached to the substrate, adhesion values were 280 and 16 mJ/m2 respectively. These higher values may be a result of capillarity effects. We recommend that measurements be made on beams in which d is large, in contrast to the current practice of noting the shortest beam adhered.

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