Elemental mass spectrometry for Se-dependent glutathione peroxidase determination in red blood cells as oxidative stress biomarker

2012 ◽  
Vol 27 (11) ◽  
pp. 1949 ◽  
Author(s):  
Juan Gómez-Espina ◽  
Elisa Blanco-González ◽  
Maria Montes-Bayón ◽  
Alfredo Sanz-Medel
Keyword(s):  
Oxidative Stress ◽  
Mass Spectrometry ◽  
Glutathione Peroxidase ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Elemental Mass ◽  
Elemental Mass Spectrometry ◽  
Stress Biomarker ◽  
Oxidative Stress Biomarker

scholarly journals Evaluation of Polyphenol Content and Antioxidant Capacity of Aqueous Extracts from Eight Medicinal Plants from Reunion Island: Protection against Oxidative Stress in Red Blood Cells and Preadipocytes

Antioxidants ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 959
Author(s):  
Eloïse Checkouri ◽  
Franck Reignier ◽  
Christine Robert-Da Silva ◽  
Olivier Meilhac
Keyword(s):  
Oxidative Stress ◽  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Medicinal Plants ◽  
Antioxidant Capacity ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Aqueous Extracts ◽  
Polyphenol Content ◽  
Réunion Island

Background—Medicinal plants are traditionally used as infusions or decoctions for their antioxidant, anti-inflammatory, hypolipidemic and anti-diabetic properties. Purpose—The aim of the study was to define the polyphenol composition and to assess the antioxidant capacity of eight medicinal plants from Reunion Island referred to in the French Pharmacopeia, namely Aphloia theiformis, Ayapana triplinervis, Dodonaea viscosa, Hubertia ambavilla, Hypericum lanceolatum, Pelargonium x graveolens, Psiloxylon mauritianum and Syzygium cumini. Methods—Polyphenol content was assessed by biochemical assay and liquid chromatography coupled to mass spectrometry. Antioxidant capacity was assessed by measuring DPPH reduction and studying the protective effects of herbal preparation on red blood cells or preadipocytes exposed to oxidative stress. Results—Polyphenol content ranged from 25 to 143 mg gallic acid equivalent (GAE)/L for infusions and 35 to 205 mg GAE/L for decoctions. Liquid chromatography coupled to mass spectrometry analysis showed the presence of major bioactive polyphenols, such as quercetin, chlorogenic acid, procyanidin and mangiferin. Antioxidant capacity assessed by different tests, including DPPH and Human red blood cell (RBC) hemolysis of herbal preparations, demonstrated a dose-dependent effect whatever the extraction procedure. Our data suggest that decoction slightly improved polyphenol extraction as well as antioxidant capacity relative to the infusion mode of extraction (DPPH test). However, infusions displayed a better protective effect against oxidative stress-induced RBC hemolysis. Conclusion—Traditional preparations of medicinal plant aqueous extracts (infusions and decoctions) display antioxidant properties that limit oxidative stress in preadipocytes and red blood cells, supporting their use in the context of metabolic disease prevention and treatment.

Effects of Nickel on Human and Fish Red Blood Cells

2007 ◽  
Vol 27 (4-5) ◽  
pp. 265-273 ◽  
Author(s):  
Grazia De Luca ◽  
Tiziana Gugliotta ◽  
Giulia Parisi ◽  
Pietro Romano ◽  
Antonella Geraci ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Rainbow Trout ◽  
Glutathione Peroxidase ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Nickel Chloride ◽  
Nickel Concentration ◽  
High Concentration ◽  
Consequent Increase

The objective of this study was to assess the effects of nickel chloride on human and rainbow trout erythrocytes in vitro. The cells were incubated with 0, 0.5 and 1 mM nickel chloride for 1 h at pH 7.40 and 25°C, then K+ efflux, SO42− uptake and GSH and GSSG concentrations were measured. In both kind of cells, “high concentration” nickel treatment increased KCl efflux with respect to the control. The SO42− uptake was not significantly different at “low nickel concentration” but was lower in erythrocytes treated with 1 mM nickel chloride; the rate constant of SO42− uptake decreased by 35% in human erythrocytes and by 44% in fish erythrocytes. Nickel chloride also acts on cellular metabolism and in particular on erythrocyte glutathione peroxidase with consequent increase in oxidative stress; the data show a significant decrease in intracellular GSH in both human (25%) and fish erythrocytes (18%) after treatment with nickel chloride, with concomitantly high GSSG concentrations and lower GSH/GSSG ratios.

Monitoring of Urinary Oxidative Stress Biomarker Levels in Newborns with Hypoxic-Ischemic Encephalopathy

2020 ◽  
Vol 159 ◽  
pp. S115
Author(s):  
Mari Merce Cascant Vilaplana ◽  
Ángel Sánchez-Illana ◽  
José David Piñeiro-Ramos ◽  
Guillermo Quintás ◽  
Camille Oger ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hypoxic Ischemic Encephalopathy ◽  
Ischemic Encephalopathy ◽  
Stress Biomarker ◽  
Oxidative Stress Biomarker
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