Water quality assessment using the AREc32 reporter gene assay indicative of the oxidative stress response pathway

2012 ◽  
Vol 14 (11) ◽  
pp. 2877 ◽  
Author(s):  
Beate I. Escher ◽  
Mriga Dutt ◽  
Erin Maylin ◽  
Janet Y. M. Tang ◽  
Simon Toze ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Water Quality ◽  
Stress Response ◽  
Quality Assessment ◽  
Reporter Gene ◽  
Water Quality Assessment ◽  
Oxidative Stress Response ◽  
Reporter Gene Assay ◽  
Stress Response Pathway ◽  
Gene Assay

scholarly journals In vitro bioanalytical assessment of toxicity of wetland samples from Spanish Mediterranean coastline

2021 ◽  
Vol 33 (1) ◽  
Author(s):  
Alberto Celma ◽  
Geeta Mandava ◽  
Agneta Oskarsson ◽  
Juan Vicente Sancho ◽  
Lubertus Bijlsma ◽  
...  
Keyword(s):  
Water Quality ◽  
Reporter Gene ◽  
Biological Activities ◽  
Reporter Gene Assay ◽  
Water Bodies ◽  
Good Tool ◽  
Adverse Health Effects ◽  
Aryl Hydrocarbon ◽  
Gene Assay

Abstract Background Fresh water bodies represent less than 1% of overall amount of water on earth and ensuring their quality and sustainability is pivotal. Although several campaigns have been performed to monitor the occurrence of micropollutants by means of chemical analysis, this might not cover the whole set of chemicals present in the sample nor the potential toxic effects of mixtures of natural and anthropogenic chemicals. In this sense, by selecting relevant toxicity endpoints when performing in vitro bioanalysis, effect-based methodologies can be of help to perform a comprehensive assessment of water quality and reveal biological activities relevant to adverse health effects. However, no prior bioanalytical study was performed in wetland water samples from the Spanish Mediterranean coastline. Methods Eleven samples from relevant water bodies from the Spanish Mediterranean coastline were collected to monitor water quality on 8 toxicity endpoints. Aryl hydrocarbon receptor (AhR), androgenicity (AR+ and AR−), estrogenicity (ER+ and ER−), oxidative stress response (Nrf2) and vitamin D receptor (VDR+ and VDR−) reporter gene assays were evaluated. Results AhR was the reporter gene assay showing a more frequent response over the set of samples (activated by 9 out of 11 samples), with TCDD-eq in the range 7.7–22.2 pM. For AR, ER and VDR assays sporadic activations were observed. Moreover, no activity was observed on the Nrf2 reporter gene assay. Wastewater and street runaway streams from Valencia could be responsible for enhanced activities in one of the water inputs in the Natural Park ‘L’Albufera’. Conclusions Water quality of relevant wetlands from the Spanish Mediterranean coastline has been evaluated. The utilization of a panel of 5 different bioassays to cover for different toxicity endpoints has demonstrated to be a good tool to assess water quality.

scholarly journals Synergistic Activation of the Nrf2-ARE Oxidative Stress Response Pathway by a Combination of Botanical Extracts

2016 ◽  
Vol 3 (02) ◽  
pp. e27-e30 ◽  
Author(s):  
Stephen Missler ◽  
Arun Rajgopal ◽  
Samantha Roloff ◽  
Jeffrey Scholten ◽  
Charlie Burns ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Stress Response ◽  
Oxidative Stress Response ◽  
Synergistic Activation ◽  
Stress Response Pathway ◽  
Botanical Extracts

scholarly journals The Translation Elongation Factor eEF1B Plays a Role in the Oxidative Stress Response Pathway

RNA Biology ◽  
2004 ◽  
Vol 1 (2) ◽  
pp. 89-94 ◽  
Author(s):  
Olubunmi Olarewaju ◽  
Pedro A. Ortiz ◽  
Wasimul Q. Chowdhury ◽  
Ishita Chatterjee ◽  
Terri Goss Kinzy
Keyword(s):  
Oxidative Stress ◽  
Stress Response ◽  
Elongation Factor ◽  
Oxidative Stress Response ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Stress Response Pathway

scholarly journals Comparative oxidative stress elicited by nanosilver in stable HSPA1A promoter-driven luciferase reporter HepG2 and A549 cells

2016 ◽  
Vol 5 (5) ◽  
pp. 1298-1305 ◽  
Author(s):  
Lili Xin ◽  
Jianshu Wang ◽  
Guoqiang Fan ◽  
Bizhong Che ◽  
Kaiming Cheng ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Reporter Gene ◽  
A549 Cells ◽  
Reporter Gene Assay ◽  
Luciferase Reporter ◽  
Luciferase Reporter Gene ◽  
Luciferase Reporter Gene Assay ◽  
Gene Assay

HSPA1A promoter-driven luciferase reporter gene assay provides a novel tool for predictive screening of the oxidative stress elicited by nanosilver.

Most Oxidative Stress Response In Water Samples Comes From Unknown Chemicals: The Need For Effect-Based Water Quality Trigger Values

2013 ◽  
Vol 47 (13) ◽  
pp. 7002-7011 ◽  
Author(s):  
Beate I. Escher ◽  
Charlotte van Daele ◽  
Mriga Dutt ◽  
Janet Y. M. Tang ◽  
Rolf Altenburger
Keyword(s):  
Oxidative Stress ◽  
Water Quality ◽  
Stress Response ◽  
Water Samples ◽  
Oxidative Stress Response

Unbiased compound screening with a reporter gene assay highlights the role of p13 in the cardiac cellular stress response

2018 ◽  
Vol 495 (2) ◽  
pp. 1992-1997
Author(s):  
Naoki Inoue ◽  
Taisei Hirouchi ◽  
Atsushi Kasai ◽  
Shintaro Higashi ◽  
Natsumi Hiraki ◽  
...  
Keyword(s):  
Stress Response ◽  
Reporter Gene ◽  
Cellular Stress ◽  
Reporter Gene Assay ◽  
Cellular Stress Response ◽  
Gene Assay ◽  
Compound Screening

scholarly journals A Combination of Genomic Approaches Reveals the Role of FOXO1a in Regulating an Oxidative Stress Response Pathway

PLoS ONE ◽  
2008 ◽  
Vol 3 (2) ◽  
pp. e1670 ◽  
Author(s):  
Paola de Candia ◽  
Ran Blekhman ◽  
Adrien E. Chabot ◽  
Alicia Oshlack ◽  
Yoav Gilad
Keyword(s):  
Oxidative Stress ◽  
Stress Response ◽  
Oxidative Stress Response ◽  
Stress Response Pathway
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