In vivo monitoring of hydrogen sulfide using a cresyl violet-based ratiometric fluorescence probe

2013 ◽  
Vol 49 (5) ◽  
pp. 502-504 ◽  
Author(s):  
Qiongqiong Wan ◽  
Yanchao Song ◽  
Zhao Li ◽  
Xinghui Gao ◽  
Huimin Ma
Keyword(s):  
Hydrogen Sulfide ◽  
Fluorescence Probe ◽  
Cresyl Violet ◽  
Ratiometric Fluorescence ◽  
In Vivo Monitoring

Imaging of living cells and zebrafish in vivo using a ratiometric fluorescent probe for hydrogen sulfide

The Analyst ◽  
2015 ◽  
Vol 140 (21) ◽  
pp. 7165-7169 ◽  
Author(s):  
Tianbao Liu ◽  
Jie Lin ◽  
Zhen Li ◽  
Lin Lin ◽  
Yuning Shen ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Fluorescent Probe ◽  
Fluorescence Probe ◽  
Living Cells ◽  
Ratiometric Fluorescence ◽  
Ratiometric Fluorescent Probe

We have developed a novel colorimetric and ratiometric fluorescence probe for the selective and sensitive imaging of H2S in living cells and living zebrafish in vivo.

Naked-eye and ratiometric fluorescence probe for fast and sensitive detection of hydrogen sulfide and its application in bioimaging

2018 ◽  
Vol 42 (23) ◽  
pp. 19272-19278 ◽  
Author(s):  
Jianlong Ma ◽  
Feifei Li ◽  
Qiang Li ◽  
Yijing Li ◽  
Chaoxian Yan ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Hela Cells ◽  
Fluorescence Probe ◽  
Sensitive Detection ◽  
Ratiometric Fluorescence ◽  
Naked Eye

A sensitive and ultrafast ratiometric fluorescence probe for the detection of hydrogen sulfide (H2S) in HeLa cells and zebrafish.

A ratiometric fluorescence probe for detection of hydrogen sulfide in cells

RSC Advances ◽  
2016 ◽  
Vol 6 (43) ◽  
pp. 36906-36909 ◽  
Author(s):  
Bin Wang ◽  
Na Jiang ◽  
Wei Sun ◽  
Qiufen Wang ◽  
Gengxiu Zheng
Keyword(s):  
Hydrogen Sulfide ◽  
Near Infrared ◽  
High Selectivity ◽  
Fluorescence Probe ◽  
Living Cells ◽  
Ratiometric Fluorescence ◽  
In Cells

A near-infrared ratiometric fluorescence probe, BOC, was developed for detection of H2S. The probe features high selectivity and sensitivity. And BOC has the ability to detect H2S in living cells.

scholarly journals A ratiometric fluorescence probe for the selective detection of H2S in serum using a pyrene-DPA–Cd2+ complex

RSC Advances ◽  
2021 ◽  
Vol 11 (39) ◽  
pp. 24410-24415
Author(s):  
Jihoon Kim ◽  
Jinyoung Oh ◽  
Min Su Han
Keyword(s):  
Hydrogen Sulfide ◽  
Fluorescence Probe ◽  
Selective Detection ◽  
Ratiometric Fluorescence ◽  
Displacement Approach

The pyrene-DPA–Cd2+ complex is demonstrated as a ratiometric fluorescence probe for selective hydrogen sulfide detection in serum based on a metal displacement approach.

A cyanine-based colorimetric and fluorescence probe for detection of hydrogen sulfide in vivo

2017 ◽  
Vol 247 ◽  
pp. 883-888 ◽  
Author(s):  
Huan Wang ◽  
Dan Yang ◽  
Rui Tan ◽  
Zhang Jian Zhou ◽  
Rui Xu ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Fluorescence Probe

An ICT-based strategy to a colorimetric and ratiometric fluorescence probe for hydrogen sulfide in living cells

2012 ◽  
Vol 48 (23) ◽  
pp. 2852 ◽  
Author(s):  
Fabiao Yu ◽  
Peng Li ◽  
Ping Song ◽  
Bingshuai Wang ◽  
Jianzhang Zhao ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Fluorescence Probe ◽  
Living Cells ◽  
Ratiometric Fluorescence

A novel two-photon fluorescent probe for hydrogen sulfide in living cells using an acedan–NBD amine dyad based on FRET process with high selectivity and sensitivity

2017 ◽  
Vol 41 (14) ◽  
pp. 6769-6774 ◽  
Author(s):  
Yao Tang ◽  
Guo-Fang Jiang
Keyword(s):  
Hydrogen Sulfide ◽  
Linear Response ◽  
High Selectivity ◽  
Fluorescence Probe ◽  
Living Cells ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

The first NBD amine based two-photon fluorescence probe L using a FRET strategy was developed for the H2S detecting in vitro and in vivo. The probe L not only afforded high selectivity and sensitivity for H2S detecting, but also dispalyed a linear response to H2S with a low detection limit 24 nM.

Endogenous hydrogen sulfide sulfhydrates IKKβ at cysteine 179 to control pulmonary artery endothelial cell inflammation

2019 ◽  
Vol 133 (20) ◽  
pp. 2045-2059 ◽  
Author(s):  
Da Zhang ◽  
Xiuli Wang ◽  
Siyao Chen ◽  
Selena Chen ◽  
Wen Yu ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Endothelial Cell ◽  
Pulmonary Artery ◽  
Cell Model ◽  
Site Directed Mutagenesis ◽  
Critical Event ◽  
Hypertensive Rats ◽  
Pulmonary Artery Endothelial Cell

Abstract Background: Pulmonary artery endothelial cell (PAEC) inflammation is a critical event in the development of pulmonary arterial hypertension (PAH). However, the pathogenesis of PAEC inflammation remains unclear. Methods: Purified recombinant human inhibitor of κB kinase subunit β (IKKβ) protein, human PAECs and monocrotaline-induced pulmonary hypertensive rats were employed in the study. Site-directed mutagenesis, gene knockdown or overexpression were conducted to manipulate the expression or activity of a target protein. Results: We showed that hydrogen sulfide (H2S) inhibited IKKβ activation in the cell model of human PAEC inflammation induced by monocrotaline pyrrole-stimulation or knockdown of cystathionine γ-lyase (CSE), an H2S generating enzyme. Mechanistically, H2S was proved to inhibit IKKβ activity directly via sulfhydrating IKKβ at cysteinyl residue 179 (C179) in purified recombinant IKKβ protein in vitro, whereas thiol reductant dithiothreitol (DTT) reversed H2S-induced IKKβ inactivation. Furthermore, to demonstrate the significance of IKKβ sulfhydration by H2S in the development of PAEC inflammation, we mutated C179 to serine (C179S) in IKKβ. In purified IKKβ protein, C179S mutation of IKKβ abolished H2S-induced IKKβ sulfhydration and the subsequent IKKβ inactivation. In human PAECs, C179S mutation of IKKβ blocked H2S-inhibited IKKβ activation and PAEC inflammatory response. In pulmonary hypertensive rats, C179S mutation of IKKβ abolished the inhibitory effect of H2S on IKKβ activation and pulmonary vascular inflammation and remodeling. Conclusion: Collectively, our in vivo and in vitro findings demonstrated, for the first time, that endogenous H2S directly inactivated IKKβ via sulfhydrating IKKβ at Cys179 to inhibit nuclear factor-κB (NF-κB) pathway activation and thereby control PAEC inflammation in PAH.

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