Simple and sensitive fluorescence detection of the RNA endonuclease activity of mammalian argonaute2 protein based on an RNA molecular beacon

2012 ◽  
Vol 48 (100) ◽  
pp. 12192 ◽  
Author(s):  
Feng Li ◽  
Peng Li ◽  
Limin Yang ◽  
Bo Tang
Keyword(s):  
Fluorescence Detection ◽  
Molecular Beacon ◽  
Endonuclease Activity ◽  
Rna Endonuclease

Tricolour fluorescence detection of sequence-specific DNA with a new molecular beacon and a nucleic acid dye TOTO-3

The Analyst ◽  
2012 ◽  
Vol 137 (24) ◽  
pp. 5898 ◽  
Author(s):  
Dongshan Xiang ◽  
Cuiling Zhang ◽  
Lu Chen ◽  
Xinghu Ji ◽  
Zhike He
Keyword(s):  
Nucleic Acid ◽  
Fluorescence Detection ◽  
Molecular Beacon ◽  
Acid Dye

scholarly journals PPR-SMR protein SOT1 has RNA endonuclease activity

2017 ◽  
Vol 114 (8) ◽  
pp. E1554-E1563 ◽  
Author(s):  
Wen Zhou ◽  
Qingtao Lu ◽  
Qingwei Li ◽  
Lei Wang ◽  
Shunhua Ding ◽  
...  
Keyword(s):  
Endonuclease Activity ◽  
Pentatricopeptide Repeat ◽  
Rna Sequence ◽  
Ppr Protein ◽  
Specific Manner ◽  
Rna Endonuclease

Numerous attempts have been made to identify and engineer sequence-specific RNA endonucleases, as these would allow for efficient RNA manipulation. However, no natural RNA endonuclease that recognizes RNA in a sequence-specific manner has been described to date. Here, we report that SUPPRESSOR OF THYLAKOID FORMATION 1 (SOT1), anArabidopsispentatricopeptide repeat (PPR) protein with a small MutS-related (SMR) domain, has RNA endonuclease activity. We show that the SMR moiety of SOT1 performs the endonucleolytic maturation of 23S and 4.5S rRNA through the PPR domain, specifically recognizing a 13-nucleotide RNA sequence in the 5′ end of the chloroplast 23S–4.5S rRNA precursor. In addition, we successfully engineered the SOT1 protein with altered PPR motifs to recognize and cleave a predicted RNA substrate. Our findings point to SOT1 as an exciting tool for RNA manipulation.

Fluorescence detection of adenosine triphosphate through an aptamer–molecular beacon multiple probe

2012 ◽  
Vol 424 (1) ◽  
pp. 8-11 ◽  
Author(s):  
Xiaodan Zeng ◽  
Xiaoling Zhang ◽  
Wen Yang ◽  
Hongying Jia ◽  
Yamin Li
Keyword(s):  
Adenosine Triphosphate ◽  
Fluorescence Detection ◽  
Molecular Beacon ◽  
Multiple Probe

Visual fluorescence detection of H2O2 and glucose based on “molecular beacon”-hosted Hoechst dyes

The Analyst ◽  
2015 ◽  
Vol 140 (10) ◽  
pp. 3642-3647 ◽  
Author(s):  
Ling-Fei Lu ◽  
Yan-Yun Li ◽  
Min Zhang ◽  
Guoyue Shi
Keyword(s):  
Fluorescence Detection ◽  
Molecular Beacon ◽  
Label Free ◽  
Single Stranded Dna

In this work, a label-free molecular beacon (MB)-like biosensor is designed for the determination of H2O2 and glucose based on the fluorescence regulation of Hoechst dyes hosted by the designed AT-rich single-stranded DNA (ssDNA), in which Hg2+ and cysteine (Cys) act as activators.

Tools for Investigation of the RNA Endonuclease Activity of Mammalian Argonaute2 Protein

2012 ◽  
Vol 84 (5) ◽  
pp. 2492-2497 ◽  
Author(s):  
Nana Yang ◽  
Ya Cao ◽  
Ping Han ◽  
Xuejun Zhu ◽  
Lizhou Sun ◽  
...  
Keyword(s):  
Endonuclease Activity ◽  
Rna Endonuclease

A novel graphene oxide-based fluorescence assay for RNA endonuclease activity of mammalian Argonaute2 protein

2013 ◽  
Vol 182 ◽  
pp. 156-160 ◽  
Author(s):  
Feng Li ◽  
Mingqin Chen ◽  
Xinzhi Sun ◽  
Xiuzhong Wang ◽  
Peng Li
Keyword(s):  
Graphene Oxide ◽  
Fluorescence Assay ◽  
Endonuclease Activity ◽  
Rna Endonuclease
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