Potassium-doped graphene enhanced electrochemiluminescence of SiO2@CdS nanocomposites for sensitive detection of TATA-binding protein

2012 ◽  
Vol 48 (51) ◽  
pp. 6429 ◽  
Author(s):  
Jing Wang ◽  
Wei-Wei Zhao ◽  
Xiao-Rong Li ◽  
Jing-Juan Xu ◽  
Hong-Yuan Chen
Keyword(s):  
Binding Protein ◽  
Tata Binding Protein ◽  
Sensitive Detection ◽  
Doped Graphene ◽  
Cds Nanocomposites

scholarly journals Biochemical Characterization of the TATA-binding Protein-Gal4 Activation Domain Complex

2000 ◽  
Vol 275 (41) ◽  
pp. 31914-31920 ◽  
Author(s):  
Yueqing Xie ◽  
Carilee Denison ◽  
Sang-Hwa Yang ◽  
David A. Fancy ◽  
Thomas Kodadek
Keyword(s):  
Biochemical Characterization ◽  
Binding Protein ◽  
Tata Binding Protein ◽  
Activation Domain ◽  
Gal4 Activation Domain

An abnormally acidic TATA-binding protein from a hyperthermophilic archaeon

Gene ◽  
1995 ◽  
Vol 166 (1) ◽  
pp. 139-143 ◽  
Author(s):  
Naeem Rashid ◽  
Masaaki Morikawa ◽  
Tadayuki Imanaka
Keyword(s):  
Binding Protein ◽  
Tata Binding Protein ◽  
Hyperthermophilic Archaeon

scholarly journals DNA Sequence-dependent Differences in TATA-binding Protein-induced DNA Bending in Solution Are Highly Sensitive to Osmolytes

2001 ◽  
Vol 276 (18) ◽  
pp. 14623-14627 ◽  
Author(s):  
Jiong Wu ◽  
Kay M. Parkhurst ◽  
Robyn M. Powell ◽  
Lawrence J. Parkhurst
Keyword(s):  
Dna Sequence ◽  
Binding Protein ◽  
Dna Bending ◽  
Tata Binding Protein ◽  
Sequence Dependent ◽  
Highly Sensitive

Mechanism of initiator-mediated transcription: evidence for a functional interaction between the TATA-binding protein and DNA in the absence of a specific recognition sequence

1993 ◽  
Vol 13 (7) ◽  
pp. 3841-3849
Author(s):  
B Zenzie-Gregory ◽  
A Khachi ◽  
I P Garraway ◽  
S T Smale
Keyword(s):  
Transcription Initiation ◽  
Binding Protein ◽  
Tata Binding Protein ◽  
Upstream Region ◽  
Promoter Strength ◽  
Recognition Sequence ◽  
Specific Recognition ◽  
Rate Limiting

Promoters containing Sp1 binding sites and an initiator element but lacking a TATA box direct high levels of accurate transcription initiation by using a mechanism that requires the TATA-binding protein (TBP). We have begun to address the role of TBP during transcription from Sp1-initiator promoters by varying the nucleotide sequence between -14 and -33 relative to the start site. With each of several promoters containing different upstream sequences, we detected accurate transcription both in vitro and in vivo, but the promoter strengths varied widely, particularly with the in vitro assay. The variable promoter activities correlated with, but were not proportional to, the abilities of the upstream sequences to function as TATA boxes, as assessed by multiple criteria. These results confirm that accurate transcription can proceed in the presence of an initiator, regardless of the sequence present in the -30 region. However, the results reveal a role for this upstream region, most consistent with a model in which initiator-mediated transcription requires binding of TBP to the upstream DNA in the absence of a specific recognition sequence. Moreover, in vivo it appears that the promoter strength is modulated less severely by altering the -30 sequence, consistent with a previous suggestion that TBP is not rate limiting in vivo for TATA-less promoters. Taken together, these results suggest that variations in the structure of a core promoter might alter the rate-limiting step for transcription initiation and thereby alter the potential modes of transcriptional regulation, without severely changing the pathway used to assemble a functional preinitiation complex.

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