Background current reduction and biobarcode amplification for label-free, highly sensitive electrochemical detection of pathogenic DNA

2012 ◽  
Vol 48 (27) ◽  
pp. 3309 ◽  
Author(s):  
Jin Xu ◽  
Bingying Jiang ◽  
Jiao Su ◽  
Yun Xiang ◽  
Ruo Yuan ◽  
...  
Keyword(s):  
Electrochemical Detection ◽  
Label Free ◽  
Background Current ◽  
Highly Sensitive ◽  
Current Reduction

Highly sensitive electrochemical detection of circulating tumor DNA based on thin-layer MoS2/graphene composites

RSC Advances ◽  
2016 ◽  
Vol 6 (27) ◽  
pp. 22673-22678 ◽  
Author(s):  
Yilan Chu ◽  
Bin Cai ◽  
Ye Ma ◽  
Minggang Zhao ◽  
Zhizhen Ye ◽  
...  
Keyword(s):  
Thin Layer ◽  
Electrochemical Sensor ◽  
Molybdenum Disulfide ◽  
Electrochemical Detection ◽  
Ultrasonic Method ◽  
Hydrothermal Process ◽  
Circulating Tumor Dna ◽  
Label Free ◽  
Highly Sensitive ◽  
Tumor Dna

By integrating thin-layer molybdenum disulfide (MoS2) and graphene through a hydrothermal process and an ultrasonic method, a label-free, amplification-free and ultrasensitive circulating tumor DNA electrochemical sensor was made.

scholarly journals Optical Monitoring of the Production Quality of Si-Nanoribbon Chips Intended for the Detection of ASD-Associated Oligonucleotides

Micromachines ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 147
Author(s):  
Kristina A. Malsagova ◽  
Tatyana O. Pleshakova ◽  
Vladimir P. Popov ◽  
Igor N. Kupriyanov ◽  
Rafael A. Galiullin ◽  
...  
Keyword(s):  
Production Quality ◽  
Autism Spectrum ◽  
Covalent Immobilization ◽  
Biological Macromolecules ◽  
Label Free ◽  
Raman Scattering Spectroscopy ◽  
Dna Oligonucleotides ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

Gas-phase etching and optical lithography were employed for the fabrication of a silicon nanoribbon chip (Si-NR chip). The quality of the so-fabricated silicon nanoribbons (Si-NRs) was monitored by optical Raman scattering spectroscopy. It was demonstrated that the structures of the Si-NRs were virtually defect-free, meaning they could be used for highly sensitive detection of biological macromolecules. The Si-NR chips were then used for the highly sensitive nanoelectronics detection of DNA oligonucleotides (oDNAs), which represent synthetic analogs of 106a-5p microRNA (miR-106a-5p), associated with the development of autism spectrum disorders in children. The specificity of the analysis was attained by the sensitization of the Si-NR chip sur-face by covalent immobilization of oDNA probes, whose nucleotide sequence was complementary to the known sequence of miR-106a-5p. The use of the Si-NR chip was demonstrated to al-low for the rapid label-free real-time detection of oDNA at ultra-low (~10−17 M) concentrations.

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