Thiol–ene reaction: a versatile tool in site-specific labelling of proteins with chemically inert tags for paramagnetic NMR

2012 ◽  
Vol 48 (21) ◽  
pp. 2704 ◽  
Author(s):  
Qing-Feng Li ◽  
Yin Yang ◽  
Ansis Maleckis ◽  
Gottfried Otting ◽  
Xun-Cheng Su
Keyword(s):  
Paramagnetic Nmr ◽  
Site Specific ◽  
Ene Reaction ◽  
Versatile Tool ◽  
Specific Labelling

Site-Specific Labelling of Native Mammalian Proteins for Single-Molecule FRET Measurements

ChemBioChem ◽  
2018 ◽  
Vol 19 (8) ◽  
pp. 780-783 ◽  
Author(s):  
Alexander Gust ◽  
Leonhard Jakob ◽  
Daniela M. Zeitler ◽  
Astrid Bruckmann ◽  
Kevin Kramm ◽  
...  
Keyword(s):  
Single Molecule ◽  
Site Specific ◽  
Single Molecule Fret ◽  
Mammalian Proteins ◽  
Specific Labelling

Synthesis of novel maleimide derivatives for “site-specific labelling”

2010 ◽  
Vol 37 (6) ◽  
pp. 689
Author(s):  
Verena Nagel ◽  
Carsten Burchardt ◽  
Patrick J. Riss ◽  
Frank Rösch
Keyword(s):  
Site Specific ◽  
Specific Labelling

Site-Specific Labelling of Proteins with a Rigid Lanthanide-Binding Tag

ChemBioChem ◽  
2006 ◽  
Vol 7 (10) ◽  
pp. 1599-1604 ◽  
Author(s):  
Xun-Cheng Su ◽  
Thomas Huber ◽  
Nicholas E. Dixon ◽  
Gottfried Otting
Keyword(s):  
Site Specific ◽  
Specific Labelling

Expanding the genetic code for site-specific labelling of tobacco mosaic virus coat protein and building biotin-functionalized virus-like particles

2014 ◽  
Vol 50 (30) ◽  
pp. 4007-4009 ◽  
Author(s):  
F. C. Wu ◽  
H. Zhang ◽  
Q. Zhou ◽  
M. Wu ◽  
Z. Ballard ◽  
...  
Keyword(s):  
Coat Protein ◽  
Tobacco Mosaic Virus ◽  
Genetic Code ◽  
Mosaic Virus ◽  
Site Specific ◽  
Virus Like Particles ◽  
Specific Modification ◽  
Virus Coat Protein ◽  
Virus Coat ◽  
Specific Labelling

Building biotin-functionalized virus-like particles by combining a genetic code expanding technology and site specific modification of tobacco mosaic virus coat protein.

scholarly journals RNA-CLAMP Enables Photo-activated Control of CRISPR-Cas9 Gene Editing by Site-specific Intramolecular Cross-linking of the sgRNA

2021 ◽  
Author(s):  
Dongyang Zhang ◽  
Shuaijiang Jin ◽  
Luping Liu ◽  
Ember Tota ◽  
Zijie Li ◽  
...  
Keyword(s):  
Dna Cleavage ◽  
Mammalian Cells ◽  
Gene Editing ◽  
Cross Linking ◽  
Stimuli Responsive ◽  
Spatiotemporal Resolution ◽  
Site Specific ◽  
Guide Rna ◽  
Dna Cleavage Activity ◽  
Versatile Tool

AbstractHere we introduce RNA-CLAMP, a technology which enables site-specific and enzymatic cross-linking (clamping) of two selected stem loops within an RNA of interest. Intramolecular clamping of the RNA can disrupt normal RNA function, whereas subsequent photo-cleavage of the crosslinker restores activity. We applied the RNA-CLAMP technique to the single guide RNA of the CRISPR-Cas9 gene editing system. By clamping two stem loops of the single-guide RNA (sgRNA) with a photo-cleavable cross-linker, gene editing was completely silenced. Visible light irradiation cleaved the crosslinker and restored gene editing with high spatiotemporal resolution. Furthermore, by designing two photo-cleavable linkers which are responsive to different wavelength of lights, we achieved multiplexed photo-activation of gene editing in mammalian cells. Notably, although the Cas9-sgRNA RNP is not capable of DNA cleavage activity upon clamping, it maintained the capability to bind to the target DNA. The RNA-CLAMP enabled photo-activated CRISPR-Cas9 gene editing platform offers clean background, free choice of activation wavelength and multiplexing capability. We believe that this technology to precisely and rapidly control gene editing will serve as a versatile tool in the future development of stimuli responsive gene editing technologies. Beyond gene editing, RNA-CLAMP provides a site-specific tool for manipulating the internal structure of functional RNAs.

scholarly journals Water-soluble allyl sulfones for dual site-specific labelling of proteins and cyclic peptides

2016 ◽  
Vol 7 (5) ◽  
pp. 3234-3239 ◽  
Author(s):  
Tao Wang ◽  
Andreas Riegger ◽  
Markus Lamla ◽  
Sebastian Wiese ◽  
Patrick Oeckl ◽  
...  
Keyword(s):  
Cyclic Peptides ◽  
Specific Protein ◽  
Water Soluble ◽  
Site Specific ◽  
Protein Modifications ◽  
Dual Site ◽  
Specific Labelling

Allyl sulfones as efficient disulfide rebridging agents for site-specific protein modifications with up to two additional functionalities in water.

Single-armed phenylsulfonated pyridine derivative of DOTA is rigid and stable paramagnetic tag in protein analysis

2016 ◽  
Vol 52 (77) ◽  
pp. 11535-11538 ◽  
Author(s):  
Feng Yang ◽  
Xiao Wang ◽  
Bin-Bin Pan ◽  
Xun-Cheng Su
Keyword(s):  
Long Range ◽  
Protein Analysis ◽  
Pyridine Derivative ◽  
Pyridine Derivatives ◽  
Site Specific ◽  
Protein Conjugates ◽  
Paramagnetic Tag ◽  
Structural Restraints ◽  
Respective Protein ◽  
Specific Labelling

Single-armed DOTA-like phenylsulfonated pyridine derivatives are rigid and stable paramagnetic tags for site-specific labelling of proteins. The respective protein conjugates yield valuable long-range structural restraints for proteins.

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