Preparation of graphene-encapsulated magnetic microspheres for protein/peptide enrichment and MALDI-TOF MS analysis

2012 ◽  
Vol 48 (13) ◽  
pp. 1874 ◽  
Author(s):  
Qian Liu ◽  
Jianbo Shi ◽  
Mengting Cheng ◽  
Guoliang Li ◽  
Dong Cao ◽  
...  
Keyword(s):  
Magnetic Microspheres ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Ms Analysis ◽  
Peptide Enrichment ◽  
Tof Ms

Enrichment of Peptide with Graphene for MALDI-TOF MS Analysis

2013 ◽  
Vol 781-784 ◽  
pp. 111-114
Author(s):  
Neng Sheng Ye ◽  
Ya Li Xie ◽  
Chang Liu ◽  
Jian Li
Keyword(s):  
Signal To Noise Ratio ◽  
Adsorptive Capacity ◽  
Adsorptive Property ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Ms Analysis ◽  
Peptide Enrichment ◽  
Desorption Step ◽  
Tof Ms

In this paper, graphene was used as the adsorbent for peptide enrichment, and followed by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis. To study the adsorptive property of graphene, peptide angiotensin II was chosen as the model target. The enrichment material was analyzed by MALDI-TOF MS directly without desorption step. As a result, graphene showed good adsorptive capacity for peptide. After enrichment, the peak intensity and the signal-to-noise ratio were obviously increased.

The suitability of different DHB isomers as matrices for the MALDI-TOF MS analysis of phospholipids: which isomer for what purpose?

2006 ◽  
Vol 36 (4-5) ◽  
pp. 517-527 ◽  
Author(s):  
Jürgen Schiller ◽  
Rosmarie Süß ◽  
Beate Fuchs ◽  
Matthias Müller ◽  
Marijana Petković ◽  
...  
Keyword(s):  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Ms Analysis ◽  
Tof Ms

NMR, HPLC-ESI-MS, and MALDI-TOF MS Analysis of Condensed Tannins from Delonix regia (Bojer ex Hook.) Raf. and Their Bioactivities

2012 ◽  
Vol 60 (19) ◽  
pp. 5013-5022 ◽  
Author(s):  
Wei-Ming Chai ◽  
Yan Shi ◽  
Hui-Ling Feng ◽  
Ling Qiu ◽  
Hai-Chao Zhou ◽  
...  
Keyword(s):  
Condensed Tannins ◽  
Maldi Tof Ms ◽  
Esi Ms ◽  
Delonix Regia ◽  
Maldi Tof ◽  
Ms Analysis ◽  
Tof Ms

Identification of Lice Stored in Alcohol Using MALDI-TOF MS

2020 ◽  
Author(s):  
Hanene Benyahia ◽  
Basma Ouarti ◽  
Adama Zan Diarra ◽  
Mehdi Boucheikhchoukh ◽  
Mohamed Nadir Meguini ◽  
...  
Keyword(s):  
Economic Consequences ◽  
Correct Identification ◽  
Protein Profiles ◽  
Innovative Strategy ◽  
Maldi Tof Ms ◽  
Pediculus Humanus ◽  
Maldi Tof ◽  
Ms Analysis ◽  
Storage Methods ◽  
Tof Ms

Abstract Lice pose major public and veterinary health problems with economic consequences. Their identification is essential and requires the development of an innovative strategy. MALDI-TOF MS has recently been proposed as a quick, inexpensive, and accurate tool for the identification of arthropods. Alcohol is one of the most frequently used storage methods and makes it possible to store samples for long periods at room temperature. Several recent studies have reported that alcohol alters protein profiles resulting from MS analysis. After preliminary studies on frozen lice, the purpose of this research was to evaluate the influence of alcohol preservation on the accuracy of lice identification by MALDI-TOF MS. To this end, lice stored in alcohol for variable periods were submitted for MS analysis and sample preparation protocols were optimized. The reproducibility and specificity of the MS spectra obtained on both these arthropod families allowed us to implement the reference MS spectra database (DB) with protein profiles of seven lice species stored in alcohol. Blind tests revealed a correct identification of 93.9% of Pediculus humanus corporis (Linnaeus, 1758) and 98.4% of the other lice species collected in the field. This study demonstrated that MALDI-TOF MS could be successfully used for the identification of lice stored in alcohol for different lengths of time.

Identification of Arcanobacterium hippocoleae by MALDI-TOF MS analysis and by various genotypical properties

2017 ◽  
Vol 115 ◽  
pp. 10-12 ◽  
Author(s):  
J.-P. Wickhorst ◽  
O. Sammra ◽  
A.A. Hassan ◽  
M. Alssashen ◽  
C. Lämmler ◽  
...  
Keyword(s):  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Ms Analysis ◽  
Tof Ms
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