Fast HPLC analysis of adenine in human plasma using a new generation C28 column and different extraction methods

2013 ◽  
Vol 5 (6) ◽  
pp. 1487 ◽  
Author(s):  
Mohammed Al Za'abi ◽  
Badreldin H. Ali ◽  
Afzal Hussain ◽  
Imran Ali
Keyword(s):  
Human Plasma ◽  
Hplc Analysis ◽  
Extraction Methods ◽  
New Generation

scholarly journals Development of enzyme immunoassay for endogenous ouabain-like compound in human plasma

1997 ◽  
Vol 43 (5) ◽  
pp. 715-722 ◽  
Author(s):  
Steven Harwood ◽  
John A Little ◽  
Gerard Gallacher ◽  
David Perrett ◽  
Raymond Edwards ◽  
...  
Keyword(s):  
Detection Limit ◽  
Healthy Volunteers ◽  
Enzyme Immunoassay ◽  
Human Plasma ◽  
Hplc Analysis ◽  
Cross Reactivity ◽  
Standard Curve ◽  
Endogenous Ouabain ◽  
Endogenous Steroid ◽  
Hplc Study

Abstract Widespread evidence supports the existence of an endogenous digitalis-like compound in mammals. We report here the development of a novel enzyme immunoassay for ouabain that, in conjunction with a detailed HPLC study, identifies a ouabain-like compound (OLC) in extracted human plasma. The assay is sensitive—minimum detection limit for OLC 37 pmol/L (11 pmol/L in plasma)—and has a working range (between-assay CV <10%) of 180–10 000 pmol/L (54–3000 pmol/L in plasma). Mean recoveries of ouabain added to plasma ranged from 90% to 100%, and plasma extracts diluted in parallel to the standard curve. Plasma OLC concentrations in 10 healthy volunteers averaged 92 pmol/L (range 55–168), assuming 100% cross-reactivity of OLC in the ouabain assay. HPLC analysis with two distinct chromatographic conditions demonstrated that endogenous human plasma OLC co-eluted with authentic ouabain. The enzyme immunoassay is rapid and easy to perform and will support further investigation of the nature of this controversial endogenous steroid.

In situ Surfactant-based Solid Phase Microextraction of p-cresol in Human Plasma Prior to HPLC Analysis

2020 ◽  
Vol 16 (6) ◽  
pp. 687-694
Author(s):  
Azam Samadi ◽  
Abolghasem Jouyban ◽  
Negar Amirhaghiian ◽  
Hamid Tayebi-Khosroshahi
Keyword(s):  
Sample Preparation ◽  
Human Plasma ◽  
Solid Phase Microextraction ◽  
Hplc Analysis ◽  
Solid Phase ◽  
Uremic Toxin ◽  
Preparation Technique ◽  
Plasma Samples ◽  
Waste Products

Background:Uremia is the outcome of the remaining of nitrogenous waste products that are normally removed by the kidneys. Para-cresol (4-methylphenol) can be regarded as a proteinbound uremic toxin. The p-cresol determination in sera is necessary since it is a marker of cardiovascular risk and overall mortality in hemodialysis patients. Among the reported methods, chromatographic ones especially HPLC techniques due to the high sensitivity, selectivity and reproducibility have been extensively exploited in analysis of p-cresol in complex mixtures. However, an appropriate sample preparation prior to analysis is necessary for obtaining accurate and precise results.Methods:In this study, the appropriate precipitating agent for p-cresol determination in plasma samples was investigated. Then, in situ surfactant-based solid phase microextraction followed by HPLCFL detection was developed and validated for the quantification of p-cresol in plasma samples.Results:According to the results, HCl/heat precipitation method was used for p-cresol microextraction from from plasma samples. In situ surfactant-based solid phase microextraction using cetyltrimethylammonium bromide as extraction medium was proposed for pretreatment of plasma samples prior to analysis. The separation was achieved by isocratic elution with sodium acetate buffer (pH 3.8) and acetonitrile (20:80, v/v). Linearity was found to be acceptable over the concentration ranges of 0.5 to 8 μg mL-1 with the limit of detection and quantification of 0.324 and 0.422 μg mL-1, respectively. The variations for intra-day and inter-day precisions were both less than 8.2% and the extraction recoveries were more than 97%.Conclusion:A validated ISS-SPME followed by HPLC-FL detection reported to determine the total p-cresol concentration of human plasma samples. The traditional liquid-liquid extraction techniques are normally time consuming and require the use of large amounts of toxic organic solvents. In addition, the evaporation of extraction solvent and dissolving the analyte in the mobile phase is commonly used before HPLC analysis. Such a requirement makes the sample preparation process even more tedious and time consuming. ISS-SPME that is the developed ISS-SPE in micro scale, is a simple, rapid and effective sample preparation technique that is appropriate for HPLC-FL analysis.

HPLC Analysis of Creatinine in Human Plasma and Urine

1983 ◽  
Vol 21 (6) ◽  
pp. 278-281 ◽  
Author(s):  
R. Achari ◽  
M. Mayersohn ◽  
K. A. Conrad
Keyword(s):  
Human Plasma ◽  
Hplc Analysis ◽  
Human Plasma And Urine
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