Chemical derivatization with a polycyclic aromatic hydrocarbon for highly sensitive detection of N-linked glycans using MALDI-TOF MS

2012 ◽  
Vol 4 (11) ◽  
pp. 3531 ◽  
Author(s):  
Wei Tong ◽  
Huanhuan Han ◽  
Zifeng Song ◽  
Cheng Ma ◽  
Yiting Pan ◽  
...  
Keyword(s):  
Polycyclic Aromatic Hydrocarbon ◽  
Aromatic Hydrocarbon ◽  
Sensitive Detection ◽  
Chemical Derivatization ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Highly Sensitive ◽  
Polycyclic Aromatic ◽  
Highly Sensitive Detection ◽  
Tof Ms

Sensitive detection of polycyclic aromatic hydrocarbons with gold colloid coupled chloride ion SERS sensor

The Analyst ◽  
2019 ◽  
Vol 144 (22) ◽  
pp. 6698-6705 ◽  
Author(s):  
Xuegang Gong ◽  
Xiaoyong Liao ◽  
You Li ◽  
Hongying Cao ◽  
Yishu Zhao ◽  
...  
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Chloride Ion ◽  
Sensitive Detection ◽  
Gold Colloid ◽  
Highly Sensitive ◽  
Polycyclic Aromatic ◽  
Highly Sensitive Detection

Highly sensitive detection of PAH by non-functionally modified gold colloid was realized by chloride ion coupling.

Pretreatment Epidermal Growth Factor Receptor (EGFR) T790M Mutation Predicts Shorter EGFR Tyrosine Kinase Inhibitor Response Duration in Patients With Non–Small-Cell Lung Cancer

2012 ◽  
Vol 30 (4) ◽  
pp. 433-440 ◽  
Author(s):  
Kang-Yi Su ◽  
Hsuan-Yu Chen ◽  
Ker-Chau Li ◽  
Min-Liang Kuo ◽  
James Chih-Hsin Yang ◽  
...  
Keyword(s):  
Direct Sequencing ◽  
Growth Factor Receptor ◽  
T790m Mutation ◽  
Maldi Tof Ms ◽  
Activating Mutations ◽  
Maldi Tof ◽  
Highly Sensitive ◽  
Epidermal Growth ◽  
Tki Treatment ◽  
Tof Ms

Purpose Patients with non–small-cell lung cancer (NSCLC) with epidermal growth factor receptor (EGFR)–activating mutations have excellent response to EGFR tyrosine kinase inhibitors (TKIs), but T790M mutation accounts for most TKI drug resistance. This study used highly sensitive methods to detect T790M before and after TKI therapy and investigated the association of T790M and its mutation frequencies with clinical outcome. Patients and Methods Direct sequencing, matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) and next-generation sequencing (NGS) were used to assess T790M in the following two cohorts of patients with NSCLC: TKI-naive patients (n = 107) and TKI-treated patients (n = 85). Results were correlated with TKI treatment response and survival. Results MALDI-TOF MS was highly sensitive in detecting and quantifying the frequency of EGFR-activating mutations and T790M (detection limits, 0.4% to 2.2%). MALDI-TOF MS identified more T790M than direct sequencing in TKI-naive patients with NSCLC (27 of 107 patients, 25.2% v three of 107 patients, 2.8%, respectively; P < .001) and in TKI-treated patients (before TKI: 23 of 73 patients, 31.5% v two of 73 patients, 2.7%, respectively; P < .001; and after TKI: 10 of 12 patients, 83.3% v four of 12 patients, 33.3%, respectively; P = .0143). The EGFR mutations and their frequencies were confirmed by NGS. T790M was an independent predictor of decreased progression-free survival (PFS) in patients with NSCLC who received TKI treatment (P < .05, multivariate Cox regression). Conclusion T790M may not be a rare event before or after TKI therapy in patients with NSCLC with EGFR-activating mutations. The pretreatment T790M mutation was associated with shorter PFS with EGFR TKI therapy in patients with NSCLC.

Coupling Strong Anion-Exchange Monolithic Capillary with MALDI-TOF MS for Sensitive Detection of Phosphopeptides in Protein Digest

2010 ◽  
Vol 82 (7) ◽  
pp. 2907-2915 ◽  
Author(s):  
Mingming Dong ◽  
Minghuo Wu ◽  
Fangjun Wang ◽  
Hongqiang Qin ◽  
Guanghui Han ◽  
...  
Keyword(s):  
Anion Exchange ◽  
Sensitive Detection ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Strong Anion Exchange ◽  
Tof Ms

A Preliminary Investigation Into the Characterization of Asphaltenes Extracted From an Oil Sand and Two Vacuum Residues From Petroleum Refining Using Nuclear Magnetic Resonance, DEPT, and MALDI-TOF

2017 ◽  
Vol 139 (3) ◽  
Author(s):  
Ce Zheng ◽  
Mingming Zhu ◽  
Wenxu Zhou ◽  
Dongke Zhang
Keyword(s):  
Molecular Weight ◽  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Polycyclic Aromatic Hydrocarbon ◽  
Aromatic Hydrocarbon ◽  
Average Molecular Weight ◽  
Molecular Structures ◽  
Oil Sand ◽  
Maldi Tof ◽  
Polycyclic Aromatic

This paper reports the findings of an investigation into the molecular structures and properties of three asphaltene samples, namely, an asphaltene sample extracted from Buton Oil Sand (Indonesia), and two asphaltene samples extracted from vacuum residues from Liaohe Refinery (China) and Vene Refinery (Venezuela), respectively. The average molecular structural parameters, including the average polycyclic aromatic hydrocarbon (PAH) size, average side chain length, and average molecular weight (AMW), of the three asphaltene samples were estimated using data from nuclear magnetic resonance (NMR) in combination with distortionless enhancement by polarization transfer (DEPT), and then compared against each other. The molecular weight distributions (MWDs) of the three asphaltene samples were measured using a matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. The results indicated that the island molecular architecture predominated in all three asphaltenes and the average polycyclic aromatic hydrocarbon size was found to be six rings. The average molecular weight of the Buton asphaltene sample was found to be ca. 800 Da while those of the two petroleum asphaltene samples were approximately 600 Da. In comparison, the Buton asphaltene sample contained a much higher level of oxygen and sulfur, but a lower aromaticity than those of the two petroleum asphaltene samples. The use of liquid NMR in combination with DEPT was shown to provide an effective method for characterization and estimation of the molecular structures of asphaltenes, supported by MALDI-TOF mass spectra.

scholarly journals Hybrid CuCoO–GO enables ultrasensitive detection of antibiotics with enhanced laser desorption/ionization at nano-interfaces

2019 ◽  
Vol 10 (1) ◽  
pp. 257-267 ◽  
Author(s):  
Enhui Wu ◽  
Kun Feng ◽  
Rui Shi ◽  
Rui Lv ◽  
Fuzhong Ouyang ◽  
...  
Keyword(s):  
Laser Desorption ◽  
Sensitive Detection ◽  
Ultrasensitive Detection ◽  
Laser Desorption Ionization ◽  
Maldi Tof Ms ◽  
Desorption Ionization ◽  
Maldi Tof ◽  
Tof Ms

A hybrid CuCoO–GO matrix allows for sensitive detection of various antibiotics in combination with MALDI TOF MS.

Quantitative analysis of nitro-polycyclic aromatic hydrocarbons in PM2.5 samples with graphene as a matrix by MALDI-TOF MS

2015 ◽  
Vol 7 (9) ◽  
pp. 3967-3971 ◽  
Author(s):  
Nengxuan Ma ◽  
Wei Bian ◽  
Ruijin Li ◽  
Hong Geng ◽  
Jiangang Zhang ◽  
...  
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Quantitative Analysis ◽  
Aromatic Hydrocarbons ◽  
Analysis Method ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Polycyclic Aromatic ◽  
Quantitative Analysis Method ◽  
Nitropolycyclic Aromatic Hydrocarbons ◽  
Tof Ms

In this work, we developed a novel quantitative analysis method for detecting nitropolycyclic aromatic hydrocarbons (nitro-PAHs) in PM2.5 samples with graphene as a matrix using MALDI-TOF MS.

895: Proteomic Analysis of Sera from Bladder Cancer Patients with MALDI-TOF-MS

2007 ◽  
Vol 177 (4S) ◽  
pp. 297-297
Author(s):  
Kristina Schwamborn ◽  
Rene Krieg ◽  
Ruth Knüchel-Clarke ◽  
Joachim Grosse ◽  
Gerhard Jakse
Keyword(s):  
Bladder Cancer ◽  
Cancer Patients ◽  
Proteomic Analysis ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms
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