Colorimetric multiplexed analysis of mercury and silver ions by using a unimolecular DNA probe and unmodified gold nanoparticles

2012 ◽  
Vol 4 (10) ◽  
pp. 3320 ◽  
Author(s):  
Zhihe Qing ◽  
Xiaoxiao He ◽  
Kemin Wang ◽  
Zhen Zou ◽  
Xue Yang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Silver Ions ◽  
Dna Probe ◽  
Multiplexed Analysis

scholarly journals A simple and sensitive sensor for silver ions based on unmodified gold nanoparticles by using dynamic light scattering techniques

2017 ◽  
Vol 95 (12) ◽  
pp. 1267-1272 ◽  
Author(s):  
Zhiyou Xiao ◽  
Anjiang Tang ◽  
Hongsheng Huang ◽  
Ze Wang
Keyword(s):  
Gold Nanoparticles ◽  
Light Scattering ◽  
Dynamic Light Scattering ◽  
High Sensitivity ◽  
Silver Ions ◽  
Hydrodynamic Diameter ◽  
Optimum Conditions ◽  
Sensitivity And Selectivity ◽  
Sensitive Sensor ◽  
Average Hydrodynamic Diameter

A simple and sensitive assay for Ag+ was developed with unmodified gold nanoparticles (AuNPs) by using dynamic light scattering techniques. Ag+ could induce the oligonucleotide (5′-ATC ACT ATA TCA TAT ACT CAT-3′) to change from a single-stranded structure to a double-stranded structure and desorb from the surface of AuNPs, which triggered the aggregation of AuNPs in the salt solution. The average hydrodynamic diameter of aggregated AuNPs could be detected by using dynamic light scattering techniques. Under the optimum conditions, the average hydrodynamic diameter of AuNPs is proportional to the concentration of Ag+ within the range of 13.3–100.0 nmol/L, with a detection limit of 3.2 nmol/L. The method is easy to operate and has low sample consumption, high sensitivity and selectivity.

Development of Silver-Coated Gold Nanoparticles and its Conjugation for Labeling on Lateral Flow Immunoassay

2014 ◽  
Vol 1024 ◽  
pp. 273-276
Author(s):  
Siti Rabizah Makhsin ◽  
Khairunisak Abdul Razak ◽  
Rahmah Noordin
Keyword(s):  
Gold Nanoparticles ◽  
Growth Stage ◽  
Silver Ions ◽  
Biological Properties ◽  
Lateral Flow ◽  
Synthesis Process ◽  
Lateral Flow Immunoassay ◽  
Minimum Concentration ◽  
Colloidal Form ◽  
Washing Process

This work describes the formation of silver-coated gold nanoparticles (Ag-AuNPs) using the seeding-growth method. 15 nm AuNPs seeds were synthesized using the citrate reduction method. In the growth stage, the adding sequence of seeds, ascorbic acid (AA), gold chloride (HAuCl4) and silver nitrate (AgNO3) was explored. The effect AgNO3volume (20 mM) was varied at 0.10, 0.25, 0.50, 1.00, 1.25 and 1.50 ml. Morphology of Ag-AuNPs was observed using a Transmission Electron Microscope (TEM) while UV-Vis Spectrophotometer was used to study the concentration and absorption spectra of colloidal Ag-AuNPs. Zeta-sizer analysis was used to study the particle size distribution of Ag-AuNPs in colloidal form. In the growth stage, the optimum adding sequence was found by adding AA as a reducing agent into AuNPs seeds followed by the addition of HAuCl4and AgNO3. This reshuffle sequence is chosen due to the presence of two absorbance peaks at 398 nm and 501 nm compared to others. From TEM images, increasing concentration of silver ions affected the optical properties, end size of Ag-AuNPs as well as raises the concentration of the colloids suspension. The biological properties of samples Ag-AuNPs (0.1 ml to 0.5 ml AgNO3) were studied by performing the conjugation process between samples and antibody; goat anti-human IgA (GaHIgA). An additional washing process was required to perform conjugation for Ag-AuNPs in order to remove by-product or unreacted chemical produced during synthesis process. The Ag-AuNPs was successfully conjugated with GaHIgA and required a minimum concentration of antibody (9µg/ml) after washing process. In addition, the washed Ag-AuNPs also improved its binding capability with GaHIgA by giving higher intense signal when tested with lined Human IgA on the lateral flow immunoassay (LFI) compared to un-washed Ag-AuNPs.

Shape and SPR Evolution of Thorny Gold Nanoparticles Promoted by Silver Ions

2007 ◽  
Vol 19 (7) ◽  
pp. 1592-1600 ◽  
Author(s):  
Hong Yuan ◽  
Wanhong Ma ◽  
Chuncheng Chen ◽  
Jincai Zhao ◽  
Jiangwen Liu ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Silver Ions

The functionalization of gold nanoparticles as a novel platform for the highly efficient electrochemical detection of silver ions

The Analyst ◽  
2021 ◽  
Author(s):  
Zhourui Xiao ◽  
Han Meng ◽  
Xuefei Qin ◽  
Xueqing Sang ◽  
Yun Zhang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Electrochemical Detection ◽  
Silver Ions ◽  
Highly Efficient

A novel platform was constructed by the functionalization of gold nanoparticles for the highly efficient electrochemical detection of silver ions.

Preparation of Controllable Core−Shell Gold Nanoparticles and Its Application in Detection of Silver Ions

2011 ◽  
Vol 3 (2) ◽  
pp. 183-190 ◽  
Author(s):  
Haowen Huang ◽  
Caiting Qu ◽  
Xuanyong Liu ◽  
Shaowen Huang ◽  
Zhongjian Xu ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Silver Ions ◽  
Core Shell

Electrochemiluminescent molecular logic gates based on MCNTs for the multiplexed analysis of mercury(ii) and silver(i) ions

RSC Advances ◽  
2016 ◽  
Vol 6 (31) ◽  
pp. 26147-26154 ◽  
Author(s):  
Haiyun Liu ◽  
Lina Zhang ◽  
Meng Li ◽  
Mei Yan ◽  
Mei Xue ◽  
...  
Keyword(s):  
Silver Ions ◽  
Logic Gates ◽  
Mercury Ions ◽  
Molecular Logic ◽  
Molecular Logic Gates ◽  
Selective Analysis ◽  
Multiplexed Analysis

In this paper, logic gates with electrochemiluminescence (ECL) signal as outputs were constructed based on the use of the thymine (T)-rich (S1) or cytosine (C)-rich (S2) oligonucleotides for the selective analysis of mercury ions (Hg2+) or silver ions (Ag+).

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