Hierarchical Fe-ZSM-5 zeolite monolithic column for enhanced phosphorylated protein immobilization and identification

2012 ◽  
Vol 4 (9) ◽  
pp. 2644 ◽  
Author(s):  
Wenzhong Zhang ◽  
Deju Wang ◽  
Huai Sun ◽  
Jun Yao ◽  
Fang Xu ◽  
...  
Keyword(s):  
Monolithic Column ◽  
Protein Immobilization ◽  
Phosphorylated Protein

Comparison of CIM® C4-HLD monolithic column with Sartobind phenyl membrane column for pIDKE2 purification

2017 ◽  
Vol 35 (10) ◽  
pp. 1028
Author(s):  
Limonta Miladys ◽  
ZumalacÁRregui Lourdes ◽  
Vidic Urska ◽  
Lendero Krajnc Nika
Keyword(s):  
Monolithic Column

Analysis of diuretics by capillary electrochromatography using poly(1-hexadecene-co-TMPTMA) monolithic column

2010 ◽  
Vol 28 (3) ◽  
pp. 253-259 ◽  
Author(s):  
Minghua LU ◽  
Xin LI ◽  
Qiang FENG ◽  
Guonan CHEN ◽  
Lan ZHANG
Keyword(s):  
Capillary Electrochromatography ◽  
Monolithic Column

Sequential Injection Chromatography with Monolithic Column for Phenothiazines Assay in Human Urine and Pharmaceutical Formulations

2020 ◽  
Vol 16 (7) ◽  
pp. 967-975
Author(s):  
Abubakr M. Idris
Keyword(s):  
Phase Composition ◽  
Human Urine ◽  
Mobile Phase ◽  
Monolithic Column ◽  
Pharmaceutical Formulations ◽  
Pharmaceutical Formulation ◽  
Assay Method ◽  
Sequential Injection ◽  
Mobile Phase Composition ◽  
Sequential Injection Chromatography

Methods: Sequential injection chromatography (SIC) with monolithic column has been proposed with potential benefits for separation and quantification. Objective: To utilize SIC to develop a new assay method for the separation and quantification of some phenothiazines (promethazine, chlorpromazine and perphenazine) in human urine and synthetic pharmaceutical formulations. Methods: The 32 full-factorial design was adopted to study the effect of mobile phase composition on separation efficiency, retention time, peak height and baseline. The separation was conducted on a C18 monolithic column (100 × 4.6 mm) using a mobile phase composition of phosphate: acetonitrile:methanol (60:28:12) at pH 4.0. The detection was carried out using a miniaturized fiber optic spectrometer at 250 nm. Results: Satisfactory analytical features, including number of theoretical plates (1809-6232), peak symmetry (1.0-1.3), recovery (95.5-99.1% in pharmaceutical formulations and 91.6-94.7% in urine), intra-day precision (0.36-1.60% for pharmaceutical formulation and 2.96-3.67 for urine), inter-day precision (1.47-2.28% for pharmaceutical formulation), limits of detection (0.23-0.88 μg/ml) and limits of quantification (0.77-2.90 μg/ml), were obtained. Conclusion: The remarkable advantages of the proposed SIC method are the inexpensiveness in terms of instrumentation and reagent consumption.

scholarly journals pp75: A novel tyrosine-phosphorylated protein that heralds differentiation of HL-60 cells

1991 ◽  
Vol 266 (18) ◽  
pp. 11890-11895
Author(s):  
I. Bushkin ◽  
J. Roth ◽  
D. Heffetz ◽  
Y. Zick
Keyword(s):  
Phosphorylated Protein ◽  
Tyrosine Phosphorylated Protein
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