Monitoring intracellular nitric oxide production using microchip electrophoresis and laser-induced fluorescence detection

2012 ◽  
Vol 4 (2) ◽  
pp. 414 ◽  
Author(s):  
Emilie R. Mainz ◽  
Dulan B. Gunasekara ◽  
Giuseppe Caruso ◽  
Derek T. Jensen ◽  
Matthew K. Hulvey ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Fluorescence Detection ◽  
Microchip Electrophoresis ◽  
Laser Induced Fluorescence ◽  
Nitric Oxide Production ◽  
Laser Induced Fluorescence Detection ◽  
Oxide Production

scholarly journals Single-photon laser-induced fluorescence detection of nitric oxide at sub-parts-per-trillion mixing ratios

2020 ◽  
Vol 13 (5) ◽  
pp. 2425-2439 ◽  
Author(s):  
Andrew W. Rollins ◽  
Pamela S. Rickly ◽  
Ru-Shan Gao ◽  
Thomas B. Ryerson ◽  
Steven S. Brown ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Detection Limit ◽  
Fluorescence Detection ◽  
Narrow Band ◽  
Single Photon ◽  
Spectral Feature ◽  
Laser Induced Fluorescence ◽  
Mixing Ratios ◽  
Laser Induced Fluorescence Detection ◽  
Direct Measurements

Abstract. We describe a newly developed single-photon laser-induced fluorescence sensor for measurements of nitric oxide (NO) in the atmosphere. Rapid tuning of a narrow-band laser on and off of a rotationally resolved NO spectral feature near 215 nm and detection of the red-shifted fluorescence provides for interference-free direct measurements of NO with a detection limit of 1 part per trillion by volume (pptv) for 1 s of integration, or 0.3 pptv for 10 s of integration. Uncertainty in the sensitivity of the instrument is typically ±6–9 %, with no known interferences. Uncertainty in the zero of the detector is shown to be <0.2 pptv. The instrument was deployed on the NASA DC-8 aircraft during the NASA/NOAA FIREX-AQ experiment (Fire Influence on Regional to Global Environments Experiment – Air Quality) during July–September 2019 and provided more than 140 h of NO measurements over 22 flights, demonstrating the ability of this instrument to operate routinely and autonomously. Comparisons with a seasoned chemiluminescence sensor during FIREX-AQ in a variety of chemical environments provides validation and confidence in the accuracy of this technique.

scholarly journals Optimization of a microchip electrophoresis method with electrochemical detection for the determination of nitrite in macrophage cells as an indicator of nitric oxide production

2019 ◽  
Vol 11 (2) ◽  
pp. 148-156 ◽  
Author(s):  
Joseph M. Siegel ◽  
Kelci M. Schilly ◽  
Manjula B. Wijesinghe ◽  
Giuseppe Caruso ◽  
Claudia G. Fresta ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Electrochemical Detection ◽  
Microchip Electrophoresis ◽  
Nitric Oxide Production ◽  
Macrophage Cells ◽  
Oxide Production ◽  
Working Electrode ◽  
Electrophoresis Method ◽  
Pt Black

A transient isotachophoresis separation and Pt black working electrode allow detection of nitrite in stimulated macrophage cells with improved sensitivity.

Ultrasensitive nuclease activity and inhibition assay using microchip electrophoresis with laser induced fluorescence detection

2016 ◽  
Vol 8 (8) ◽  
pp. 1852-1857 ◽  
Author(s):  
Yingfeng Qin ◽  
Jingjin Zhao ◽  
Yong Huang ◽  
Shuting Li ◽  
Shulin Zhao
Keyword(s):  
Fluorescence Detection ◽  
Nuclease Activity ◽  
Microchip Electrophoresis ◽  
Laser Induced Fluorescence ◽  
Ultrasensitive Detection ◽  
Inhibition Assay ◽  
Laser Induced Fluorescence Detection ◽  
Electrophoresis Method

A novel microchip electrophoresis method with laser induced fluorescence detection was developed for ultrasensitive detection of nuclease activity and inhibitors.

scholarly journals Laser-induced-fluorescence detection of nitric oxide in high-pressure flames with A–X(0, 2) excitation

1997 ◽  
Vol 36 (15) ◽  
pp. 3227 ◽  
Author(s):  
Christof Schulz ◽  
Volker Sick ◽  
Johannes Heinze ◽  
Winfried Stricker
Keyword(s):  
Nitric Oxide ◽  
High Pressure ◽  
Fluorescence Detection ◽  
Laser Induced Fluorescence ◽  
Laser Induced Fluorescence Detection
Sign in / Sign up

Export Citation Format

Share Document