Determination of antibodies against human growth hormone using a direct immunoassay format and different electrochemical methods

The Analyst ◽  
2013 ◽  
Vol 138 (5) ◽  
pp. 1427 ◽  
Author(s):  
Natalija German ◽  
Asta Kausaite-Minkstimiene ◽  
Justina Kirlyte ◽  
Asta Makaraviciute ◽  
Arunas Ramanavicius ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Human Growth ◽  
Electrochemical Methods ◽  
Direct Immunoassay

scholarly journals ELISA for Determination of Human Growth Hormone: Recognition of Helix 4 Epitopes

2004 ◽  
Vol 2004 (3) ◽  
pp. 143-149 ◽  
Author(s):  
Juliana F. Moura ◽  
Luiz DeLacerda ◽  
Romolo Sandrini ◽  
Fernanda M. Borba ◽  
Denise N. Castelo ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Synthetic Peptide ◽  
Human Growth ◽  
Cross Reactivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immunoaffinity Column ◽  
Receptor Dimerization ◽  
Human Prolactin

Human growth hormone (hGH) signal transduction initiates with a receptor dimerization in which one molecule binds to the receptor through sites 1 and 2. A sandwich enzyme-linked immunosorbent assay was developed for quantifying hGH molecules that present helix 4 from binding site 1. For this, horse anti-rhGH antibodies were eluted by an immunoaffinity column constituted by sepharose-rhGH. These antibodies were purified through a second column with synthetic peptide correspondent tohGH helix 4, immobilized to sepharose, and used as capture antibodies. Those that did not recognize synthetic peptide were used as a marker antibody. The working range was of 1.95 to 31.25 ng/mL of hGH. The intra-assay coefficient of variation (CV) was between 4.53% and 6.33%, while the interassay CV was between 6.00% and 8.27%. The recovery range was between 96.0% to 103.8%. There was no cross-reactivity with human prolactin. These features show that our assay is an efficient method for the determination of hGH.

Multiplexed determination of human growth hormone and prolactin at a label free electrochemical immunosensor using dual carbon nanotube–screen printed electrodes modified with gold and PEDOT nanoparticles

The Analyst ◽  
2014 ◽  
Vol 139 (18) ◽  
pp. 4556-4563 ◽  
Author(s):  
V. Serafín ◽  
G. Martínez-García ◽  
L. Agüí ◽  
P. Yáñez-Sedeño ◽  
J. M. Pingarrón
Keyword(s):  
Growth Hormone ◽  
Carbon Nanotube ◽  
Simultaneous Determination ◽  
Human Growth Hormone ◽  
Human Growth ◽  
Electrochemical Immunosensor ◽  
Label Free ◽  
Screen Printed Electrodes ◽  
First Time

A label-free dual electrochemical immunosensor for simultaneous determination of human growth and prolactin hormones was prepared for the first time.

scholarly journals UHPLC-HRMS determination of some human growth hormone-releasing peptides in urine

2019 ◽  
Vol 23 (4) ◽  
pp. 509-516
Author(s):  
A. Z. Temerdashev ◽  
◽  
E. V. Dmitrieva ◽  
A. A. Azaryan ◽  
D. A. Burmikin ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Human Growth ◽  
Growth Hormone Releasing Peptides

Stability Indicating Determination of Human Growth Hormone by Novel SE–LC Method

2016 ◽  
Vol 99 (5) ◽  
pp. 1266-1272
Author(s):  
Mamdouh R Rezk ◽  
Mostafa A Shehata ◽  
Marwa F Mohamed ◽  
Faten Abdel Aziz Fathalla
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Mobile Phase ◽  
Human Growth ◽  
Downstream Processing ◽  
Size Exclusion ◽  
Official Method ◽  
Size Exclusion Chromatographic ◽  
The Stability

Abstract A selective, rapid size-exclusion chromatographic method was developed and validated for the separation of the human growth hormone (hGH) somatropin from its high-molecular-weight aggregates. Separation was achieved using a nontoxic mobile phase compared with the official method of the European Pharmacopoeia that uses 2-propanol in a mobile phase. The developed method used a YMC-Pack Diol (YMC Karasuma, Kyoto, Japan; 300 × 8.0 mm, 5 μm) analytical column. The mobile phase was formed with a pH 7phosphate buffer that was pumped at a flow rate of 1 mL/min with UV detection at 214 nm. The overall run time was 20 min and the average retention times were found to be 10.21 min for the monomer peak, 9.52 min for the dimer peak, and 9.14 min for the higher aggregate. This method was validated in terms of selectivity, linearity, and intra- and interday variations according to the International Conference on Harmonization guidelines. The developed method was applied as a rapid tool for evaluating the stability of stressed samples of hGH subjected to different temperature, agitation, and repeated freeze–thaw cycles. The developed method was successfully applied for the assessment of the quality and quantity of hGH during downstream processing, formulation, and storage.

Sign in / Sign up

Export Citation Format

Share Document