Fast and continuous-flow separation of DNA-complexes and topological DNA variants in microfluidic chip format

The Analyst ◽  
2013 ◽  
Vol 138 (1) ◽  
pp. 186-196 ◽  
Author(s):  
Martina Viefhues ◽  
Jan Regtmeier ◽  
Dario Anselmetti
Keyword(s):  
Flow Separation ◽  
Microfluidic Chip ◽  
Continuous Flow ◽  
Dna Complexes ◽  
Dna Variants ◽  
Chip Format

Continuous Dielectrophoretic Separation of Multiple Particles in a Microfluidic Chip

2008 ◽  
Author(s):  
Christian Davidson ◽  
Junjie Zhu ◽  
Xiangchun Xuan
Keyword(s):  
Particle Size ◽  
Electric Field ◽  
Flow Separation ◽  
Microfluidic Chip ◽  
Continuous Flow ◽  
Uniform Electric Field ◽  
Dielectrophoretic Force ◽  
Size Dependent ◽  
Multiple Particles ◽  
Dc Dielectrophoresis

We successfully demonstrate that DC dielectrophoresis can be utilized to separate particles of three dissimilar sizes simultaneously in a microfluidic chip. This continuous-flow separation is attributed to the particle size dependent dielectrophoretic force that is generated by the non-uniform electric field around a single insulating hurdle on the channel sidewall.

scholarly journals Continuous-Flow Separation and Efficient Concentration of Foodborne Bacteria from Large Volume Using Nickel Nanowire Bridge in Microfluidic Chip

Micromachines ◽  
2019 ◽  
Vol 10 (10) ◽  
pp. 644 ◽  
Author(s):  
Huo ◽  
Chen ◽  
Wang ◽  
Cai ◽  
Qi ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Flow Separation ◽  
Large Volume ◽  
Microfluidic Chip ◽  
Continuous Flow ◽  
Pathogenic Bacteria ◽  
Microfluidic Channel ◽  
Separation System ◽  
Foodborne Bacteria ◽  
Nickel Nanowire

Separation and concentration of target bacteria has become essential to sensitive and accurate detection of foodborne bacteria to ensure food safety. In this study, we developed a bacterial separation system for continuous-flow separation and efficient concentration of foodborne bacteria from large volume using a nickel nanowire (NiNW) bridge in the microfluidic chip. The synthesized NiNWs were first modified with the antibodies against the target bacteria and injected into the microfluidic channel to form the NiNW bridge in the presence of the external arc magnetic field. Then, the large volume of bacterial sample was continuous-flow injected to the channel, resulting in specific capture of the target bacteria by the antibodies on the NiNW bridge to form the NiNW–bacteria complexes. Finally, these complexes were flushed out of the channel and concentrated in a lower volume of buffer solution, after the magnetic field was removed. This bacterial separation system was able to separate up to 74% of target bacteria from 10 mL of bacterial sample at low concentrations of ≤102 CFU/mL in 3 h, and has the potential to separate other pathogenic bacteria from large volumes of food samples by changing the antibodies.

An extrusion fluidic driving method for continuous-flow polymerase chain reaction on a microfluidic chip

2009 ◽  
Vol 168 (1-2) ◽  
pp. 71-78 ◽  
Author(s):  
Zhang-Run Xu ◽  
Xin Wang ◽  
Xiao-Feng Fan ◽  
Jian-Hua Wang
Keyword(s):  
Polymerase Chain Reaction ◽  
Microfluidic Chip ◽  
Continuous Flow ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Non-Aqueous Continuous-Flow Electrophoresis (NACFE): Separation Complement for Continuous-Flow Organic Synthesis

2019 ◽  
Author(s):  
Nikita A. Ivanov ◽  
Yimo Liu ◽  
Sven Kochmann ◽  
Sergey N. Krylov
Keyword(s):  
Steady State ◽  
Organic Synthesis ◽  
Flow Separation ◽  
Continuous Flow ◽  
Organic Molecules ◽  
Water Electrolysis ◽  
Organic Salt ◽  
Aqueous Electrolytes ◽  
Differential Distribution ◽  
Steady State Operation

<div>Continuous-flow organic synthesis naturally requires continuous-flow separation of reaction components. The most common continuous-flow separation approach is liquid-liquid extraction based on differential distribution of molecules between organic and aqueous phases. This approach has limited selectivity; it can hardly separate different hydrophobic organic molecules from each other. Continuous-flow electrophoresis can facilitate much more selective separation in a single phase, but it is currently limited to aqueous electrolytes which are incompatible with many hydrophobic organic molecules. Further, water electrolysis in aqueous electrolytes results in generation of large volumes of gas making steady-state operation a major technical challenge. Here, we introduce non-aqueous continuous-flow electrophoresis (NACFE) in which the electrolyte is a solution of an organic salt in an aprotic organic solvent. We demonstrate that NACFE can maintain stable separation of multiple species during 10 hours. The non-aqueous nature of NACFE and its ability to support steady-state operation make it suitable for its incorporation into continuous-flow organic synthesis.</div>

scholarly journals Continuous-Flow Synthesis of N-Succinimidyl 4-[18F]fluorobenzoate Using a Single Microfluidic Chip

PLoS ONE ◽  
2016 ◽  
Vol 11 (7) ◽  
pp. e0159303 ◽  
Author(s):  
Hiroyuki Kimura ◽  
Kenji Tomatsu ◽  
Hidekazu Saiki ◽  
Kenji Arimitsu ◽  
Masahiro Ono ◽  
...  
Keyword(s):  
Microfluidic Chip ◽  
Continuous Flow ◽  
Flow Synthesis

Fabrication and Application of PMMA Continuous-Flow PCR Microfluidic Chip with CO2Laser Direct-Writing Ablation Micromachining Technique

2009 ◽  
Vol 36 (5) ◽  
pp. 1239-1245
Author(s):  
祁恒 祁恒 ◽  
王贤松 王贤松 ◽  
陈涛 陈涛 ◽  
马雪梅 马雪梅 ◽  
姚李英 姚李英 ◽  
...  
Keyword(s):  
Microfluidic Chip ◽  
Continuous Flow ◽  
Direct Writing

Continuous-flow separation of nanoparticles by electrostatic sieving at a micro-nanofluidic interface

2011 ◽  
Vol 34 (10) ◽  
pp. 1180-1183 ◽  
Author(s):  
Jan Regtmeier ◽  
Jörg Käsewieter ◽  
Martina Everwand ◽  
Dario Anselmetti
Keyword(s):  
Flow Separation ◽  
Continuous Flow

scholarly journals Continuous-Flow Enzyme Assay on a Microfluidic Chip for Monitoring Glycerol Secretion from Cultured Adipocytes

2009 ◽  
Vol 81 (6) ◽  
pp. 2350-2356 ◽  
Author(s):  
Anna M. Clark ◽  
Kyle M. Sousa ◽  
Colin Jennings ◽  
Ormond A. MacDougald ◽  
Robert T. Kennedy
Keyword(s):  
Microfluidic Chip ◽  
Continuous Flow ◽  
Enzyme Assay
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