Development of a split SNAP-tag protein complementation assay for visualization of protein–protein interactions in living cells

The Analyst ◽  
2012 ◽  
Vol 137 (20) ◽  
pp. 4760 ◽  
Author(s):  
Masayasu Mie ◽  
Tatsuhiko Naoki ◽  
Kentaro Uchida ◽  
Eiry Kobatake
Keyword(s):  
Protein Interactions ◽  
Living Cells ◽  
Protein Protein Interactions ◽  
Complementation Assay ◽  
Protein Complementation Assay ◽  
Protein Complementation

scholarly journals A Flow Cytometry-Based FRET Assay to Identify and Analyse Protein-Protein Interactions in Living Cells

PLoS ONE ◽  
2010 ◽  
Vol 5 (2) ◽  
pp. e9344 ◽  
Author(s):  
Carina Banning ◽  
Jörg Votteler ◽  
Dirk Hoffmann ◽  
Herwig Koppensteiner ◽  
Martin Warmer ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Protein Interactions ◽  
Living Cells ◽  
Protein Protein Interactions

A guide-tag system controlling client enrichment into Y15 peptide-based granules for an in-cell protein recruitment assay

2021 ◽  
Author(s):  
Takayuki Miki ◽  
Masahiro Hashimoto ◽  
Taichi Nakai ◽  
Hisakazu Mihara
Keyword(s):  
Protein Interactions ◽  
Living Cells ◽  
The Self ◽  
Cell Protein ◽  
Protein Protein Interactions ◽  
Self Assembling ◽  
Client Proteins ◽  
Protein Recruitment ◽  
Peptide Tag

A series of guide-tags that can control the enrichment of client proteins into artificial scaffolds constituted by the self-assembling Y15 peptide tag facilitates the analysis of protein–protein interactions in living cells.

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