A novel chiral ligand exchange capillary electrophoresis system with amino acid ionic liquid as ligand and its application in screening d-amino-acid oxidase inhibitors

The Analyst ◽  
2012 ◽  
Vol 137 (18) ◽  
pp. 4235 ◽  
Author(s):  
Xiaoyu Mu ◽  
Li Qi ◽  
Ying Shen ◽  
Haizhi Zhang ◽  
Juan Qiao ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Capillary Electrophoresis ◽  
Amino Acid ◽  
Ligand Exchange ◽  
Acid Oxidase ◽  
Chiral Ligand ◽  
Amino Acid Oxidase ◽  
Capillary Electrophoresis System ◽  
Electrophoresis System ◽  
Amino Acid Ionic Liquid

Enhanced Amine and Amino Acid Analysis Using Pacific Blue and the Mars Organic Analyzer Microchip Capillary Electrophoresis System

2009 ◽  
Vol 81 (7) ◽  
pp. 2537-2544 ◽  
Author(s):  
Thomas N. Chiesl ◽  
Wai K. Chu ◽  
Amanda M. Stockton ◽  
Xenia Amashukeli ◽  
Frank Grunthaner ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Amino Acid ◽  
Amino Acid Analysis ◽  
Microchip Capillary Electrophoresis ◽  
Capillary Electrophoresis System ◽  
Electrophoresis System

Purification and Characterization of Lupus Anticoagulant Like Protein from Agkistrodon Halys Brevicaudus Venom

1996 ◽  
Vol 76 (06) ◽  
pp. 0993-0997
Author(s):  
Zhao-Yan Li ◽  
Xiao-Wei Wu ◽  
Tie-Fu Yu ◽  
Eric C-Y Lian
Keyword(s):  
Amino Acid ◽  
Lupus Anticoagulant ◽  
Inhibitory Effect ◽  
Clotting Factor ◽  
Acid Oxidase ◽  
Crude Venom ◽  
Amino Acid Oxidase ◽  
Sds Page ◽  
The Individual ◽  
Reducing Condition

SummaryBy means of CM-Sephadex C-25, DEAE-Sephadex A-50, Sephadex G-200, and Sephadex G-75 chromatographies, a lupus anticoagulant like protein (LALP) from Agkistrodon halys brevicaudus was purified. On SDS-PAGE, the purified LALP had a molecular weight of 25,500 daltons under non-reducing condition and 15,000 daltons under reducing condition. The isoelectric point was pH 5.6. Its N terminal amino acid sequencing revealed a mixture of 2 sequences: DCP(P/S)(D/G)WSSYEGH(C/R)Q(Q/K). It was devoid of phospho-lipaseA, fibrino(geno)lytic, 5′-nucleotidase, L-amino acid oxidase, phosphomonoesterase, phosphodiesterase and thrombin-like activities, which were found in crude venom. In the presence of LALP, PT, aPTT, and dRVVT of human plasma were markedly prolonged and its effects were concentration-dependent but time-independent. The inhibitory effect of LALP on the plasma clotting time was enhanced by decreasing phospholipid concentration in TTI test. The individual clotting factor activity was not affected by LALP when higher dilutions of LALP-plasma mixture were used for assay. Russell’s viper venom time was shortened when high phospholipid confirmatory reagent was used. Therefore, the protein has lupus anticoagulant property.

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