Ultrasensitive colorimetric detection of heparin based on self-assembly of gold nanoparticles on graphene oxide

The Analyst ◽  
2012 ◽  
Vol 137 (16) ◽  
pp. 3653 ◽  
Author(s):  
Xiuli Fu ◽  
Lingxin Chen ◽  
Jinhua Li
Nanomaterials ◽  
2018 ◽  
Vol 8 (12) ◽  
pp. 993 ◽  
Author(s):  
Baoyan Wu ◽  
Shihua Hou ◽  
Yongyong Xue ◽  
Zhan Chen

The study reports a facile and eco-friendly approach for nanomaterial synthesis and enzyme immobilization. A corresponding glucose biosensor was fabricated by immobilizing the gold nanoparticles (AuNPs) and glucose oxidase (GOD) multilayer films onto the polypyrrole (PPy)/reduced graphene oxide (RGO) modified glassy carbon electrode (GCE) via the electrodeposition and self-assembly. PPy and graphene oxide were first coated on the surface of a bare GCE by the electrodeposition. Then, AuNPs and GOD were alternately immobilized onto PPy-RGO/GCE electrode using the electrodeposition of AuNPs and self-assembly of GOD to obtain AuNPs-GOD multilayer films. The resulting PPy-RGO-(AuNPs-GOD)n/GCE biosensors were used to characterize and assess their electrocatalytic activity toward glucose using cyclic voltammetry and amperometry. The response current increased with the increased number of AuNPs-GOD layers, and the biosensor based on four layers of AuNPs-GOD showed the best performance. The PPy-RGO-(AuNPs-GOD)4/GCE electrode can detect glucose in a linear range from 0.2 mM to 8 mM with a good sensitivity of 0.89 μA/mM, and a detection limit of 5.6 μM (S/N = 3). This study presents a promising eco-friendly biosensor platform with advantages of electrodeposition and self-assembly, and would be helpful for the future design of more complex electrochemical detection systems.


2020 ◽  
Vol 601 ◽  
pp. 117905 ◽  
Author(s):  
Jiwoong Heo ◽  
Moonhyun Choi ◽  
Daheui Choi ◽  
Hyejoong Jeong ◽  
Hyun Young Kim ◽  
...  

2014 ◽  
Vol 6 (9) ◽  
pp. 985-990
Author(s):  
Asit Baran Bhattacharya ◽  
Suvajit Bakuli ◽  
Purnesh Chattopadhyay ◽  
Soumitra Das ◽  
Sumanta Bera ◽  
...  

2013 ◽  
Vol 85 (23) ◽  
pp. 11456-11463 ◽  
Author(s):  
Yinhui Li ◽  
Yu Duan ◽  
Jing Zheng ◽  
Jishan Li ◽  
Wenjie Zhao ◽  
...  

2019 ◽  
Vol 43 (22) ◽  
pp. 8502-8507 ◽  
Author(s):  
Natpapas Wiriyachaiporn ◽  
Petpimon Srisurat ◽  
Jarumeth Cherngsuwanwong ◽  
Natcha Sangsing ◽  
Jaruwan Chonirat ◽  
...  

Here, we report a simple colorimetric detection method for the determination of protamine based on the self-assembly of gold nanorods (AuNRs) on graphene oxide (GO).


2016 ◽  
Vol 8 (38) ◽  
pp. 6965-6973 ◽  
Author(s):  
Sanjay Kumar ◽  
Pulak Bhushan ◽  
Shantanu Bhattacharya

A novel paper-based device for colorimetric detection of uric acid was developed based on the peroxidase-like activity of gold nanoparticles–graphene conjugates (AuNPs–GO).


2019 ◽  
Vol 56 (2) ◽  
pp. 309-314
Author(s):  
Alexandra Georgiana Vatui (Moise) ◽  
Sorina Nicoleta Valsan ◽  
Corneliu Fratila ◽  
Andreea Nicoleta Ghita ◽  
Arcadii Sobetkii ◽  
...  

The purpose of this experimental laboratory work is to obtain composite membranes having carbon and gold nanoparticles in the matrix. Consideration was made of using graphene oxide, a material which, under certain conditions, can generate membranes by a self-assembly process under vacuum in an aqueous or organic medium. The cellulose acetate (CA) - graphene oxide (GO) composite membranes have been functionalized with gold nanoparticles (AuNP) by two technological processes: thermal evaporation and ultrasonic mixing of stable graphene oxide and gold suspensions. The stable suspensions containing gold nanoparticles were obtained by chemical reduction of aqueous solutions of Tetrachloroauric acid trihydrate (HAuCl4) precursors with sodium citrate under high temperature conditions. Dispersibility of carbon nanostructures and precious metal nanoparticles has been studied with a DLS granulometer - Malvern Instruments Zetasizer ZS90. It was determined the mean particle size and Zeta potential function of the suspensions pH. The graphite based membranes were further subjected to various characterizations techniques. It was found that both the surfaces and the cross-sections were functionalized with gold particles. The presence of Au particles was revealed both on the membrane surface and also inside its structure. Hydrophilic tests demonstrate that the membrane filters have potential to be used in various applications.


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