An indicator-displacement assay for naked-eye detection and quantification of histidine in human urine

The Analyst ◽  
2012 ◽  
Vol 137 (9) ◽  
pp. 2124 ◽  
Author(s):  
Shao-Kai Sun ◽  
Kai-Xiong Tu ◽  
Xiu-Ping Yan
Keyword(s):  
Human Urine ◽  
Eye Detection ◽  
Naked Eye ◽  
Displacement Assay ◽  
Naked Eye Detection ◽  
Detection And Quantification

Anthracene labeled poly(pyridine methacrylamide) as a polymer-based chemosensor for detection of pyrophosphate (P2O74−) in semi-aqueous media

2020 ◽  
Vol 12 (47) ◽  
pp. 5699-5708
Author(s):  
Saswati Ghosh Roy ◽  
Subhendu Mondal ◽  
Kumaresh Ghosh
Keyword(s):  
Detection Limit ◽  
Aqueous Media ◽  
Eye Detection ◽  
Fluorescence Sensing ◽  
Anion Sensing ◽  
Aqueous Dmso ◽  
Naked Eye ◽  
Displacement Assay ◽  
Naked Eye Detection

Two polymers, linear P1 and cross-linked P2, are employed in anion sensing. P1 exhibits fluorescence sensing of HPPi with a detection limit of about 1.63 ppm in aqueous DMSO. P2 exhibits selective naked-eye detection of PPi/HPPi via an indicator displacement assay (IDA).

scholarly journals Colorimetric, Naked-Eye Detection of Lysozyme in Human Urine with Gold Nanoparticles

Nanomaterials ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 612
Author(s):  
Paula M. Castillo ◽  
Francisco J. Fernández-Acejo ◽  
Jose M. Carnerero ◽  
Rafael Prado-Gotor ◽  
Aila Jimenez-Ruiz
Keyword(s):  
Human Urine ◽  
Color Difference ◽  
Gold Colloid ◽  
Eye Detection ◽  
Naked Eye ◽  
Colorimetric Response ◽  
Salt Addition ◽  
Fixed Reference ◽  
Naked Eye Detection

The stabilizing effect of lysozymes to salt addition over a gold colloid are exploited in order to detect lysozymes in human urine samples. The present research is aimed at the development of a fast, naked-eye detection test for urinary lysozymuria, in which direct comparison with a colorimetric reference, allows for the immediate determination of positive/negative cases. CIEL*a*b* parameters were obtained from sample absorbance measurements, and their color difference with respect to a fixed reference point was measured by calculating the ΔE76 parameter, which is a measure of how well the colors can be distinguished by an untrained observer. Results show that a simple and quick test can reliably, in less than 15 min, give a positive colorimetric response in the naked eye for concentrations of a urinary lysozyme over 57.2 µg/mL. This concentration is well within the limits of that observed for leukemia-associated lysozymurias, among other disorders.

scholarly journals Anion Complexation Studies of 3-Nitrophenyl-Substituted Tripodal Thiourea Receptor: A Naked-Eye Detection of Sulfate via Fluoride Displacement Assay

ACS Omega ◽  
2017 ◽  
Vol 2 (12) ◽  
pp. 9057-9066 ◽  
Author(s):  
Maryam Emami Khansari ◽  
Mohammad H. Hasan ◽  
Corey R. Johnson ◽  
Nya A. Williams ◽  
Bryan M. Wong ◽  
...  
Keyword(s):  
Eye Detection ◽  
Naked Eye ◽  
Anion Complexation ◽  
Complexation Studies ◽  
Displacement Assay ◽  
Naked Eye Detection

scholarly journals A Molecular Logic Gate for the Naked-Eye Detection of Gluthatione or Pyrophosphate with a Nickel Based Bio-Sensor

2020 ◽  
Author(s):  
Thibaud Rossel
Keyword(s):  
Michael Addition ◽  
Click Reaction ◽  
Logic Gate ◽  
Water Soluble ◽  
Eye Detection ◽  
Naked Eye ◽  
Molecular Logic Gate ◽  
Molecular Logic ◽  
Displacement Assay ◽  
Naked Eye Detection

<div><div><div><p>An inorganic water soluble Ni(II) complex offers a molecular logic gate for the naked-eye detection of pyrophosphate versus thiol derivatives. Pyrocathecol coordinated to nickel performs a thiol-michael addition click reaction in the presence of thiol derivativs whereas pyrophosphate allows a displacement of the indicator PCV leading to an indicator displacement assay.</p></div></div></div>

scholarly journals A Molecular Logic Gate for the Naked-Eye Detection of Gluthatione or Pyrophosphate with a Nickel Based Bio-Sensor

2020 ◽  
Author(s):  
Thibaud Rossel
Keyword(s):  
Michael Addition ◽  
Click Reaction ◽  
Logic Gate ◽  
Water Soluble ◽  
Eye Detection ◽  
Naked Eye ◽  
Molecular Logic Gate ◽  
Molecular Logic ◽  
Displacement Assay ◽  
Naked Eye Detection

<div><div><div><p>An inorganic water soluble Ni(II) complex offers a molecular logic gate for the naked-eye detection of pyrophosphate versus thiol derivatives. Pyrocathecol coordinated to nickel performs a thiol-michael addition click reaction in the presence of thiol derivativs whereas pyrophosphate allows a displacement of the indicator PCV leading to an indicator displacement assay.</p></div></div></div>

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