Electrochemiluminescent detection of Mucin 1 protein and MCF-7 cancer cells based on the resonance energy transfer

The Analyst ◽  
2012 ◽  
Vol 137 (9) ◽  
pp. 2101 ◽  
Author(s):  
W. Wei ◽  
D. F. Li ◽  
X. H. Pan ◽  
S. Q. Liu
Keyword(s):  
Energy Transfer ◽  
Cancer Cells ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Mucin 1 ◽  
Mcf 7

Flavoprotein miniSOG Cytotoxisity Can Be Induced By Bioluminescence Resonance Energy Transfer

Acta Naturae ◽  
2016 ◽  
Vol 8 (4) ◽  
pp. 118-123 ◽  
Author(s):  
E. I. Shramova ◽  
G. M. Proshkina ◽  
S. P. Chumakov ◽  
Yu. M. Khodarovich ◽  
S. M. Deyev
Keyword(s):  
Cell Death ◽  
Energy Transfer ◽  
Cancer Cells ◽  
Cell Line ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Eukaryotic Cells ◽  
Bioluminescence Resonance Energy Transfer ◽  
Transfected Cell Line ◽  
Nanoluc Luciferase

In this study, we investigated the possibility of phototoxic flavoprotein miniSOG (photosensitizer) excitation in cancer cells by bioluminescence occurring when luciferase NanoLuc oxidizes its substrate, furimazine. We have shown that the phototoxic flavoprotein miniSOG expressed in eukaryotic cells in fusion with NanoLuc luciferase is activated in the presence of its substrate, furimazine. Upon such condition, miniSOG possesses photoinduced cytotoxicity and causes a 48% cell death level in a stably transfected cell line.

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