Mechanisms of photodynamic inactivation of a Gram-negative recombinant bioluminescent bacterium by cationic porphyrins

2011 ◽  
Vol 10 (10) ◽  
pp. 1659 ◽  
Author(s):  
Anabela Tavares ◽  
Sandra R. S. Dias ◽  
Carla M. B. Carvalho ◽  
Maria A. F. Faustino ◽  
João P. C. Tomé ◽  
...  
Keyword(s):  
Photodynamic Inactivation ◽  
Gram Negative ◽  
Cationic Porphyrins ◽  
Bioluminescent Bacterium

scholarly journals Potentiation of photoinactivation of Gram-positive and Gram-negative bacteria mediated by six phenothiazinium dyes by addition of azide ion

2014 ◽  
Vol 13 (11) ◽  
pp. 1541-1548 ◽  
Author(s):  
Kamola R. Kasimova ◽  
Magesh Sadasivam ◽  
Giacomo Landi ◽  
Tadeusz Sarna ◽  
Michael R. Hamblin
Keyword(s):  
Singlet Oxygen ◽  
Hydroxyl Radicals ◽  
Gram Negative Bacteria ◽  
Photodynamic Inactivation ◽  
Gram Positive ◽  
Gram Negative ◽  
Azide Ion

Antimicrobial photodynamic inactivation (APDI) using six different phenothiazinium dyes is mediated by singlet oxygen (quenched by azide) and hydroxyl radicals (potentiated by azide) depending on Gram-classification of the bacteria and whether the dye is washed from the cells.

scholarly journals Cationic Porphyrins as Effective Agents in Photodynamic Inactivation of Opportunistic Plumbing Pathogen Legionella pneumophila

2020 ◽  
Vol 21 (15) ◽  
pp. 5367 ◽  
Author(s):  
Andrija Lesar ◽  
Martina Mušković ◽  
Gabrijela Begić ◽  
Martin Lončarić ◽  
Dijana Tomić Linšak ◽  
...  
Keyword(s):  
Legionella Pneumophila ◽  
Tap Water ◽  
Health Hazard ◽  
Acanthamoeba Castellanii ◽  
Water Disinfection ◽  
Cell Membrane Permeability ◽  
Photodynamic Inactivation ◽  
Cationic Porphyrins ◽  
Environmental Bacterium ◽  
Legionnaires Disease

Legionella pneumophila is an environmental bacterium, an opportunistic premise plumbing pathogen that causes the Legionnaires’ disease. L. pneumophila presents a serious health hazard in building water systems, due to its high resistance to standard water disinfection methods. Our aim was to study the use of photodynamic inactivation (PDI) against Legionella. We investigated and compared the photobactericidal potential of five cationic dyes. We tested toluidine blue (TBO) and methylene blue (MB), and three 3-N-methylpyridylporphyrins, one tetra-cationic and two tri-cationic, one with a short (CH3) and the other with a long (C17H35) alkyl chain, against L. pneumophila in tap water and after irradiation with violet light. All tested dyes demonstrated a certain dark toxicity against L. pneumophila; porphyrins with lower minimal effective concentration (MEC) values than TBO and MB. Nanomolar MEC values, significantly lower than with TBO and MB, were obtained with all three porphyrins in PDI experiments, with amphiphilic porphyrin demonstrating the highest PDI activity. All tested dyes showed increasing PDI with longer irradiation (0–108 J/cm2), especially the two hydrophilic porphyrins. All three porphyrins caused significant changes in cell membrane permeability after irradiation and L. pneumophila, co-cultivated with Acanthamoeba castellanii after treatment with all three porphyrins and irradiation, did not recover in amoeba. We believe our results indicate the considerable potential of cationic porphyrins as effective anti-Legionella agents.

Photodynamic inactivation of Escherichia coli with cationic ammonium Zn(ii) phthalocyanines

2015 ◽  
Vol 14 (10) ◽  
pp. 1872-1879 ◽  
Author(s):  
Deisy M. G. C. Rocha ◽  
N. Venkatramaiah ◽  
Maria C. Gomes ◽  
Adelaide Almeida ◽  
Maria A. F. Faustino ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Water Soluble ◽  
Gram Negative Bacteria ◽  
Photodynamic Inactivation ◽  
Gram Negative ◽  
Soluble Zinc

The aim of this work was the development of a family of novel water soluble zinc(ii) phthalocyanines (Pc) for the photodynamic inactivation of Gram-negative bacteria.

Corrigendum to “Nucleic acid changes during photodynamic inactivation of bacteria by cationic porphyrins” [Bioorg. Med. Chem. 21 (2013) 4311–4318]

2013 ◽  
Vol 21 (17) ◽  
pp. 5648
Author(s):  
Eliana Alves ◽  
Maria A.F. Faustino ◽  
João P.C. Tomé ◽  
Maria G.P.M.S. Neves ◽  
Augusto C. Tomé ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Photodynamic Inactivation ◽  
Cationic Porphyrins ◽  
Inactivation Of Bacteria

scholarly journals Chitosan Augments Photodynamic Inactivation of Gram-Positive and Gram-Negative Bacteria

2011 ◽  
Vol 55 (5) ◽  
pp. 1883-1890 ◽  
Author(s):  
Tsuimin Tsai ◽  
Hsiung-Fei Chien ◽  
Tze-Hsien Wang ◽  
Ching-Tsan Huang ◽  
Yaw-Bee Ker ◽  
...  
Keyword(s):  
Staphylococcus Epidermidis ◽  
Light Exposure ◽  
Gram Negative Bacteria ◽  
Photodynamic Inactivation ◽  
Gram Positive ◽  
Gram Negative ◽  
Content Type ◽  
Gram Positive Bacteria ◽  
Microbial Infections ◽  
Promising Treatment

ABSTRACTAntimicrobial photodynamic inactivation (PDI) was shown to be a promising treatment modality for microbial infections. This study explores the effect of chitosan, a polycationic biopolymer, in increasing the PDI efficacy against Gram-positive bacteria, includingStaphylococcus aureus,Staphylococcus epidermidis,Streptococcus pyogenes, and methicillin-resistantS. aureus(MRSA), as well as the Gram-negative bacteriaPseudomonas aeruginosaandAcinetobacter baumannii. Chitosan at <0.1% was included in the antibacterial process either by coincubation with hematoporphyrin (Hp) and subjection to light exposure to induce the PDI effect or by addition after PDI and further incubation for 30 min. Under conditions in which Hp-PDI killed the microbe on a 2- to 4-log scale, treatment with chitosan at concentrations of as low as 0.025% for a further 30 min completely eradicated the bacteria (which were originally at ∼108CFU/ml). Similar results were also found with toluidine blue O (TBO)-mediated PDI in planktonic and biofilm cells. However, without PDI treatment, chitosan alone did not exert significant antimicrobial activity with 30 min of incubation, suggesting that the potentiated effect of chitosan worked after the bacterial damage induced by PDI. Further studies indicated that the potentiated PDI effect of chitosan was related to the level of PDI damage and the deacetylation level of the chitosan. These results indicate that the combination of PDI and chitosan is quite promising for eradicating microbial infections.

Nucleic acid changes during photodynamic inactivation of bacteria by cationic porphyrins

2013 ◽  
Vol 21 (14) ◽  
pp. 4311-4318 ◽  
Author(s):  
Eliana Alves ◽  
Maria A.F. Faustino ◽  
João P.C. Tomé ◽  
Maria G.P.M.S. Neves ◽  
Augusto C. Tomé ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Photodynamic Inactivation ◽  
Cationic Porphyrins ◽  
Inactivation Of Bacteria

In vitro photodynamic inactivation of conidia of the phytopathogenic fungus Colletotrichum graminicola with cationic porphyrins

2016 ◽  
Vol 15 (5) ◽  
pp. 673-681 ◽  
Author(s):  
Camila Chevonica Vandresen ◽  
Alan Guilherme Gonçalves ◽  
Diogo Ricardo Bazan Ducatti ◽  
Fabio Seigi Murakami ◽  
Miguel Daniel Noseda ◽  
...  
Keyword(s):  
Phytopathogenic Fungus ◽  
Photodynamic Inactivation ◽  
Colletotrichum Graminicola ◽  
Cationic Porphyrins

Photodynamic inactivation (PDI) of the conidial form of theC. graminicolaemploying five cationic porphyrins.

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